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ID PREP4 preliminary; circular DNA; SYN; 10183 BP.
XX
AC IG1106; K01383;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pREP4 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pcDV1/S2 from pcDV1 & linker
RC pRSVCAT/H2 from pRSVcat & linker
RC [pBT from BT gene]
RC pRSV/BT from pRSVCAT/H2 & pBT
RC pRSVPA1 from pRSV/BT & pcDV1/S2
RC pRSVPA2 from pRSVPA1 & linker
RC p220.2 from p201
RC p220.2/B- from p220.2
RC pREP2 from pRSVPA2 & p220.2/B-
RC pREP3 from pREP2
RC p220.2/XS from p220.2/B-
RC pHS1PA4 from pRSVPA2
RC pMEP4 from p220.2/XS & pHS1PA4
RC pRSVCAT-B2 from pRSVcat & linker
RC pRSVCAT-B2K from pRSVCAT-B2 & linker
RC pREP4 from pRSVCAT-B2K & pMEP4
RC pREP5 from pREP4 & oligo
RA Groger R.K., Morrow D.M., Tykocinski M.L.;
RT "Directional antisense and sense cDNA cloning using Epstein-Barr
RT virus episomal expression vectors";
RL Gene 81:285-294(1989).
XX
CC NM (pREP4)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Invitrogen)
CC HO (E.coli NM522)(E.coli INValphaF')(mammal)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (EBV)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pRSVcat NdeI 5028bp, 3' to RSV LTR
FT \ pSV2-cat 556..556
FT Klenow
FT BamHI linker 6bp ggatcc
FT -> pRSVCAT-B2 5028bp
FT 1. pRSVCAT-B2 HindIII 5028bp, RSV
FT Klenow
FT KpnI linker 6bp ggtacc
FT -> pRSVCAT-B2K 5028bp
FT 1. pMEP4 KpnI-BglII 9539bp 901..10440..45, ori/polyA
FT 2. pRSVCAT-B2K small BamHI-KpnI 600bp, RSV 3' LTR
FT \ pSV2-cat BamHI 3370
FT -> pREP4 10183bp"
FT misc_binding 1..1
FT /note="SIT XbaI"
FT polyA_signal 0..0
FT /note="PLA SV40 early gene"
FT misc_binding 0..0
FT /note="MCS
FT BamHI-SfiI-XhoI-NotI-NheI-HindIII-NheI-PvuII-KpnI"
FT promoter complement(0..0)
FT /note="PRO Rous sarcoma virus (RSV) long terminal
FT repeat (LTR)"
FT polyA_signal 0..0
FT /note="PLA SV40 thymidine kinase (TK) gene"
FT CDS complement(0..0)
FT /note="ANT E. coli hygromycin phosphotransferase gene;
FT hygromycin resistance gene (hyg)"
FT promoter complement(0..0)
FT /note="PRO SV40 thymidine kinase (TK) gene"
FT misc_binding 2747..2747
FT /note="SIT BstBI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 5255..5255
FT /note="SIT ClaI"
FT misc_binding 5401..5401
FT /note="SIT StuI"
FT CDS 0..0
FT /note="GEN Epstein-Barr virus nuclear antigen gene
FT (EBNA-1)"
FT misc_binding 7106..7106
FT /note="SIT SacI"
FT rep_origin 0..0
FT /note="ORI Epstein-Barr virus (EBV) oriP"
FT misc_binding 9535..9535
FT /note="SIT EcoRV"
XX
SQ Sequence 10183 BP; 2495 A; 2548 C; 2908 G; 2232 T; 0 other;
tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cggccttgcc
ggcctcgagc ggccgctagc aagcttgcta gcagctggta cccagcttgg aggtgcacac
caatgtggtg aatggtcaaa tggcgtttat tgtatcgagc taggcactta aatacaatta
tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacctgtc tgttgccttc
ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg tgctttttct
ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac aagtgtattg
cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc tgaggggact
cctaaccgcg tacaaccgaa gccccgctgg gcgcctaaac acaccctagt cccctcagat
acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt
cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc tcgcgcacta
ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg cggcagcaga
