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ID   PPD2614    preliminary; circular DNA; SYN; 2890 BP.
XX
AC   X53127;
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DT   15-AUG-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   C. elegans plasmid vector pPD26.14 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2890
RC   pAST18a from pAST & pUC18
RC   pAST18b from pAST & pUC18
RC   pAST19a from pAST & pUC19
RC   pAST19b from pAST & pUC19
RC   pICT19h from pAST & pIC19H
RC   pICT19r from pAST & pIC19R
RC   pPD26.14 from pAST19b & linker
RA   Fire A.Z., Kondo K., Waterson R.;
RT   "Vectors for low copy transformation of C. elegans";
RL   Nucleic Acids Res. 18:4269-4270(1990).
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RN   [2]
RP   1-2890
RC   pPD26.14
RA   Fire A.Z.;
RT   ;
RL   Submitted (17-MAY-1990) on tape to the EMBL Data Library by:
RL   Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL   115 West University Parkway, Baltimore MD 21210, USA.
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CC   pPD26.14 is made from C. elegans sup-7 sequence (K02459) and
CC   pUC19 sequence (M11662).
CC   NM (pPD26.14)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (C.elegans)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC19)(C.elegans sup-7)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAST19B remove XbaI-NdeI 239bp 185..424,
FT                   \ lacZalpha/2813bp
FT                   SalI 2813bp 430..430
FT                   2. linker SalI-EagI-SpiI-ApaI-AfiII 69bp
FT                   -> pPD26.14 2890bp"
FT   misc_feature    1..69
FT                   /note="synthetic sequence"
FT   misc_feature    70..490
FT                   /note="from pUC19"
FT   misc_feature    491..856
FT                   /note="from C. elegans sup-7 locus"
FT   misc_feature    857..2890
FT                   /note="from pUC19"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 2890 BP; 738 A; 694 C; 694 G; 764 T; 0 other;
     atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagtcgg ccgtacgggc
     ccttaaggcc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     cttcaataat acaattttca gaatacgttt tttgtgggct tgggtatatt gtttttaatg
     ttatacttgc agtcgtgaaa tttgattttc aaatttgtag aaaaatcaag aaaataattg
     caacattcgc ttgtgtcaaa aaccaatttc aacaaatttt cgtgtgagaa atacattacc
     agaaggcatt ttttcacacg attagcattt tggactactt tattaaattt ttgcgtgtaa
     ttttgaatta aattgtatta tattactact taaaaaacaa aaaatttgac cactgagcgg
     atcgaacgcc caacctttcg atctagagtc gaacgcgcta ccattgcgcc aagcagtcat
     gttattctct cttgtcattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     ggaaacagct
//