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ID   PRCRSV     preliminary; circular DNA; SYN; 5224 BP.
XX
AC   IG1104;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pRcRSV - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRSVADH from pWX008 & pRc/RSV
RC   pRSVPAP from pSV2Apap & pRc/RSV
RC   plasmid from pM-MuLV-SV-lacZ & pSK+
RC   plasmid from plasmid & pECE
RC   pRSVlacZ from plasmid & pRc/RSV
RC   pCMVlacZ from pM-MuLV-SV-lacZ
RC   pCMVADH from pWX008
RA   Lin W.C., Culp L.A.;
RT   "Selectable plasmid vectors with alternative and ultrasensitive
RT   histochemical marker genes";
RL   Biotechniques 11:344-351(1991).
XX
RN   [2]
RC   from pGEX series
RA   Riviere Y., Blank V., Kourilsky P., Israel A.;
RT   "Processing of the precursor of NF-kappa B by the HIV-1 protease
RT   during acute infection";
RL   Nature 350:625-626(1991).
XX
RN   [3]
RC   pRcRSV from RSV
RC   pECE from SV40
RC   pSK+ from pUC series
RA   Kung H.J., Boerkoel C., Carter T.H.;
RT   "Retroviral mutagenesis of cellular oncogenes: a review with
RT   insights into the mechanisms of insertional activation";
RL   Curr. Top. Microbiol. Immunol. 171:1-25(1991).
XX
CC   NM (pRcRSV)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(mammal)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA ()
CC   BR (pRcCMV)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. RSV
FT                   -> pRcRSV 5224bp"
FT   misc_binding    12..12
FT                   /note="SIT BglII"
FT   promoter        0..0
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SpeI-BstXI-BstXI-NotI-XbaI"
FT   polyA_signal    0..0
FT                   /note="PLA E. coli BGH gene"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   promoter        0..0
FT                   /note="PRO SV40 early gene"
FT   misc_binding    1757..1757
FT                   /note="SIT StuI"
FT   misc_binding    1781..1781
FT                   /note="SIT SmaI"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo)"
FT   misc_binding    2050..2050
FT                   /note="SIT BalI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    2651..2651
FT                   /note="SIT BstBI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    4805..4805
FT                   /note="SIT XmnI"
XX
SQ   Sequence 5224 BP; 1202 A; 1337 C; 1360 G; 1325 T; 0 other;
     gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
     ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtatctgag
     gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc
     tcaggatata gtagtttcgc ttttgcatag ggagggggaa atgtagtctt atgcaataca
     cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     acagacaggt ctgacatgga ttggacgaac cactgaattc cgcattgcag agataattgt
     atttaagtgc ctagctcgat acaataaacg ccatttgacc attcaccaca ttggtgtgca
     cctccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt gctggaattc
     tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagctcg ctgatcagcc
     tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     gaaagaacca gctggggctc gaggggggat ccccacgcgc cctgtagcgg cgcattaagc
     gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat
     tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     tttacaattt aaatatttgc ttatacaatc ttcctgtttt tggggctttt ctgattatca
     accggggtgg gtaccgagct cgaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     tgcaaaaagc tcccgggagc ttggatatcc attttcggat ctgatcaaga gacaggatga
     ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     ccaaactcat caatgtatct tatcatgtct ggatcccgtc gacctcgaga gcttggcgta
     atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     cgtc
//