gcagattgta ctgagagtgc accatacaag ctcaggatct gcgatgataa gctgtcaaac
atgagaattg gtcgaccact gggcgccaga aatccgcgcg gtggtttttg ggggtcgggg
gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg gtccatgccc
aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggaccag accccacgca
acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga ccccgtccct
aacccacggg gccagtggct atggcagggc ctgccgcccc gacgttggct gcgagccctg
ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc
cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt
tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg
cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca tctcccctat
tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
atatctcatt gcccgggatc tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg
gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac ctgggaccgc
gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg ggcggggttt
gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc gccagcaaac
gcgagcaacg ggccacgggg atgaagcagg gcatggcggc cgacgcgctg ggctacgtct
tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
atccatcgcg tccgccatct ccagcagccg cacgcggcgc agcaaaaggc caggaaccgt
aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attctcatgt
ttgacagctt atcatcgata agctgatcct cacaggccgc acccagcttt tcttccgttg
ccccagtagc atctctgtct ggtgaccttg aagaggaaga ggaggggtcc cgagaatccc
catccctacc gtccagcaaa aagggggacg aggaatttga ggcctggctt gaggctcagg
acgcaaatct tgaggatgtt cagcgggagt tttccgggct gcgagtaatt ggtgatgagg
acgaggatgg ttcggaggat ggggaatttt cagacctgga tctgtctgac agcgaccatg
aaggggatga gggtgggggg gctgttggag ggggcaggag tctgcactcc ctgtattcac
tgagcgtcgt ctaataaaga tgtctattga tctcttttag tgtgaatcat gtctgacgag
gggccaggta caggacctgg aaatggccta ggagagaagg gagacacatc tggaccagaa
ggctccggcg gcagtggacc tcaaagaaga gggggtgata accatggacg aggacgggga
agaggacgag gacgaggagg cggaagacca ggagccccgg gcggctcagg atcagggcca
agacatagag atggtgtccg gagaccccaa aaacgtccaa gttgcattgg ctgcaaaggg
acccacggtg gaacaggagc aggagcagga gcgggagggg caggagcagg aggggcagga
gcaggaggag gggcaggagc aggaggaggg gcaggagggg caggaggggc aggaggggca
ggagcaggag gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca
ggaggagggg caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga
ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca
ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggagg ggcaggagca
ggaggggcag gaggggcagg aggggcagga gcaggagggg caggagcagg aggaggggca
ggaggggcag gaggggcagg agcaggaggg gcaggagcag gaggggcagg agcaggaggg
gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
ggggcaggag gggcaggagc aggaggaggg gcaggagggg caggagcagg aggaggggca
ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga
gcaggagggg caggaggggc aggagcagga ggaggggcag gagcaggagg ggcaggagca
ggaggtggag gccggggtcg aggaggcagt ggaggccggg gtcgaggagg tagtggaggc
cggggtcgag gaggtagtgg aggccgccgg ggtagaggac gtgaaagagc caggggggga
agtcgtgaaa gagccagggg gagaggtcgt ggacgtggag aaaagaggcc caggagtccc
agtagtcagt catcatcatc cgggtctcca ccgcgcaggc cccctccagg tagaaggcca
tttttccacc ctgtagggga agccgattat tttgaatacc accaagaagg tggcccagat
ggtgagcctg acgtgccccc gggagcgata gagcagggcc ccgcagatga cccaggagaa
ggcccaagca ctggaccccg gggtcagggt gatggaggca ggcgcaaaaa aggagggtgg
tttggaaagc atcgtggtca aggaggttcc aacccgaaat ttgagaacat tgcagaaggt
ttaagagctc tcctggctag gagtcacgta gaaaggacta ccgacgaagg aacttgggtc
