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ID   PPD802     preliminary; circular DNA; SYN; 6096 BP.
XX
AC   M34297;
XX
DT   03-JUL-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pPD8.02 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6096
RC   pPD1.27 from pAST19b & pCH110 & oligo
RC   pPD8.02 from pPD1.27 & oligo
RC   pPD8.33 from pPD8.02 & C.elegans unc-54 gene
RC   pPD15.08 from pPD8.02
RC   pPD16.43 from pPD8.02 & pPD15.08
RC   pPD16.51 from pPD8.33 & pPD15.08
RC   pPD18.32 from pPD16.51 & oligo
RC   pPD21.28 from pPD18.32 & pPD16.43
RC   pPD22.11 from pPD21.28
RC   pPD22.04 from pPD21.28
RC   pPD16.01 from pPD16.43 & pPD18.32
RC   pPD25.47 from pBluescript KS+ & glp-1 gene
RC   pPD26.77 from pPD16.43 & pPD25.47
RC   pPD34.110 from pPD21.28 & oligo
RA   Fire A.Z., Harrison S., Dixon D.;
RT   "A modular set of lacZ fusion vectors for studying gene expression
RT   in Caenorhabditis elegans";
RL   Gene 93:189-198(1990).
XX
RN   [2]
RC   pPD8.02
RA   Fire A.Z.;
RT   ;
RL   Submitted (11-MAY-1990) to GenBank on computer-readable media by:
RL   Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL   115 West University Parkway, Baltimore MD 21210, USA.
XX
CC   NM (pPD8.02)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pBR322)
CC   BR (pPD16.01)(pPD16.43)(pPD16.51)(pPD18.32)(pPD21.28)(pPD22.04)
CC   BR (pPD22.11)(pPD26.14)(pPD26.77)(pPD34.110)(pPD1.27)(pPD8.33)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pPD1.27 remove EcoRI-NotI 468bp 3185..3653,
FT                   \ 3' lacZ/6008bp
FT                   2. oligo EcoRI-NotI 88bp
FT                   -> pPD8.02 6096bp"
FT   misc_recomb     57..58
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     102..103
FT                   /note="synthetic end/E. coli trpS start"
FT   misc_recomb     186..187
FT                   /note="E. coli trpS end/synthetic start"
FT   misc_recomb     190..191
FT                   /note="synthetic end/E. coli lacZ start"
FT   misc_recomb     3184..3185
FT                   /note="E. coli lacZ end/synthetic start"
FT   misc_recomb     3275..3276
FT                   /note="synthetic end/unknown DNA start"
FT   misc_recomb     3696..3697
FT                   /note="unknown DNA end/C. elegans sup-7 end"
FT   misc_recomb     4062..4063
FT                   /note="C. elegans sup-7 end/pUC19 start"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 6096 BP; 1458 A; 1571 C; 1616 G; 1451 T; 0 other;
     atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta
     ccgagctcag aaaaaatgac tgctccaaag aagaagcgta aggtaccggt gggtgaagac
     cagaaacagc acctcgaact gagccgcgat attgcccagc gtttcaacgc gctgtatggc
     gagatcgatc ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
     gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc
     gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc
     tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
     ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
     ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac
     agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc
     gcggtgatgg tgctgcgttg gagtgacggc agttatctgg aagatcagga tatgtggcgg
     atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat
     ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt
     cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa
     acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt
     tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa
     atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa
     gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg
     aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt
     caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt
     aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc
     tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat
     cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg
     cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac
     ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg
     cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg
     cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt
     tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac
     agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc
     ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg
     tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac
     ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag
     cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc
     cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca
     agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa
     ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac
     gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa
     aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa
     atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt
     ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc
     acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac
     gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag
     tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat
     caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg
     gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg
     aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa
     aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac
     atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg
     aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa
     cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg
     aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg
     gcggaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa
     taggcctact agtcggccgt acgggccctt aaggccgcct gatgcggtat tttctcctta
     cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     tcatgagaca ataaccctga taaatgcttc aataatacaa ttttcagaat acgttttttg
     tgggcttggg tatattgttt ttaatgttat acttgcagtc gtgaaatttg attttcaaat
     ttgtagaaaa atcaagaaaa taattgcaac attcgcttgt gtcaaaaacc aatttcaaca
     aattttcgtg tgagaaatac attaccagaa ggcatttttt cacacgatta gcattttgga
     ctactttatt aaatttttgc gtgtaatttt gaattaaatt gtattatatt actacttaaa
     aaacaaaaaa tttgaccact gagcggatcg aacgcccaac ctttcgatct agagtcgaac
     gcgctaccat tgcgccaagc agtcatgtta ttctctcttg tcattgaaaa aggaagagta
     tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagct
//