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ID   PRAJ275    preliminary; circular DNA; SYN; 4519 BP.
XX
AC   U02456;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pRAJ275 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4519
RC   pRAJ275
RA   Kitts P.A.;
RT   "CLONTECH Vectors On Disc version 1.1";
RL   Unpublished (1993).
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RN   [2]
RP   1-4519
RC   pRAJ275
RA   Kitts P.A.;
RT   ;
RL   Submitted (07-OCT-1993) by:
RL   Kitts P.A., CLONTECH Laboratories, Inc.,
RL   4030 Fabian Way, Palo Alto, CA 94303, USA.
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RN   [3]
RC   pBI101.1, pBI111.1, pBI121.1, pBI131 from pBIN19
RA   Jefferson R.A.;
RT   "Plant reporter genes: the GUS gene fusion system";
RL   Genet. Eng. 10:247-263(1988).
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RN   [4]
RC   p35S-7S from pMON505 & beta-conglycinin alpha 3' & CaMV 35S promoter
RC   plasmid from pRAJ275 & p35S-7S
RC   plasmid2 from plasmid & pBluescript KS+
RC   NOTE: GUS=beta-glucuronidase
RA   Farrell L.B., Beachy R.N.;
RT   "Manipulation of beta-glucuronidase for use as a reporter in
RT   vacuolar targeting studies";
RL   Plant Mol. Biol. 15:821-825(1990).
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RN   [5]
RC   pMON503 from pMON200
RC   pMON504 from pMON8015
RC   pMON505 from pMON503 & pTJS75
RC   pMON506 from pMON504 & pTJS75
RC   pMON507a, pMON507b from pMON505 & pTiA6
RC   pMON508, pMON508W from pMON506 & pTiA6
RA   Horsch R., Klee H.;
RT   "Rapid assay of foreign gene expression in leaf discs transformed by
RT   Agrobacterium tumefaciens: role of T-DNA borders in the transfer
RT   process";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:4428-4432(1986).
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RN   [6]
RC   plasmid from pUC19 & oligo, translation initiator
RC   pRAJ275 from pRAJ255 & plasmid
RC   pBI221.1 from pBI121 & pUC19
RC   pBI121.2 from pRAJ275 & pBIN19
RC   pBI221.2 from pRAJ275 & pUC19
RA   Jefferson R.A.;
RT   "Assaying chimeric genes in plants: the GUS gene fusion system";
RL   Plant Mol. Biol. Rep. 5:387-405(1987).
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RN   [7]
RC   pRAJ210, pRAJ220 from pUC9 & pBKuidA
RC   pRAJ230 from pRAJ220 & pUC9
RC   pRAJ240 from pRAJ230 & linker
RC   pRAJ250 from pRAJ230 & pEMBL9
RC   pRAJ255 from pRAJ250
RC   pRAJ260 from pRAJ255 & pEMBL9 & linker
RA   Jefferson R.A., Burgess S.M., Hirsch D.;
RT   "beta-Glucuronidase from Escherichia coli as a gene-fusion marker";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:8447-8451(1986).
XX
RN   [8]
RC   pBKuidA from pBR325 & E.coli uidA-manA genes
RA   Bitner R., Kuempel P.;
RT   ;
RL   Unpublished (1986).
XX
CC   This sequence has been compiled from information in the sequence
CC   databases, published literature and other sources; this vector has
CC   not been completely sequenced. This vector is no longer available
CC   from CLONTECH and CLONTECH will not update or revise this
CC   sequence.
CC   NCBI gi: 413822
CC   old CLONTECH vector.
CC   NM (pRAJ275)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(yeast)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pEMBL9+ remove PstI-EcoRI 18bp 241..259,
FT                   \ MCS/3921bp
FT                   2. E. coli PstI-EcoRI 1870bp, GUS gene
FT                   -> pRAJ260
FT                   1. pBR325 remove EcoRI-HindIII 1246bp 4778..5994..30,
FT                   \ 4750bp
FT                   2. E.coli EcoRI-HindIII, uidA-manA genes
FT                   -> pBKuidA
FT                   1. pUC9 remove HindIII-EcoRI 30bp 234..264,MCS/2635bp
FT                   2. pBKuidA EcoRI-HindIII, uidA-manA genes
FT                   -> pRAJ210
FT                   1. pRAJ210 XhoI
FT                   BAL31 nuclease
FT                   -> pRAJ220 [2439bp uidA-manA genes]
FT                   1. pRAJ220 HinfI-BamHI 515bp, 5' to ATG to GUS gene
FT                   fill in
FT                   2. pUC9 remove HincII-BamHI 4bp 250..254, MCS/2661bp
FT                   -> plasmid 3176bp
FT                   1. plasmid BamHI 3176bp
FT                   2. pRAJ220 BamHI-BamHI 1600bp, 3' uidA gene
FT                   -> pRAJ230 4800bp
FT                   1. pRAJ230 AatII-PstI 1860bp, 45bp 3' to uidA term
FT                   fill in:
FT                   2. pEMBL9+ remove PstI-SmaI 15bp 241..256, MCS/3924bp
FT                   -> pRAJ250 5780bp
FT                   1. pRAJ250 BamHI 5780bp 807..807, in uidA gene
FT                   mutagenesis
FT                   -> pRAJ255 5780bp
FT                   1. pUC19 remove EcoRI-SalI 33bp 397..430, MCS/2653bp
FT                   2. oligo SalI-EcoRI 13bp
FT                   \ gtcgaccatggtc, Kozak translation initiator
FT                   -> plasmid 2666bp
FT                   1. pRAJ255 5780bp, 5' GUS
FT                   BAL31 nuclease
FT                   2. plasmid SalI-EcoRI 13bp, Kozak translation init
FT                   -> pRAJ275 4519bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 4519 BP; 1130 A; 1131 C; 1190 G; 1068 T; 0 other;
     aagcttgcat gcctgcaggt cgaccatggt ccgtcctgta gaaaccccaa cccgtgaaat
     caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg gaattgatca
     gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag gcagttttaa
     cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt atcagcgcga
     agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg atgcggtcac
     tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg gcggctatac
     gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac gtatcaccgt
     ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga ttaccgacga
     aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg gaatccatcg
     cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg tggtgacgca
     tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca atggtgatgt
     cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag gcactagcgg
     gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc tctatgaact
     gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgcttcgcg tcggcatccg
     gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct actttactgg
     ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg tgctgatggt
     gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct cgcattaccc
     ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga ttgatgaaac
     tgctgctgtc ggctttaacc tctctttagg cattggtttc gaagcgggca acaagccgaa
     agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact tacaggcgat
     taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga gtattgccaa
     cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg cggaagcaac
     gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct gcgacgctca
     caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt acggatggta
     tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac ttctggcctg
     gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata cgttagccgg
     gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat ggctggatat
     gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat ggaatttcgc
     cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag ggatcttcac
     tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga ctggcatgaa
     cttcggtgaa aaaccgcagc agggaggcaa acaatgaatc aacaactctc ctggcgcacc
     atcgtcggct acagcctcgg tggggaattc actggccgtc gttttacaac gtcgtgactg
     ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     ctatgaccat gattacgcc
//