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ID   PPOLYI     preliminary; circular DNA; SYN; 2048 BP.
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AC   M18125; M18126; M18127;
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DT   16-JUL-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pPolyI - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-73
RC   ptg190 from pML2 & pUC8
RC   ptg191 from ptg190
RC   pPolyI or ptg1poly from ptg191 & M13tg131
RC   pPolyI+ from pPolyI & linker
RC   pPolyI+B0 from pPolyI+ & linker
RC   pPolyII from pPolyI+B0 & oligo
RC   M13-sn-19 from M13mp18 & oligo
RC   pPolyII-sn-14 from M13-sn-19 & pPolyII
RC   pPolyIII from pPolyII & M13-sn-19
RC   pPolyIII-I, pPolyIII-D from pPolyII-sn-14 & M13-sn-19
RC   pPolyIII-I-AMP, pPolyIII-I-ORI from M13tg119 & pPolyIII-I
RC   pPolyIII-D-AMP, pPolyIII-D-ORI from M13tg119 & pPolyIII-D
RC   EMBL301 or ABRO1, EMBL302 from EMBL3 & M13-sn-19
RA   Lathe R., Vilotte J.L., Clark A.J.;
RT   "Plasmid and bacteriophage vectors for excision of intact
RT   inserts";
RL   Gene 57:193-201(1987).
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RN   [2]
RP   1-2048
RC   pPolyI; revises [1]
RA   Lathe R.;
RT   ;
RL   Submitted (26-SEP-1988) to GenBank on computer-readable media by:
RL   Lathe R., LGME-CNRS & U184 INSERM 67085 Strasbourg, France.
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CC   NM (pPolyI)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pML2)
CC   BR (pPolyII)(pPolyIII-I)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pML2 remove DraI/AhaIII-DraI/AhaIII 711bp
FT                   \ 1772..2483, amp gene with no PstI/2189bp
FT                   2. pUC8 DraI/AhaIII-DraI/AhaIII 711bp
FT                   \ 1074..1785, amp gene with PstI
FT                   -> ptg190 2900bp
FT                   1. ptg190 NruI 2900bp 905..905
FT                   BglII linker 8bp cagatctg
FT                   -> ptg191 2900bp
FT                   1. ptg191 remove EcoRI-BglII/NruI 905bp 0..905, 1981bp
FT                   2. M13tg131 BglII-EcoRI 67bp 6234..6301, MCS
FT                   -> pPolyI 2048bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 2048 BP; 509 A; 527 C; 493 G; 519 T; 0 other;
     gaattcccgg gagagctcga tatcgcatgc ggtacctcta gaagaagctt gggatccgtc
     gacctgcaga tctgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcacggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaatcaat ctaaagtata
     tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     tcgccagtta atagtttgcg caacgttgtt gccattgtgc aggcatcgtg gtgtcacgct
     cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     gtcttcaa
//