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ID   PRBK       preliminary; circular DNA; SYN; 10173 BP.
XX
AC   IG1102;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pRBK - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRcRSV from RSV
RC   pECE from SV40
RC   pSK+ from pUC series
RA   Kung H.J., Boerkoel C., Carter T.H.;
RT   "Retroviral mutagenesis of cellular oncogenes: a review with
RT   insights into the mechanisms of insertional activation";
RL   Curr. Top. Microbiol. Immunol. 171:1-25(1991).
XX
CC   NM (pRBK)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli)(mammal)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. RSV, LTR
FT                   3. SV40, early polyA/tk gene
FT                   4. E. coli 1142bp, hyg gene
FT                   5. BK, T antigen
FT                   -> pRBK 10173bp"
FT   promoter        0..0
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   misc_binding    0..0
FT                   /note="MCS NheI-NotI-XhoI-SfiI-BamHI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    802..802
FT                   /note="SIT HpaI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 thymidine kinase (TK) gene"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli hygromycin phosphotransferase gene;
FT                   hygromycin resistance gene (hyg)"
FT   misc_binding    1818..1818
FT                   /note="SIT SacII"
FT   promoter        complement(0..0)
FT                   /note="PRO SV40 thymidine kinase (TK) gene"
FT   misc_binding    2743..2743
FT                   /note="SIT BstBI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    5868..5868
FT                   /note="SIT BglII"
FT   CDS             complement(0..0)
FT                   /note="GEN human papova virus (BK) T antigen gene"
FT   rep_origin      0..0
FT                   /note="ORI human papova virus (BK)"
FT   misc_binding    9740..9740
FT                   /note="SIT BalI"
XX
SQ   Sequence 10173 BP; 2676 A; 2395 C; 2377 G; 2725 T; 0 other;
     gtcgaccaat tctcatgttt gacagcttat catcgcagat cctgagcttg tatggtgcac
     tctcagtaca atctgctctg ctgccgcata gttaagccag tatctgctcc ctgcttgtgt
     gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     ggccagatat acgcgtatct gaggggacta gggtgtgttt aggcgcccag cggggcttcg
     gttgtacgcg gttaggagtc ccctcaggat atagtagttt cgcttttgca tagggagggg
     gaaatgtagt cttatgcaat acacttgtag tcttgcaaca tggtaacgat gagttagcaa
     catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     gatcgtgcct tattaggaag gcaacagaca ggtctgacat ggattggacg aaccactgaa
     ttccgcattg cagagataat tgtatttaag tgcctagctc gatacaataa acgccatttg
     accattcacc acattggtgt gcacctccaa gctgggtacc agctgctagc aagcttgcta
     gcggccgctc gaggccggca aggccggatc cagacatgat aagatacatt gatgagtttg
     gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     attttatgtt tcaggttcag ggggaggtgg ggaggttttt taaagcaagt aaaacctcta
     caaatgtggt atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa
     aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     accggtcgac caagcttggc actgggcgcc agaaatccgc gcggtggttt ttgggggtcg
     ggggtgtttg gcagccacag acgcccggtg ttcgtgtcgc gccagtacat gcggtccatg
     cccaggccat ccaaaaacca tgggtctgtc tgctcagtcc agtcgtggac cagaccccac
     gcaacgccca aaataataac ccccacgaac cataaaccat tccccatggg ggaccccgtc
     cctaacccac ggggccagtg gctatggcag ggcctgccgc cccgacgttg gctgcgagcc
     ctgggccttc acccgaactt ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt
     ggccccaatg gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc
     gtttatgaac aaacgaccca acacccgtgc gttttattct gtctttttat tgccgtcata
     gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc ccatctcccc
     tattcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct
     acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc
     ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa
     ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg
     agccgcggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc
     atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg
     aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg
     ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc
     atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc
     cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga
     ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg
     atcgcatcca tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg
     tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat
     tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca
     taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct
     cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag
     acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt
     ttcatatctc attgcccggg atctgcggca cgctgttgac gctgttaagc gggtcgctgc
     agggtcgctc ggtgttcgag gccacacgcg tcaccttaat atgcgaagtg gacctgggac
     cgcgccgccc cgactgcatc tgcgtgttcg aattcgccaa tgacaagacg ctgggcgggg
     tttgtgtcat catagaacta aagacatgca aatatatttc ttccggggac accgccagca
     aacgcgagca acgggccacg gggatgaagc agggcatggc ggccgacgcg ctgggctacg
     tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcactggac
     cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga
     ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc
     gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag
     aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa
     catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcagcaaaa ggccaggaac
     cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattctca
     tgtttgacag cttatctcta gcagatccgg aattcccctc cccaatttaa atgaggacct
     aacctgtgga aatctactga tgtgggaggc tgtaactgta caaacagagg ttattggaat
     aactagcatg cttaaccttc atgcagggtc acaaaaagtg catgacgatg gtggaggaaa
     acctattcaa ggcagtaatt tccacttctt tgctgttggt ggagacccct tggaaatgca
     gggagtgcta atgaattaca ggacaaagta cccagatggt actataaccc ctaaaaaccc
     aacagcccag tcccaggtaa tgaatactga ccataaggcc tatttggaca aaaacaatgc
     ttatccagtt gagtgctggg ttcctgatcc tagtagaaat gaaaatacta ggtattttgg
     gactttcaca ggaggggaaa atgttccccc agtacttcat gtgaccaaca cagctaccac
     agtgttgcta gatgaacagg gtgtggggcc tctttgtaaa gctgatagcc tgtatgtttc
     agctgctgat atttgtggcc tgtttactaa cagctctgga acacaacagt ggagaggcct
     tgcaagatat tttaagatcc gcctgagaaa aagatctgta aagaatcctt acctaatttc
     ctttttgcta agtgacctta taaacaggag aacccagaga gtggatgggc agcctatgta
     tggtatggaa tcccaggtag aagaggttag ggtgtttgat ggcacagaaa gacttccagg
     ggacccagat atgataagat atattgacaa acagggacaa ttgcaaacca aaatgcttta
     aacaggtgct tttattgtac atatacattt aataaatgct gcttttgtat aagccacttt
     taagcttgtg ttattttggg ggtggtgttt taggcctttt aaaacactga aagcctttac
     acaaatgcaa ctcttgacta tgggggtctg acctttggga atgttcagca ggggctgaag
     tatctgagac ttgggaagag cattgtgatt gggattcagt gcttgatcca tgtccagagt
     cttcagtttc tgaatcctct tctcttgtaa tatcaagaat acatttcccc atgcatatat
     tatatttcat ccttgaaaaa gtatacatac ttatctcaga atccagcctt tccttccatt
     caacaattct agattgtata tcagttgcaa aatcagctac aggcctaaac caaattagca
     gtagcaacaa ggtcattcca ctttgtaaaa ttcttttttc aagtaagaac tctgagtttt
     gtaaggattt tcttaaatat attttgggcc taaaatctat ttgtcttaca aatctagctt
     gcagggtttt agggacagga tactcattca ttgtaaccaa gcctggtgga aatatttggg
     ttcttttgtt taaatgtttc ttttctaaat ttaccttaac acttccatct aaataatctc
     tcaaactgtc taaattgttt attccatgtc ctgaaggcaa atcctttgat tcagctcctg
     tcccttttac atcttcaaaa acaaccatgt actgatctat agctacacct agctcaaagg
     ttagcctttc catgggtagg tttacattta aggctttacc tctacacaaa tctaacaacc
