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ID   PPLLAM     preliminary; circular DNA; SYN; 5216 BP.
XX
AC   IG1098; J02459; M17233;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pPL-lambda - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pPL-lambda from lambda, pL promoter]
RC   pPLa2 from pBR322 & lambda, pL promoter
RC   pPLa20 from pPLa2
RC   pPLa23 from pPLa2 & pBR322
RC   pPLa2311 from pPLa23 & pMK20, kan
RC   pPLa8 from pPLa2311 & BamHI linker
RC   pPLa83 from pPLa8 & linker
RC   pPLa831 from pPLa83
RC   pPLa832 from pPLa831
RC   pPLc2 from pPLa832 & pBR322
RC   pPLc23 from pPLc2
RC   pPLc236 from pPLc23 & linker
RC   pPLc28 from pPLc236
RC   pPLc24 from pPLc28 & pMS2-7
RC   [pLc245 from pPLc series]
RA   Remaut E., Stanssens P., Fiers W.;
RT   "Plasmid vectors for high-efficiency expression controlled by the pl
RT   promoter of coliphage lambda";
RL   Gene 15:81-93(1981).
XX
RN   [2]
RC   plambda8 from pBR322 & lambda, gamma-delta transposon
RA   Reed R.R.;
RT   "Transposon-mediated site-specific recombination: a defined in
RT   vitro system";
RL   Cell 25:713-719(1981).
XX
RN   [3]
RC   pSVAT1 from pBR322 & SV40, small t gene
RC   pPLcSVt5 from pSVAT1 & pPLc28, lambda pL promoter
RC   pPLcSVt5-37, pPLcSVt5-77 from pPLcSVt5
RA   Derom C., Gheysen D., Fiers W.;
RT   "High-level synthesis in Escherichia coli of the SV40 small-t
RT   antigen under control of the bacteriophage lambda pL promoter";
RL   Gene 17:45-54(1982).
XX
RN   [4]
RC   from lambda, pL promoter
RA   Bernard H.U., Helinski D.R.;
RT   "Use of the lambda phage promoter pL to promote gene expression in
RT   hybrid plasmid cloning vehicles";
RL   Meth. Enzymol. 68:482-492(1979).
XX
RN   [5]
RC   from T4
RA   Hinton D.M., Silver L.L., Nossal N.G.;
RT   "Bacteriophage T4 DNA replication protein 41 - Cloning of the gene
RT   and purification of the expressed protein";
RL   J. Biol. Chem. 260:12851-12857(1985).
XX
RN   [6]
RC   p31-356 from p356 & pKC31
RC   p39-AS from pAS1 & p39
RA   Mott J.E., Grant R.A., Ho Y.S., Platt T.;
RT   "Maximizing gene expression from plasmid vectors containing the
RT   lambda pL promoter: strategies for overproducing transcription
RT   termination factor rho";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:88-92(1985).
XX
RN   [7]
RC   lambda drho from lambda & E.coli chromosome, rho gene
RC   p7, p8 from pBR322 & lambda drho
RC   p91 from pACYC184 & lambda drho
RC   [lambda ML4 from lambda & Tn5]
RC   p39, p53, p82, p252, p279, p290 from p7 & lambda ML4, Tn5
RC   p155, p202 from p91 & lambda ML4, Tn5
RC   p318, p352, p356, p385 from p202
RC   p350 series, p360 series, p370 series, p380 series from p202
RA   Brown S., Albrechtsen B., Pedersen S., Klemm P.;
RT   "Localization and regulation of the structural gene for
RT   transcription-termination factor rho of Escherichia coli";
RL   J. Mol. Biol. 162:283-298(1982).
XX
RN   [8]
RC   pKC31 from pKC30 & linker
RA   Ferguson B.;
RT   ;
RL   Unpublished (1985).
XX
CC   gamma-delta transposon is similar to Tn3.
CC   cI857 represses at 29 but not 42 degrees.
CC   nalidixic acid induces promoter in cI+ strains.
CC   NM (pPL-lambda)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Pharmacia)
CC   HO (E.coli N99cI+)(E.coli N4830-1)
CC   CP ()
CC   FN (expression)(thermoinducible)
CC   SE ()
CC   PA (pBR322)(lambda)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda 800bp, pL promoter/cI857 gene
FT                   \ gamma-delta
FT                   2. pBR322 4361bp
FT                   -> pPL-lambda 5216bp"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda leftward operator OL"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PL; leftward"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda nut"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda N"
FT   misc_binding    472..472
FT                   /note="SIT unique HpaI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda IL1"
FT   misc_binding    1232..1232
FT                   /note="SIT BamHI, at boundary of pBR322 & lambda"
FT   misc_binding    3074..3074
FT                   /note="SIT unique Tth111I"
FT   rep_origin      complement(0..3330)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    complement(3330..3330)
FT                   /note="SIT unique AflIII"
FT   CDS             complement(0..5025)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    4464..4464
FT                   /note="SIT unique PstI"
FT   misc_binding    5216..5216
FT                   /note="SIT unique EcoRI"
XX
SQ   Sequence 5216 BP; 1299 A; 1388 C; 1303 G; 1226 T; 0 other;
     aattcccggg gatccccggg gatctctcac ctaccaaaca atgcccccct gcaaaaaata
     aattcatata aaaaacatac agataaccat ctgcggtgat aaattatctc tggcggtgtt
     gacataaata ccactggcgg tgatactgag cacatcagca ggacgcactg accaccatga
     aggtgacgct cttaaaaatt aagccctgaa gaagggcagc attcaaagca gaaggctttg
     gggtgtgtga tacgaaacga agcattggcc gtaagtgcga ttccggatta gctgccaatg
     tgccaatcgc ggggggtttt cgttcaggac tacaactgcc acacaccacc aaagctaact
     gacaggagaa tccagatgga tgcacaaaca cgccgccgcg aacgtcgcgc agagaaacag
     gctcaatgga aagcagcaaa tcccctgttg gttggggtaa gcgcaaaacc agttaaccgc
     cctattctct cgctgaatcg caaaccgaaa tcacgagtag aaagcgcact aaatccgata
     gaccttacag tgctggctga ataccacaaa cagattgaaa gcaacctgca acgtattgag
     cgcaagaatc agcgcacatg gtacagcaag cctggcgaac gcggcataac atgcagtgga
     cgccagaaaa ttaagggaaa atcgattcct cttatctagt tacttagata ttggccttgg
     ctttatctca atattatatg gatcatagct ggcaactaat tcagtccagt aaatatcctc
     aatagggaat aatatatgct ttccattcca tcgggaaaaa gttttgttca acacaccaag
     ctcaatcaac tcactaatgt atgggaattg ttttgatgta accacatact tcctgccttc
     attaagggct gcgcacaaaa ccatagattg ctcttctgta aggttttgaa ttactgatcg
     cactttatcg ttttgcatct taatgcgttt tcttagctta aatcgcttat atctggcgct
     ggcaatagct gataatcgat gcacattaat tgctagcgaa aatgcaagag caaagacgaa
     aacatgccac acatgaggaa taccgattct ctcattaaca tattcaggcc agttatctgg
     gcttaaaagc agaagtccaa cccagataac gatcatatac atggttctct ccagaggttc
     attactgaac actcgtccga gaataacgag tggatcctct acgccggacg catcgtggcc
     ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
     gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta
     acttcgatca ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg
     aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt
     cgcggtgcat ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg
     gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa
     ccaacccttg gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca
     tctcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga
     cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc
     gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct
     tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt
     tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa
     cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca
     gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg
     tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca
     cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa
     gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca
     tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg
     gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
     gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
     ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaag
//