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ID PREP10 preliminary; circular DNA; SYN; 8503 BP.
XX
AC IG1105; K01383;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pREP10 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pcDV1/S2 from pcDV1 & linker
RC pRSVCAT/H2 from pRSVcat & linker
RC [pBT from BT gene]
RC pRSV/BT from pRSVCAT/H2 & pBT
RC pRSVPA1 from pRSV/BT & pcDV1/S2
RC pRSVPA2 from pRSVPA1 & linker
RC p220.2 from p201
RC p220.2/B- from p220.2
RC pREP2 from pRSVPA2 & p220.2/B-
RC pREP3 from pREP2
RC p220.2/XS from p220.2/B-
RC pHS1PA4 from pRSVPA2
RC pMEP4 from p220.2/XS & pHS1PA4
RC pRSVCAT-B2 from pRSVcat & linker
RC pRSVCAT-B2K from pRSVCAT-B2 & linker
RC pREP4 from pRSVCAT-B2K & pMEP4
RC pREP5 from pREP4 & oligo
RA Groger R.K., Morrow D.M., Tykocinski M.L.;
RT "Directional antisense and sense cDNA cloning using Epstein-Barr
RT virus episomal expression vectors";
RL Gene 81:285-294(1989).
XX
CC NM (pREP10)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Invitrogen)
CC HO (E.coli NM522)(E.coli INValphaF')(mammal)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (EBV)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pREP4 KpnI-BamHI 10128bp 462..10183..407
FT 2. oligo
FT BamHI-NaeI-StuI-XhoI-NotI-NheI-HindIII-PvuII-KpnI 60bp
FT \ gatcggtaccagctgaagcttgctagcggccgctcgaggccggcaaggcc
FT \ ggatccgtac
FT -> pREP5 10188bp
FT 1. pREP5 remove, 10188bp-
FT -> pREP10 8503bp"
FT misc_binding 1..1
FT /note="SIT XbaI"
FT polyA_signal 0..0
FT /note="PLA SV40 early gene"
FT misc_binding 0..0
FT /note="MCS
FT KpnI-PvuII-NheI-HindIII-NheI-NotI-XhoI-SfiI-BamHI"
FT promoter complement(0..0)
FT /note="PRO Rous sarcoma virus (RSV) long terminal
FT repeat (LTR)"
FT polyA_signal 0..0
FT /note="PLA SV40 thymidine kinase (TK) gene"
FT CDS complement(0..0)
FT /note="ANT E. coli hygromycin phosphotransferase gene;
FT hygromycin resistance gene (hyg)"
FT promoter complement(0..0)
FT /note="PRO SV40 thymidine kinase (TK) gene"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 4826..4826
FT /note="SIT XmnI"
FT misc_binding 5249..5249
FT /note="SIT ClaI"
FT misc_binding 5395..5395
FT /note="SIT StuI"
FT CDS 0..0
FT /note="GEN Epstein-Barr virus nuclear antigen gene
FT (EBNA-1)"
FT misc_binding 6411..6411
FT /note="SIT SacI"
FT rep_origin 0..0
FT /note="ORI Epstein-Barr virus (EBV) oriP"
FT misc_binding 8840..8840
FT /note="SIT EcoRV"
XX
SQ Sequence 8503 BP; 2069 A; 2212 C; 2260 G; 1962 T; 0 other;
tattgtatcg agctaggcac ttaaatacaa ttatctctgc aatgcggaat tcagtggttc
gtccaatcca tgtcagacct gtctgttgcc ttcctaataa ggcacgatcg taccacctta
cttccaccaa tcggcatgca cggtgctttt tctctccttg taaggcatgt tgctaactca
tcgttaccat gttgcaagac tacaagtgta ttgcataaga ctacatttcc ccctccctat
gcaaaagcga aactactata tcctgagggg actcctaacc gcgtacaacc gaagccccgc
tgggcgccta aacacaccct agtcccctca gatacgcgta tatctggccc gtacatcgcg
aagcagcgca aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg
ccttgttgta gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg
gagcagatac tggcttaact atgcggcagc agagcagatt gtactgagag tgcaccatac
aagctcagga tctgcgatga taagctgtca aacatgagaa ttggtcgacc actgggcgcc
agaaatccgc gcggtggttt ttgggggtcg ggggtgtttg gcagccacag acgcccggtg
ttcgtgtcgc gccagtacat gcggtccatg cccaggccat ccaaaaacca tgggtctgtc
tgctcagtcc agtcgtggac cagaccccac gcaacgccca aaataataac ccccacgaac
cataaaccat tccccatggg ggaccccgtc cctaacccac ggggccagtg gctatggcag
ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg
gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga
cagagtgcca gccctgggac cgaaccccgc gtttatgaac aaacgaccca acacccgtgc
gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc
cgtgtttcag ttagcctccc ccatctcccc tattcctttg ccctcggacg agtgctgggg
cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt
ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat
cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg
tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc
ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag
atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct
gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc
cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac
gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg
catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac
ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc
agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg
gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg
agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc
tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc attgcccggg atctgcggca
cgctgttgac gctgttaagc gggtcgctgc agggtcgctc ggtgttcgag gccacacgcg
tcaccttaat atgcgaagtg gacctgggac cgcgccgccc cgactgcatc tgcgtgttcg
aattcgccaa tgacaagacg ctgggcgggg tttgtgtcat catagaacta aagacatgca
aatatatttc ttccggggac accgccagca aacgcgagca acgggccacg gggatgaagc
agggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
ctcttaccag cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct
cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc
tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg
gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac
tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag
ccgcacgcgg cgcagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttacttcct ccgtgtggcc
gaacacgtcg agccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
ggccctttcg tcttcaagaa ttctcatgtt tgacagctta tcatcgataa gctgatcctc
acaggccgca cccagctttt cttccgttgc cccagtagca tctctgtctg gtgaccttga
agaggaagag gaggggtccc gagaatcccc atccctaccg tccagcaaaa agggggacga
ggaatttgag gcctggcttg aggctcagga cgcaaatctt gaggatgttc agcgggagtt
ttccgggctg cgagtaattg gtgatgagga cgaggatggt tcggaggatg gggaattttc
agacctggat ctgtctgaca gcgaccatga aggggatgag ggtggggggg ctgttggagg
gggcaggagt ctgcactccc tgtattcact gagcgtcgtc taataaagat gtctattgat
ctcttttagt gtgaatcatg tctgacgagg ggccaggtac aggacctgga aatggcctag
gagagaaggg agacacatct ggaccagaag gctccggcgg cagtggacct caaagaagag
ggggtgataa ccatggacga ggacggggaa gaggacgagg acgaggaggc ggaagaccag
gagccccggg cggctcagga tcagggccaa gacatagaga tggtgtccgg agaccccaaa
aacgtccaag ttgcattggc tgcaaaggga cccacggtgg aacaggagca ggagcaggag
cgggaggggc aggagcagga ggggcaggag ggaggccggg gtcgaggagg tagtggaggc
cggggtcgag gaggtagtgg aggccgccgg ggtagaggac gtgaaagagc caggggggga
agtcgtgaaa gagccagggg gagaggtcgt ggacgtggag aaaagaggcc caggagtccc
agtagtcagt catcatcatc cgggtctcca ccgcgcaggc cccctccagg tagaaggcca
tttttccacc ctgtagggga agccgattat tttgaatacc accaagaagg tggcccagat
ggtgagcctg acgtgccccc gggagcgata gagcagggcc ccgcagatga cccaggagaa
ggcccaagca ctggaccccg gggtcagggt gatggaggca ggcgcaaaaa aggagggtgg
tttggaaagc atcgtggtca aggaggttcc aacccgaaat ttgagaacat tgcagaaggt
ttaagagctc tcctggctag gagtcacgta gaaaggacta ccgacgaagg aacttgggtc