     ctgcagctag tgttgttttt ccactatcaa tgggaccttt aaataaccag tatcttcttt
     taggtacatt gaaaacaata cagtgcaaaa aatcaaatat tacagaatcc attttaggta
     gcaaacagtg cagccaagca acacctgcca tatattgttc tagtacagca tttccatgag
     ctccaaatat taaatccatt ttatctaata tatgattgaa tctttctgtt agcatttctt
     ccctgttcat atgaagggta tctactattt ttttagctaa aactgtatct actgcttgct
     gacaaataac ttttttgttt ttactttctg caaaaataat agcatttgca aagtgctttt
     catgatactt aaagtgataa ggctggtctt ttttctgaca ctttttacac tcctctacat
     tgtattgaaa ttctaaatac atacctaata ataaaaacac atcctcacac tttgtctcta
     ctgcatactc agtaattaat ttccaagaca cctgctttgt ttcttcaggc tcttctgggt
     taaaatcatg ctcctttaag cccccttgaa tgctttcttc tatagtatgg tatggatctc
     tagttaaggc actatatagt aagtattcct tattaacacc cttacaaatt aaaaaactaa
     aggtacacag cttttgacag aaattattaa ttgcagaaac tctatgtcta tgtggagtta
     aaaagaatat aatattatgc ccagcacaca tgtgtctact aataaaagtt acagaatatt
     tttccataag ttttttatac agaatttgag ctttttcttt agtagtatac acagcaaagc
     aggcaagggt tctattacta aatacagctt gactaagaaa ctggtgtaga tcagagggaa
     agtctttagg gtcttctacc tttctttttt ttttgggtgg tgttgagtgt tgagaatctg
     ctgttgcttc ttcatcactg gcaaacatat cttcatggca aaataaatct tcatcccatt
     tttcattaaa ggaactccac caggactccc actcttctgt tccataggtg ggcaccaata
     aaaaaaataa ttacttaggg cataggccat tccttgcagt acagggtatc tgggcaaaga
     ggaaaatcag cacaaacctc tgagctactc caggttccaa aatcaggctg atgagctacc
     tttacatcct gctccatttt tttatacaaa gtattcattc tcttcatttt atcctcgtcg
     ccccctttgt cagggtgaaa ttccttacac ttccttaaag cttttctcat taagggaaga
     tttccccagg cagctctttc aaggcctaaa aggtccatga gctccatgga ttcttccctg
     ttaagaactt tatccatttt tgcaaaaatt gcaaaagaat agggatttcc ccaaatagtt
     ttgctaggcc tcagaaaaag cctccacacc cttactactt gagagaaagg gtggaggcag
     aggcggcctc ggcctcttat atattataaa aaaaaaggcc acagggagga gctgcttacc
     catggaatgc agccaaacca tgacctcagc aaggaaagtg catgactggg cagccagcca
     gtggcagtta atagtgaaac cccgccccta aaatctctct tacccatgga atgcagccaa
     accatgacct caggaaggaa agtgcatgac tgggcagcca gccagtggca gttaatagtg
     aaaccatgcc aaaccatgac ctcaggaagg aaagtgcatg actgggcagc cagccattgg
     cagttaattt gcgagcctag gaatcttggc cttgtcccca gttaaactgg acaaaggcca
     tggttctgcg ccagctgtca cgacaagctt cagtgaaagt tggtaaaacc tggactggaa
     caaaaaaaag agctcagagg atttttattt ttattttaga gcttttgctg gaattttgta
     gaggtgaaga cagtgtagac gggaaaaaca aaagtaccac tgctttacct gctgtaaaag
     actctgtaaa agactcctag gtaagtaatc cctttttttt tgtatttcca ggttcatggg
     tgctgctcta gcacttttgg gggacctagt tgccagtgta tctgaggctg ctgctgccac
     aggattttca gtggctgaaa ttgctgctgg ggaggctgct gctgctatag aagttcaaat
     tgcatccctt gctactgtag agggcataac aagtacctca gaggctatag ctgctatagg
     cctaactcct caaacatatg ctgtaagctt tggtgctcct ggggctattg ctgggtttgc
     tgctttaatt caaactgtta gtggtattag ttccttggct caagtagggt ataggttctt
     tagtgattgg gatcacaaag tttccactgt aggcctctat cagcaatcag gcatggcttt
     ggaattgttt aacccagatg agtactatga tattctgttt cctggtgtaa atacttttgt
     taataatatt caataccttg atcctaggca ttggggtcct tctttgtttg ctactatttg
     ggaggctttg tggcatgtta ttagggatga tataccttct ataacctcac aggaattgca
     gagaagaaca gaaagatttt ttagagactg gttggctaga tttttggagg aaactacctg
     gacaattgta aatgccccta taaactttta taattatatt caacaatatt attctgatct
     ttcccctatt aggccctcaa tggttagaca agtagctgaa agggaaggta cccgtgtaca
     ttttggccat acttatagta tagatgatgc tgacagtata gaagaagtta cacaaagaat
     ggacttaaga aatcaacaaa gtgtacattc aggagagttt atagaaaaaa tcattgcccc
     aggaggtgct aatcaaagaa ctgctcctca atggatgttg cctttacttc taggcctgta
     cgggactgta acacctgctc ttgaagcatg tgaagatggc cccaaccaaa agaaaaggag
     agtgtccagg ggcagctccc aaaaagccaa aggaacccgt gcaagtgcca aaactactaa
     taaaaggagg agtagaagtt ctagaagtta aaactggggt agatgctatt acagaggtag
     aatgcttcct aaacccagaa atgggggatc tgc
//