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ID PRC23 preliminary; circular DNA; SYN; 4638 BP.
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AC IG9873;
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DT 01-JUL-1995 (Rel. 11, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli plasmid vector pRC23 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pRC1 from pBR322 & linker
RC pRC2 from pBR322 & linker
RC pRC23 from pRC2 & oligo & lambda, pL
RC pEV-vrf1, pEV-vrf2, pEV-vrf3 from pRC23 & oligo
RA Crowl R., Seamans C., Lomedico P., McAndrew S.;
RT "Versatile expression vectors for high-level synthesis of cloned
RT gene products in Escherichia coli";
RL Gene 38:31-38(1985).
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CC Created by Moore, July 1995, under contract with NCBI.
CC NM (pRC23)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pBR322)(lambda)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 EcoRI 4361bp 4360..4360
FT S1 nuclease
FT BglII linker 8bp cagatctg
FT BglII-PstI 757bp 3612..4369
FT 2. pBR322 EcoRI 4361bp 4360..4360
FT Klenow
FT BglII linker 8bp cagatctg
FT BglII-PstI 3613bp 4360..4361..3612
FT -> pRC2 4370bp [unique BglII next to EcoRI; 4350bp]
FT 1. oligo BglII-HaeII 26bp, model RBS
FT \ agatctnnnnnnnnnnnnnnagcgct
FT 2. lambda HpaI-BglII 450bp 35262..35712, pL
FT HaeIII-BglII 244bp 35468..35712, pL
FT -> BglII-BglII 270bp, pL/RBS
FT 1. BglII-BglII 270bp, pL/RBS
FT 2. pRC2 BglII 4364bp, linker
FT -> pRC23 4640bp [unique EcoRI in SD]"
FT - 1..4359
FT /note="pBR322 1..4359 4359bp
FT EcoRI = G^AATTC
FT \ ca"
FT - 4360..4361
FT /note="ca 2bp
FT \ ca
FT \ gatct..."
FT - 4362..4386
FT /note="gatctnnnnnnnnnnnnnnagcgct 25bp
FT \ ...agcgct
FT HaeIII = GG^CC"
FT - 4387..4630
FT /note="lambda 35468..35711 244bp
FT BglII = A^GATCT"
FT - 4631..4636
FT /note="gatctg 6bp
FT \ gatctg
FT EcoRI = G^AATTC"
FT - 4637..4638
FT /note="pBR322 4360..4361 2bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ Sequence 4638 BP; 1038 A; 1268 C; 1194 G; 1124 T; 14 other;
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
ataaaaatag gcgtatcacg aggccctttc gtcttcaagc agatctnnnn nnnnnnnnnn
agcgctccaa tgcttcgttt cgtatcacac accccaaagc cttctgcttt gaatgctgcc
cttcttcagg gcttaatttt taagagcgtc accttcatgg tggtcagtgc gtcctgctga
tgtgctcagt atcaccgcca gtggtattta tgtcaacacc gccagagata atttatcacc
gcagatggtt atctgtatgt tttttatatg aatttatttt ttgcaggggg gcattgtttg
gtaggtgaga gatctgaa
//