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ID   PPD34110   preliminary; circular DNA; SYN; 6620 BP.
XX
AC   M34306;
XX
DT   03-JUL-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pPD34.110 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6620
RC   pPD1.27 from pAST19b & pCH110 & oligo
RC   pPD8.02 from pPD1.27 & oligo
RC   pPD8.33 from pPD8.02 & C.elegans unc-54 gene
RC   pPD15.08 from pPD8.02
RC   pPD16.43 from pPD8.02 & pPD15.08
RC   pPD16.51 from pPD8.33 & pPD15.08
RC   pPD18.32 from pPD16.51 & oligo
RC   pPD21.28 from pPD18.32 & pPD16.43
RC   pPD22.11 from pPD21.28
RC   pPD22.04 from pPD21.28
RC   pPD16.01 from pPD16.43 & pPD18.32
RC   pPD25.47 from pBluescript KS+ & glp-1 gene
RC   pPD26.77 from pPD16.43 & pPD25.47
RC   pPD34.110 from pPD21.28 & oligo
RA   Fire A.Z., Harrison S., Dixon D.;
RT   "A modular set of lacZ fusion vectors for studying gene expression
RT   in Caenorhabditis elegans";
RL   Gene 93:189-198(1990).
XX
RN   [2]
RC   pPD34.110
RA   Fire A.Z.;
RT   ;
RL   Submitted (11-MAY-1990) to GenBank on computer-readable media by:
RL   Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL   115 West University Parkway, Baltimore MD 21210, USA.
XX
CC   NM (pPD34.110)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pBR322)
CC   BR (pPD16.01)(pPD16.43)(pPD16.51)(pPD18.32)(pPD21.28)(pPD22.04)
CC   BR (pPD22.11)(pPD26.14)(pPD26.77)(pPD1.27)(pPD8.02)(pPD8.33)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pPD21.28 Asp718I-Asp718I 6518bp
FT                   \ 185..6563..140
FT                   2. oligo Asp718I/KpnI-Asp718I/KpnI 180bp
FT                   -> pPD34.110 6620bp"
FT   misc_recomb     57..58
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     237..238
FT                   /note="synthetic end/E. coli trpS start"
FT   misc_recomb     321..322
FT                   /note="E. coli trpS end/synthetic start"
FT   misc_recomb     325..326
FT                   /note="synthetic end/E. coli lacZ start"
FT   misc_recomb     3319..3320
FT                   /note="E. coli lacZ end/synthetic start"
FT   misc_recomb     3379..3380
FT                   /note="synthetic end/unknown DNA start"
FT   misc_recomb     4134..4135
FT                   /note="unknown DNA end/synthetic start"
FT   misc_recomb     4154..4155
FT                   /note="synthetic end/pUC19 start"
FT   misc_recomb     4403..4404
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     4414..4415
FT                   /note="synthetic end/pUC19 start"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 6620 BP; 1592 A; 1665 C; 1731 G; 1632 T; 0 other;
     atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggat
     tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
     gaggaccctt ggagggtacc tcgagaaagc tggcaaaggg ctcttgtcct gctaatcgta
     ctactcttca tcgtcatctt cgttattact gttttgttcg tcataagatc taacaaggta
     ccggtgggtg aagaccagaa acagcacctc gaactgagcc gcgatattgc ccagcgtttc
     aacgcgctgt atggcgagat cgatcccgtc gttttacaac gtcgtgactg ggaaaaccct
     ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct
     gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc
     tacaccaacg taacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg
     acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg
     cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc
     ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc
     ggagaaaacc gcctcgcggt gatggtgctg cgttggagtg acggcagtta tctggaagat
     caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact
     acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta
     ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct
     ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc
     gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa
     ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac
     ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat
     ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat
     catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg
     aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac
     acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc
     atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc
     gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg
     aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat
     ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt
     tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc
     atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc
     cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat
     ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat
     gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc
     cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa
     gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc
     agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat
     ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg
     attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc
     gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag
     tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat
     ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac
     cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact gctgacgccg
     ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc
     cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa
     gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct
     cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat
     ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg
     gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga
     ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat
     ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc
     gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc
     agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa
     gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg
     agcccgtcag tatcggcgga attccaactg agcgccggtc gctaccatta ccaacttgtc
     tggtgtcaaa aataataggg gccgctgtca tcagatcgcc atctcgcgcc cgtgcctctg
     acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc
     ccctattttt gttattatca aaaaaacttc ttcttaattt ctttgttttt tagcttcttt
     taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa
     agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac
     aatgttctgt gtacacttct tatgtttttt ttacttctga taaatttttt ttgaaacatc
     atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa
     tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa
     gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg
     cttttgcttt ttgggggttt cccctattgt ttgtcaagag tttcgaggac ggcgtttttc
     ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt
     ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg
     ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact
     agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt
     taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa
     ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     atttcacaca ggaaacagct
//