gccggtgtgt tcgtatatgg aggtagtaag acctcccttt acaacctaag gcgaggaact
gcccttgcta ttccacaatg tcgtcttaca ccattgagtc gtctcccctt tggaatggcc
cctggacccg gcccacaacc tggcccgcta agggagtcca ttgtctgtta tttcatggtc
tttttacaaa ctcatatatt tgctgaggtt ttgaaggatg cgattaagga ccttgttatg
acaaagcccg ctcctacctg caatatcagg gtgactgtgt gcagctttga cgatggagta
gatttgcctc cctggtttcc acctatggtg gaaggggctg ccgcggaggg tgatgacgga
gatgacggag atgaaggagg tgatggagat gagggtgagg aagggcagga gtgatgtaac
ttgttaggag acgccctcaa tcgtattaaa agccgtgtat tcccccgcac taaagaataa
atccccagta gacatcatgc gtgctgttgg tgtatttctg gccatctgtc ttgtcaccat
tttcgtcctc ccaacatggg gcaattgggc atacccatgt tgtcacgtca ctcagctccg
cgctcaacac cttctcgcgt tggaaaacat tagcgacatt tacctggtga gcaatcagac
atgcgacggc tttagcctgg cctccttaaa ttcacctaag aatgggagca accagcatgc
aggaaaagga caagcagcga aaattcacgc ccccttggga ggtggcggca tatgcaaagg
atagcactcc cactctacta ctgggtatca tatgctgact gtatatgcat gaggatagca
tatgctaccc ggatacagat taggatagca tatactaccc agatatagat taggatagca
tatgctaccc agatatagat taggatagcc tatgctaccc agatataaat taggatagca
tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
tatgctaccc agatatagat taggatagca tatgctaccc agatatagat taggatagca
tatgctatcc agatatttgg gtagtatatg ctacccagat ataaattagg atagcatata
ctaccctaat ctctattagg atagcatatg ctacccggat acagattagg atagcatata
ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
ctacccagat ataaattagg atagcatata ctacccagat atagattagg atagcatatg
ctacccagat atagattagg atagcctatg ctacccagat atagattagg atagcatatg
ctatccagat atttgggtag tatatgctac ccatggcaac attagcccac cgtgctctca
gcgacctcgt gaatatgagg accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg
taatttgtcc tccagatcgc agcaatcgcg cccctatctt ggcccgccca cctacttatg
caggtattcc ccggggtgcc attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt
agcggggtta caatcagcca agttattaca cccttatttt acagtccaaa accgcagggc
ggcgtgtggg ggctgacgcg tgcccccact ccacaatttc aaaaaaaaga gtggccactt
gtctttgttt atgggcccca ttggcgtgga gccccgttta attttcgggg gtgttagaga
caaccagtgg agtccgctgc tgtcggcgtc cactctcttt ccccttgtta caaatagagt
gtaacaacat ggttcacctg tcttggtccc tgcctgggac acatcttaat aaccccagta
tcatattgca ctaggattat gtgttgccca tagccataaa ttcgtgtgag atggacatcc
agtctttacg gcttgtcccc accccatgga tttctattgt taaagatatt cagaatgttt
cattcctaca ctagtattta ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc
acaatgccac cactgaaccc cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt
gttttcgagc acctcacata caccttactg ttcacaactc agcagttatt ctattagcta
aacgaaggag aatgaagaag caggcgaaga ttcaggagag ttcactgccc gctccttgat
cttcagccac tgcccttgtg actaaaatgg ttcactaccc tcgtggaatc ctgaccccat
gtaaataaaa ccgtgacagc tcatggggtg ggagatatcg ctgttcctta ggaccctttt
actaacccta attcgatagc atatgcttcc cgttgggtaa catatgctat tgaattaggg
ttagtctgga tagtatatac tactacccgg gaagcatatg ctacccgttt agggttaaca
agggggcctt ataaacacta ttgctaatgc cctcttgagg gtccgcttat cggtagctac
acaggcccct ctgattgacg ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg
tacatgtccc ccagcattgg tgtaagagct tcagccaaga gttacacata aaggcaatgt
tgtgttgcag tccacagact gcaaagtctg ctccaggatg aaagccactc agtgttggca
aatgtgcaca tccatttata aggatgtcaa ctacagtcag agaacccctt tgtgtttggt
ccccccccgt gtcacatgtg gaacagggcc cagttggcaa gttgtaccaa ccaactgaag
ggattacatg cactgccccg aatacaaaac aaaagcgctc ctcgtaccag cgaagaaggg
gcagagatgc cgtagtcagg tttagttcgt ccggcggcgg ggc
//