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ID   PRCCMV     preliminary; circular DNA; SYN; 5542 BP.
XX
AC   IG1103;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pRcCMV - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRc/CMV from CMV
RC   pRc/RSV from RSV
RC   pECE from SV40
RC   pSK+ from pUC series
RA   Kung H.J., Boerkoel C., Carter T.H.;
RT   "Retroviral mutagenesis of cellular oncogenes: a review with
RT   insights into the mechanisms of insertional activation";
RL   Curr. Top. Microbiol. Immunol. 171:1-25(1991).
XX
CC   NM (pRcCMV)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(mammal)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA ()
CC   BR (pRcRSV)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. CMV, immediate early promoter
FT                   3. T7, promoter
FT                   4. Sp6, promoter
FT                   5. bGH gene polyA
FT                   6. M13, ori
FT                   7. RSV, LTR
FT                   8. Tn5, neo gene
FT                   9. SV40, early polYA
FT                   -> pRBK 5550bp
FT                   1. pRBK 5550bp
FT                   -> pRcRSV 5542bp"
FT   misc_binding    12..12
FT                   /note="SIT BglII"
FT   misc_binding    206..206
FT                   /note="SIT NruI"
FT   promoter        0..0
FT                   /note="PRO human cytomegalovirus (CMV)
FT                   immediate early gene"
FT   misc_binding    485..485
FT                   /note="SIT NdeI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-BstXI-BstXI-NotI-XbaI-ApaI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   polyA_signal    0..0
FT                   /note="PLA E. coli BGH gene"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13"
FT   promoter        0..0
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   misc_binding    2100..2100
FT                   /note="SIT SmaI"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo)"
FT   misc_binding    2404..2404
FT                   /note="SIT TthIIII"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    3209..3209
FT                   /note="SIT BsmI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    4896..4896
FT                   /note="SIT PvuI"
XX
SQ   Sequence 5542 BP; 1280 A; 1434 C; 1413 G; 1415 T; 0 other;
     gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
     ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
     atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
     ctgcttaact ggcttatcga aattaatacg actcactata gggagaccca agcttggtac
     cgagctcgga tccactagta acggccgcca gtgtgctgga attctgcaga tatccatcac
     actggcggcc gctcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct
     agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     tctatggctt ctgaggcgga aagaaccagc tggggctcga ggggggatcc ccacgcgccc
     tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta
     cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     tttaacaaaa tattaacgtt tacaatttaa atatttgctt atacaatctt cctgtttttg
     gggcttttct gattatcaac cggggtgggt accgagctcg aattctgtgg aatgtgtgtc
     agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat
     ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     tttggaggcc taggcttttg caaaaagctc ccgggagctt ggatatccat tttcggatct
     gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcccgtcga
     cctcgagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     ccgaaaagtg ccacctgacg tc
//