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ID   PPD2677    preliminary; circular DNA; SYN; 7242 BP.
XX
AC   M34305;
XX
DT   03-JUL-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pPD26.77 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-7242
RC   pPD1.27 from pAST19b & pCH110 & oligo
RC   pPD8.02 from pPD1.27 & oligo
RC   pPD8.33 from pPD8.02 & C.elegans unc-54 gene
RC   pPD15.08 from pPD8.02
RC   pPD16.43 from pPD8.02 & pPD15.08
RC   pPD16.51 from pPD8.33 & pPD15.08
RC   pPD18.32 from pPD16.51 & oligo
RC   pPD21.28 from pPD18.32 & pPD16.43
RC   pPD22.11 from pPD21.28
RC   pPD22.04 from pPD21.28
RC   pPD16.01 from pPD16.43 & pPD18.32
RC   pPD25.47 from pBluescript KS+ & glp-1 gene
RC   pPD26.77 from pPD16.43 & pPD25.47
RC   pPD34.110 from pPD21.28 & oligo
RA   Fire A.Z., Harrison S., Dixon D.;
RT   "A modular set of lacZ fusion vectors for studying gene expression
RT   in Caenorhabditis elegans";
RL   Gene 93:189-198(1990).
XX
RN   [2]
RC   pPD26.77
RA   Fire A.Z.;
RT   ;
RL   Submitted (11-MAY-1990) to GenBank on computer-readable media by:
RL   Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL   115 West University Parkway, Baltimore MD 21210, USA.
XX
CC   NM (pPD26.77)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pBR322)
CC   BR (pPD16.01)(pPD16.43)(pPD16.51)(pPD18.32)(pPD21.28)(pPD22.04)
CC   BR (pPD22.11)(pPD26.14)(pPD1.27)(pPD34.110)(pPD8.02)(pPD8.33)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBluescript KS+ remove EcoRI-SalI 27bp
FT                   \ 708..735, MCS/2937bp
FT                   2. E.coli EcoRI-SalI 10487bp, glp-1 gene
FT                   -> pPD25.47 13424bp
FT                   1. pPD16.43 StuI 5730bp 3245..3245
FT                   2. pPD25.47 BstUI-BstUI 1512bp, 3' glp-1 gene
FT                   -> pPD26.77 7242bp"
FT   misc_recomb     57..58
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     102..103
FT                   /note="synthetic end/E. coli trpS start"
FT   misc_recomb     186..187
FT                   /note="E. coli trpS end/synthetic start"
FT   misc_recomb     190..191
FT                   /note="synthetic end/E. coli lacZ start"
FT   misc_recomb     3184..3185
FT                   /note="E. coli lacZ end/synthetic start"
FT   misc_recomb     3244..3245
FT                   /note="synthetic end/unknown DNA start"
FT   misc_recomb     4756..4757
FT                   /note="unknown DNA end/synthetic start"
FT   misc_recomb     4776..4777
FT                   /note="synthetic end/pUC19 start"
FT   misc_recomb     5025..5026
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     5036..5037
FT                   /note="synthetic end/pUC19 start"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 7242 BP; 1780 A; 1801 C; 1846 G; 1815 T; 0 other;
     atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta
     ccgagctcag aaaaaatgac tgctccaaag aagaagcgta aggtaccggt gggtgaagac
     cagaaacagc acctcgaact gagccgcgat attgcccagc gtttcaacgc gctgtatggc
     gagatcgatc ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
     gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc
     gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc
     tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
     ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
     ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac
     agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc
     gcggtgatgg tgctgcgttg gagtgacggc agttatctgg aagatcagga tatgtggcgg
     atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat
     ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt
     cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa
     acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt
     tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa
     atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa
     gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg
     aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt
     caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt
     aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc
     tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat
     cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg
     cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac
     ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg
     cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg
     cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt
     tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac
     agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc
     ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg
     tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac
     ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag
     cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc
     cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca
     agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa
     ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac
     gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa
     aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa
     atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt
     ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc
     acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac
     gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag
     tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat
     caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg
     gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg
     aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa
     aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac
     atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg
     aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa
     cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg
     aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg
     gcggaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa
     taggcgaaac aaatcatctg acaccaccac cgtctgatgg atcgttctca tctccgtctc
     cacattatta tccgacgact acatcgacac cgaatcgaat ggaaacaagt ccggagtaca
     tgtttaacca tgaaatggtg ggtagatgat tattaaaatg tttaagaaaa ttaaataatt
     tgttttaggc accaccggtc aatgcgatgt ggtatactac accacctcct tatcaagatc
     caaactatcg tcatgtgcct ccaaatactg catttcaaaa tgcagagcaa atgaatggct
     ccttctactg ttaatctatt taattcatta atttttcatt tattgactgt atcccggatg
     tttcttgtcc tcccaacata tctcctaact gctcggttca ttttaaatat gctcatctca
     ctacatcacc cagacactgg tccccacaga gttttttgta tactatttcg ggtcattttt
     cttattctag actaatattg taagctataa gttgtagaat aattattgat ccaaatcaga
     ttaagagtat aagctttgtt ttttctcctt ttctttataa cttgttacaa tttttgaaat
     tccctttttt gacaggcttt tattacactg taactgtgtt tcttatcttg caaacattta
     atgaattgta attctttagt atcttgaggg ctttttgttt ttcgaattat tgaagctcaa
     agttccagtt ttactacgat ccagcgaatt ctcctcattt cgatccgatg caattgactt
     cagatcaata ttggttgcct gaaagaaata attgtgagca tttttgtcaa aaaacagaga
     actcaccatt ctcgaggctc ccgttccagg agcagtactt ggtgatggac acgtagattg
     attaaaccaa accaaaggtt ctttcagagt caacttacag cctcgagcgt agtccgtgat
     agcttctcgc agaacactga aaattggaaa tttattggaa taaaaacttt ttctgcactt
     tatagaataa aaaaatcatg aatttacccg aatttaacct ccgaatcgta gaccaaattg
     tccaagtaga tggaaatcac cttgaacatc ggatgttttt catatgctga aaataaatta
     atgaatttat gtaatttttt aaataattac ttttcaattt ggtgaacaat tcctgcttct
     ttgcataggc atctggacga gtgagtcctt tccaatcaat caatgtggtg tcgacctcga
     gggggggccc ggtacccagc ttttgttccc tttagtgagg gttaattccg agcttggcgt
     aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     taggagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagg taactcacat
     taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     aatgaatcgg ccaacgccta ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg
     tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcggcctt aagggcctcg
     tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     gtggaattgt gagcggataa caatttcaca caggaaacag ct
//