gccggtgtgt tcgtatatgg aggtagtaag acctcccttt acaacctaag gcgaggaact
gcccttgcta ttccacaatg tcgtcttaca ccattgagtc gtctcccctt tggaatggcc
cctggacccg gcccacaacc tggcccgcta agggagtcca ttgtctgtta tttcatggtc
tttttacaaa ctcatatatt tgctgaggtt ttgaaggatg cgattaagga ccttgttatg
acaaagcccg ctcctacctg caatatcagg gtgactgtgt gcagctttga cgatggagta
gatttgcctc cctggtttcc acctatggtg gaaggggctg ccgcggaggg tgatgacgga
gatgacggag atgaaggagg tgatggagat gagggtgagg aagggcagga gtgatgtaac
ttgttaggag acgccctcaa tcgtattaaa agccgtgtat tcccccgcac taaagaataa
atccccagta gacatcatgc gtgctgttgg tgtatttctg gccatctgtc ttgtcaccat
tttcgtcctc ccaacatggg gcaattgggc atacccatgt tgtcacgtca ctcagctccg
cgctcaacac cttctcgcgt tggaaaacat tagcgacatt tacctggtga gcaatcagac
atgcgacggc tttagcctgg cctccttaaa ttcacctaag aatgggagca accagcatgc
aggaaaagga caagcagcga aaattcacgc ccccttggga ggtggcggca tatgcaaagg
atagcactcc cactctacta ctgggtatca tatgctgact gtatatgcat gaggatagca
tatgctaccc ggatacagat taggatagca tatactaccc agatatagat taggatagca
tatgctaccc agatatagat taggatagcc tatgctaccc agatataaat taggatagca
tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
tatgctaccc agatatagat taggatagca tatgctaccc agatatagat taggatagca
tatgctatcc agatatttgg gtagtatatg ctacccagat ataaattagg atagcatata
ctaccctaat ctctattagg atagcatatg ctacccggat acagattagg atagcatata
ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
ctacccagat ataaattagg atagcatata ctacccagat atagattagg atagcatatg
ctacccagat atagattagg atagcctatg ctacccagat atagattagg atagcatatg
ctatccagat atttgggtag tatatgctac ccatggcaac attagcccac cgtgctctca
gcgacctcgt gaatatgagg accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg
taatttgtcc tccagatcgc agcaatcgcg cccctatctt ggcccgccca cctacttatg
caggtattcc ccggggtgcc attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt
agcggggtta caatcagcca agttattaca cccttatttt acagtccaaa accgcagggc
ggcgtgtggg ggctgacgcg tgcccccact ccacaatttc aaaaaaaaga gtggccactt
gtctttgttt atgggcccca ttggcgtgga gccccgttta attttcgggg gtgttagaga
caaccagtgg agtccgctgc tgtcggcgtc cactctcttt ccccttgtta caaatagagt
gtaacaacat ggttcacctg tcttggtccc tgcctgggac acatcttaat aaccccagta
tcatattgca ctaggattat gtgttgccca tagccataaa ttcgtgtgag atggacatcc
agtctttacg gcttgtcccc accccatgga tttctattgt taaagatatt cagaatgttt
cattcctaca ctagtattta ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc
acaatgccac cactgaaccc cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt
gttttcgagc acctcacata caccttactg ttcacaactc agcagttatt ctattagcta
aacgaaggag aatgaagaag caggcgaaga ttcaggagag ttcactgccc gctccttgat
cttcagccac tgcccttgtg actaaaatgg ttcactaccc tcgtggaatc ctgaccccat
gtaaataaaa ccgtgacagc tcatggggtg ggagatatcg ctgttcctta ggaccctttt
actaacccta attcgatagc atatgcttcc cgttgggtaa catatgctat tgaattaggg
ttagtctgga tagtatatac tactacccgg gaagcatatg ctacccgttt agggttaaca
agggggcctt ataaacacta ttgctaatgc cctcttgagg gtccgcttat cggtagctac
acaggcccct ctgattgacg ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg
tacatgtccc ccagcattgg tgtaagagct tcagccaaga gttacacata aaggcaatgt
tgtgttgcag tccacagact gcaaagtctg ctccaggatg aaagccactc agtgttggca
aatgtgcaca tccatttata aggatgtcaa ctacagtcag agaacccctt tgtgtttggt
ccccccccgt gtcacatgtg gaacagggcc cagttggcaa gttgtaccaa ccaactgaag
ggattacatg cactgccccg aatacaaaac aaaagcgctc ctcgtaccag cgaagaaggg
gcagagatgc cgtagtcagg tttagttcgt ccggcggcgg ggc
//