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ID   PPOLYII    preliminary; circular DNA; SYN; 2061 BP.
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AC   M18128; M18129; M18130;
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DT   16-JUL-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pPolyII - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-109
RC   ptg190 from pML2 & pUC8
RC   ptg191 from ptg190
RC   pPolyI or ptg1poly from ptg191 & M13tg131
RC   pPolyI+ from pPolyI & linker
RC   pPolyI+B0 from pPolyI+ & linker
RC   pPolyII from pPolyI+B0 & oligo
RC   M13-sn-19 from M13mp18 & oligo
RC   pPolyII-sn-14 from M13-sn-19 & pPolyII
RC   pPolyIII from pPolyII & M13-sn-19
RC   pPolyIII-I, pPolyIII-D from pPolyII-sn-14 & M13-sn-19
RC   pPolyIII-I-AMP, pPolyIII-I-ORI from M13tg119 & pPolyIII-I
RC   pPolyIII-D-AMP, pPolyIII-D-ORI from M13tg119 & pPolyIII-D
RC   EMBL301 or ABRO1, EMBL302 from EMBL3 & M13-sn-19
RA   Lathe R., Vilotte J.L., Clark A.J.;
RT   "Plasmid and bacteriophage vectors for excision of intact
RT   inserts";
RL   Gene 57:193-201(1987).
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RN   [2]
RP   1-2061
RC   pPolyII; revises [1]
RA   Lathe R.;
RT   ;
RL   Submitted (26-SEP-1988) to GenBank on computer-readable media by:
RL   Lathe R., LGME-CNRS & U184 INSERM 67085 Strasbourg, France.
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CC   NM (pPolyII)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pML2)
CC   BR (pPolyI)(pPolyIII-I)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pPolyI SalI-BamHI 2042bp 59..2048..53
FT                   Klenow
FT                   PvuII linker 8bp ccagctgg
FT                   -> pPolyI+ 2050bp
FT                   1. pPolyI+ BamHI 2050bp
FT                   S1 nuclease [makes BalI]
FT                   -> pPolyI+B0 2050bp
FT                   1. pPolyI+B0 EcoRI 2050bp 2..2
FT                   2. oligo 11bp aattcggatcc
FT                   -> pPolyII 2061bp [BamHI-EcoRI]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 2061 BP; 512 A; 530 C; 497 G; 522 T; 0 other;
     ggatccgaat tcccgggaga gctcgatatc gcatgcggta cctctagaag aagcttggcc
     agctggtcga cctgcagatc tgcgacgcga ggctggatgg ccttccccat tatgattctt
     ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     gacgaccatc agggacagct tcacggccag caaaaggcca ggaaccgtaa aaaggccgcg
     ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaatcaatct
     aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     taagtagttc gccagttaat agtttgcgca acgttgttgc cattgtgcag gcatcgtggt
     gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     gccctttcgt cttcaagaat t
//