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ID PRAJ275 preliminary; circular DNA; SYN; 4519 BP.
XX
AC U02456;
XX
DT 03-FEB-1994 (Rel. 8, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli plasmid vector pRAJ275 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-4519
RC pRAJ275
RA Kitts P.A.;
RT "CLONTECH Vectors On Disc version 1.1";
RL Unpublished (1993).
XX
RN [2]
RP 1-4519
RC pRAJ275
RA Kitts P.A.;
RT ;
RL Submitted (07-OCT-1993) by:
RL Kitts P.A., CLONTECH Laboratories, Inc.,
RL 4030 Fabian Way, Palo Alto, CA 94303, USA.
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RN [3]
RC pBI101.1, pBI111.1, pBI121.1, pBI131 from pBIN19
RA Jefferson R.A.;
RT "Plant reporter genes: the GUS gene fusion system";
RL Genet. Eng. 10:247-263(1988).
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RN [4]
RC p35S-7S from pMON505 & beta-conglycinin alpha 3' & CaMV 35S promoter
RC plasmid from pRAJ275 & p35S-7S
RC plasmid2 from plasmid & pBluescript KS+
RC NOTE: GUS=beta-glucuronidase
RA Farrell L.B., Beachy R.N.;
RT "Manipulation of beta-glucuronidase for use as a reporter in
RT vacuolar targeting studies";
RL Plant Mol. Biol. 15:821-825(1990).
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RN [5]
RC pMON503 from pMON200
RC pMON504 from pMON8015
RC pMON505 from pMON503 & pTJS75
RC pMON506 from pMON504 & pTJS75
RC pMON507a, pMON507b from pMON505 & pTiA6
RC pMON508, pMON508W from pMON506 & pTiA6
RA Horsch R., Klee H.;
RT "Rapid assay of foreign gene expression in leaf discs transformed by
RT Agrobacterium tumefaciens: role of T-DNA borders in the transfer
RT process";
RL Proc. Natl. Acad. Sci. U.S.A. 83:4428-4432(1986).
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RN [6]
RC plasmid from pUC19 & oligo, translation initiator
RC pRAJ275 from pRAJ255 & plasmid
RC pBI221.1 from pBI121 & pUC19
RC pBI121.2 from pRAJ275 & pBIN19
RC pBI221.2 from pRAJ275 & pUC19
RA Jefferson R.A.;
RT "Assaying chimeric genes in plants: the GUS gene fusion system";
RL Plant Mol. Biol. Rep. 5:387-405(1987).
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RN [7]
RC pRAJ210, pRAJ220 from pUC9 & pBKuidA
RC pRAJ230 from pRAJ220 & pUC9
RC pRAJ240 from pRAJ230 & linker
RC pRAJ250 from pRAJ230 & pEMBL9
RC pRAJ255 from pRAJ250
RC pRAJ260 from pRAJ255 & pEMBL9 & linker
RA Jefferson R.A., Burgess S.M., Hirsch D.;
RT "beta-Glucuronidase from Escherichia coli as a gene-fusion marker";
RL Proc. Natl. Acad. Sci. U.S.A. 83:8447-8451(1986).
XX
RN [8]
RC pBKuidA from pBR325 & E.coli uidA-manA genes
RA Bitner R., Kuempel P.;
RT ;
RL Unpublished (1986).
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CC This sequence has been compiled from information in the sequence
CC databases, published literature and other sources; this vector has
CC not been completely sequenced. This vector is no longer available
CC from CLONTECH and CLONTECH will not update or revise this
CC sequence.
CC NCBI gi: 413822
CC old CLONTECH vector.
CC NM (pRAJ275)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)(yeast)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (pBR322)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pEMBL9+ remove PstI-EcoRI 18bp 241..259,
FT \ MCS/3921bp
FT 2. E. coli PstI-EcoRI 1870bp, GUS gene
FT -> pRAJ260
FT 1. pBR325 remove EcoRI-HindIII 1246bp 4778..5994..30,
FT \ 4750bp
FT 2. E.coli EcoRI-HindIII, uidA-manA genes
FT -> pBKuidA
FT 1. pUC9 remove HindIII-EcoRI 30bp 234..264,MCS/2635bp
FT 2. pBKuidA EcoRI-HindIII, uidA-manA genes
FT -> pRAJ210
FT 1. pRAJ210 XhoI
FT BAL31 nuclease
FT -> pRAJ220 [2439bp uidA-manA genes]
FT 1. pRAJ220 HinfI-BamHI 515bp, 5' to ATG to GUS gene
FT fill in
FT 2. pUC9 remove HincII-BamHI 4bp 250..254, MCS/2661bp
FT -> plasmid 3176bp
FT 1. plasmid BamHI 3176bp
FT 2. pRAJ220 BamHI-BamHI 1600bp, 3' uidA gene
FT -> pRAJ230 4800bp
FT 1. pRAJ230 AatII-PstI 1860bp, 45bp 3' to uidA term
FT fill in:
FT 2. pEMBL9+ remove PstI-SmaI 15bp 241..256, MCS/3924bp
FT -> pRAJ250 5780bp
FT 1. pRAJ250 BamHI 5780bp 807..807, in uidA gene
FT mutagenesis
FT -> pRAJ255 5780bp
FT 1. pUC19 remove EcoRI-SalI 33bp 397..430, MCS/2653bp
FT 2. oligo SalI-EcoRI 13bp
FT \ gtcgaccatggtc, Kozak translation initiator
FT -> plasmid 2666bp
FT 1. pRAJ255 5780bp, 5' GUS
FT BAL31 nuclease
FT 2. plasmid SalI-EcoRI 13bp, Kozak translation init
FT -> pRAJ275 4519bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ Sequence 4519 BP; 1130 A; 1131 C; 1190 G; 1068 T; 0 other;
aagcttgcat gcctgcaggt cgaccatggt ccgtcctgta gaaaccccaa cccgtgaaat
caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg gaattgatca
gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag gcagttttaa
cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt atcagcgcga
agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg atgcggtcac
tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg gcggctatac
gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac gtatcaccgt
ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga ttaccgacga
aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg gaatccatcg
cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg tggtgacgca
tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca atggtgatgt
cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag gcactagcgg
gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc tctatgaact
gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgcttcgcg tcggcatccg
gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct actttactgg
ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg tgctgatggt
gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct cgcattaccc
ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga ttgatgaaac
tgctgctgtc ggctttaacc tctctttagg cattggtttc gaagcgggca acaagccgaa
agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact tacaggcgat
taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga gtattgccaa
cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg cggaagcaac
gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct gcgacgctca
caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt acggatggta
tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac ttctggcctg
gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata cgttagccgg
gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat ggctggatat
gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat ggaatttcgc
cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag ggatcttcac
tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga ctggcatgaa
cttcggtgaa aaaccgcagc agggaggcaa acaatgaatc aacaactctc ctggcgcacc
atcgtcggct acagcctcgg tggggaattc actggccgtc gttttacaac gtcgtgactg
ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
ctatgaccat gattacgcc
//