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ID PPD833 preliminary; circular DNA; SYN; 7376 BP.
XX
AC M34298;
XX
DT 03-JUL-1990 (Rel. 5, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pPD8.33 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-7376
RC pPD1.27 from pAST19b & pCH110 & oligo
RC pPD8.02 from pPD1.27 & oligo
RC pPD8.33 from pPD8.02 & C.elegans unc-54 gene
RC pPD15.08 from pPD8.02
RC pPD16.43 from pPD8.02 & pPD15.08
RC pPD16.51 from pPD8.33 & pPD15.08
RC pPD18.32 from pPD16.51 & oligo
RC pPD21.28 from pPD18.32 & pPD16.43
RC pPD22.11 from pPD21.28
RC pPD22.04 from pPD21.28
RC pPD16.01 from pPD16.43 & pPD18.32
RC pPD25.47 from pBluescript KS+ & glp-1 gene
RC pPD26.77 from pPD16.43 & pPD25.47
RC pPD34.110 from pPD21.28 & oligo
RA Fire A.Z., Harrison S., Dixon D.;
RT "A modular set of lacZ fusion vectors for studying gene expression
RT in Caenorhabditis elegans";
RL Gene 93:189-198(1990).
XX
RN [2]
RC pPD8.33
RA Fire A.Z.;
RT ;
RL Submitted (11-MAY-1990) to GenBank on computer-readable media by:
RL Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL 115 West University Parkway, Baltimore MD 21210, USA.
XX
CC NM (pPD8.33)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE (color blue/white)
CC PA (pBR322)
CC BR (pPD16.01)(pPD16.43)(pPD16.51)(pPD18.32)(pPD21.28)(pPD22.04)
CC BR (pPD22.11)(pPD26.14)(pPD26.77)(pPD34.110)(pPD8.02)(pPD1.27)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pPD8.02 StuI 6096bp 3245..3245
FT 2. C.elegans EagI-XbaI 1300bp, unc-54 gene
FT Klenow:Klenow
FT -> pPD8.33 7376bp"
FT misc_recomb 57..58
FT /note="pUC19 end/synthetic start"
FT misc_recomb 102..103
FT /note="synthetic end/E. coli trpS start"
FT misc_recomb 186..187
FT /note="E. coli trpS end/synthetic start"
FT misc_recomb 190..191
FT /note="synthetic end/E. coli lacZ start"
FT misc_recomb 3184..3185
FT /note="E. coli lacZ end/synthetic start"
FT misc_recomb 3244..3245
FT /note="synthetic end/unknown DNA start"
FT misc_recomb 4555..4556
FT /note="synthetic end/pUC19 start"
FT misc_recomb 4976..4977
FT /note="pUC19 end/C. elegans sup-7 start"
FT misc_recomb 5342..5343
FT /note="C. elegans sup-7 end/pUC19 start"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ Sequence 7376 BP; 1863 A; 1793 C; 1814 G; 1906 T; 0 other;
atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta
ccgagctcag aaaaaatgac tgctccaaag aagaagcgta aggtaccggt gggtgaagac
cagaaacagc acctcgaact gagccgcgat attgcccagc gtttcaacgc gctgtatggc
gagatcgatc ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc
gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc
tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac
agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc
gcggtgatgg tgctgcgttg gagtgacggc agttatctgg aagatcagga tatgtggcgg
atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat
ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt
cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa
acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt
tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa
atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa
gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg
aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt
caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt
aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc
tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat
cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg
cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac
ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg
cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg
cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt
tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac
agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc
ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg
tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac
ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag
cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc
cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca
agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa
ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac
gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa
aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa
atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt
ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc
acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac
gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag
tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat
caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg
gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg
aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa
aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac
atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg
aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa
cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg
aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg
gcggaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa
taggggccgc tgtcatcaga tcgccatctc gcgcccgtgc ctctgacttc taagtccaat
tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
tatcaaaaaa acttcttctt aatttctttg ttttttagct tcttttaagt cacctctaac
aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg
tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca
cttcttatgt tttttttact tctgataaat tttttttgaa acatcataga aaaaaccgca
cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc
acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt
tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg
ggtttcccct attgtttgtc aagagtttcg aggacggcgt ttttcttgct aaaatcacaa
gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
tgacagaata cattcccaat ataccaaaca taactgttta aaattaaaca tttttctaaa
ttttatatga tttcttttaa atttgcaaaa attacttaaa tttgaattcc cgcgcaaatg
agtgacttca ttttctgcat tattgtgttt tccggctata ttaataggta tttgtttgtg
tttttcttta ttttatgatt cgaactccaa tttgtaaatt ttcgaacata tttccctaaa
gaaaaaatat gattaatctg gaaaaattgg aaaattattt ttcaaataaa aaacaaagaa
aaaaatgaag aaaaacctat tagtttggcc ataaaacgca aaaatgtcga aaatgacgtc
actcatctgc gcgggaaatc aagaataatt cggccttttt tatttttttg gaaaatcgta
aaacatttag aaaaattttt taatagttat agtgggactg tattctgtca tttagggcaa
aagccagaga cgctactcca ccgttaacat gaattatgaa tattattgcg acaagaccca
aacattgata aaccgcaaat ctagcctact agtcggccgt acgggccctt aaggccgcct
gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatacaa
ttttcagaat acgttttttg tgggcttggg tatattgttt ttaatgttat acttgcagtc
gtgaaatttg attttcaaat ttgtagaaaa atcaagaaaa taattgcaac attcgcttgt
gtcaaaaacc aatttcaaca aattttcgtg tgagaaatac attaccagaa ggcatttttt
cacacgatta gcattttgga ctactttatt aaatttttgc gtgtaatttt gaattaaatt
gtattatatt actacttaaa aaacaaaaaa tttgaccact gagcggatcg aacgcccaac
ctttcgatct agagtcgaac gcgctaccat tgcgccaagc agtcatgtta ttctctcttg
tcattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagct
//