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ID   PPUR       preliminary; circular DNA; SYN; 4257 BP.
XX
AC   U07648;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pPUR - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4257
RC   pPUR from pBR322
RA   Kitts P.A.;
RT   ;
RL   Unpublished (1994).
XX
RN   [2]
RP   1-4257
RC   pBSpac, pBSpacdeltap from pBSV9 & pVN3.1, pac gene
RC   pSV2pacdeltap from pSV2-beta-G & pVN3.1, pac gene
RA   De La Luna S., Soria I., Pulido D., Ortin J., Jimenez A.;
RT   "Efficient transformation of mammalian cells with constructs
RT   containing a puromycin-resistance marker";
RL   Gene 62:121-126(1988).
XX
RN   [3]
RP   1-4257
RC   pPUR
RA   Kitts P.A.;
RT   ;
RL   Submitted (11-MAR-1994) by:
RL   Kitts P.A., CLONTECH Laboratories, Inc.,
RL   4030 Fabian Way, Palo Alto, CA 94303, USA.
XX
RN   [4]
RC   pBSV9 from pBR322 & SV40
RA   Portela A., Melero J.A., Martinez C., Domingo E., Ortin J.;
RT   "Oriented synthesis and cloning of influenza virus nucleoprotein
RT   cDNA that leads to its expression in mammalian cells";
RL   Virus Res. 4:69-82(1985).
XX
RN   [5]
RC   pVN3.1 from pac gene
RA   Vara J., Portela A., Ortin J., Jimenez A.;
RT   "Expression in mammalian cells of a gene from Streptomyces alboniger
RT   conferring puromycin resistance";
RL   Nucleic Acids Res. 14:4617-4624(1986).
XX
CC   NCBI gi: 470667
CC   NM (pPUR)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)(fusion)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   -> pPUR 4257bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 4257 BP; 1023 A; 1178 C; 1044 G; 1012 T; 0 other;
     ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttgcatg cctgcaggtc
     ggccgccacg accggtgccg ccaccatccc ctgacccacg cccctgaccc ctcacaagga
     gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     ccgtcaccgc cgacgtcgag tgcccgaagg accgcgcgac ctggtgcatg acccgcaagc
     ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     atggctccga ccgaagccga cccgggcggc cccgccgacc ccgcacccgc ccccgaggcc
     caccgactct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     tatcatgtct ggatccccag gaagctcctc tgtgtcctca taaaccctaa cctcctctac
     ttgagaggac attccaatca taggctgccc atccaccctc tgtgtcctcc tgttaattag
     gtcacttaac aaaaaggaaa ttgggtaggg gtttttcaca gaccgctttc taagggtaat
     tttaaaatat ctgggaagtc ccttccactg ctgtgttcca gaagtgttgg taaacagccc
     acaaatgtca acagcagaaa catacaagct gtcagctttg cacaagggcc caacaccctg
     ctcatcaaga agcactgtgg ttgctgtgtt agtaatgtgc aaaacaggag gcacattttc
     cccacctgtg taggttccaa aatatctagt gttttcattt ttacttggat caggaaccca
     gcactccact ggataagcat tatccttatc caaaacagcc ttgtggtcag tgttcatctg
     ctgactgtca actgtagcat tttttggggt tacagtttga gcaggatatt tggtcctgta
     gtttgctaac acaccctgca gctccaaagg ttccccacca acagcaaaaa aatgaaaatt
     tgacccttga atgggttttc cagcaccatt ttcatgagtt ttttgtgtcc ctgaatgcaa
     gtttaacata gcagttaccc caataacctc agttttaaca gtaacagctt cccacatcaa
     aatatttcca caggttaagt cctcatttaa attaggcaaa ggaattcttg aagacgaaag
     ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccag
//