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ID   PREP4      preliminary; circular DNA; SYN; 10183 BP.
XX
AC   IG1106; K01383;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pREP4 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pcDV1/S2 from pcDV1 & linker
RC   pRSVCAT/H2 from pRSVcat & linker
RC   [pBT from BT gene]
RC   pRSV/BT from pRSVCAT/H2 & pBT
RC   pRSVPA1 from pRSV/BT & pcDV1/S2
RC   pRSVPA2 from pRSVPA1 & linker
RC   p220.2 from p201
RC   p220.2/B- from p220.2
RC   pREP2 from pRSVPA2 & p220.2/B-
RC   pREP3 from pREP2
RC   p220.2/XS from p220.2/B-
RC   pHS1PA4 from pRSVPA2
RC   pMEP4 from p220.2/XS & pHS1PA4
RC   pRSVCAT-B2 from pRSVcat & linker
RC   pRSVCAT-B2K from pRSVCAT-B2 & linker
RC   pREP4 from pRSVCAT-B2K & pMEP4
RC   pREP5 from pREP4 & oligo
RA   Groger R.K., Morrow D.M., Tykocinski M.L.;
RT   "Directional antisense and sense cDNA cloning using Epstein-Barr
RT   virus episomal expression vectors";
RL   Gene 81:285-294(1989).
XX
CC   NM (pREP4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(mammal)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (EBV)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pRSVcat NdeI 5028bp, 3' to RSV LTR
FT                   \ pSV2-cat 556..556
FT                   Klenow
FT                   BamHI linker 6bp ggatcc
FT                   -> pRSVCAT-B2 5028bp
FT                   1. pRSVCAT-B2 HindIII 5028bp, RSV
FT                   Klenow
FT                   KpnI linker 6bp ggtacc
FT                   -> pRSVCAT-B2K 5028bp
FT                   1. pMEP4 KpnI-BglII 9539bp 901..10440..45, ori/polyA
FT                   2. pRSVCAT-B2K small BamHI-KpnI 600bp, RSV 3' LTR
FT                   \ pSV2-cat BamHI 3370
FT                   -> pREP4 10183bp"
FT   misc_binding    1..1
FT                   /note="SIT XbaI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    0..0
FT                   /note="MCS
FT                   BamHI-SfiI-XhoI-NotI-NheI-HindIII-NheI-PvuII-KpnI"
FT   promoter        complement(0..0)
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 thymidine kinase (TK) gene"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli hygromycin phosphotransferase gene;
FT                   hygromycin resistance gene (hyg)"
FT   promoter        complement(0..0)
FT                   /note="PRO SV40 thymidine kinase (TK) gene"
FT   misc_binding    2747..2747
FT                   /note="SIT BstBI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    5255..5255
FT                   /note="SIT ClaI"
FT   misc_binding    5401..5401
FT                   /note="SIT StuI"
FT   CDS             0..0
FT                   /note="GEN Epstein-Barr virus nuclear antigen gene
FT                   (EBNA-1)"
FT   misc_binding    7106..7106
FT                   /note="SIT SacI"
FT   rep_origin      0..0
FT                   /note="ORI Epstein-Barr virus (EBV) oriP"
FT   misc_binding    9535..9535
FT                   /note="SIT EcoRV"
XX
SQ   Sequence 10183 BP; 2495 A; 2548 C; 2908 G; 2232 T; 0 other;
     tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
     agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
     acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cggccttgcc
     ggcctcgagc ggccgctagc aagcttgcta gcagctggta cccagcttgg aggtgcacac
     caatgtggtg aatggtcaaa tggcgtttat tgtatcgagc taggcactta aatacaatta
     tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacctgtc tgttgccttc
     ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg tgctttttct
     ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac aagtgtattg
     cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc tgaggggact
     cctaaccgcg tacaaccgaa gccccgctgg gcgcctaaac acaccctagt cccctcagat
     acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt
     cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc tcgcgcacta
     ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg cggcagcaga
     gcagattgta ctgagagtgc accatacaag ctcaggatct gcgatgataa gctgtcaaac
     atgagaattg gtcgaccact gggcgccaga aatccgcgcg gtggtttttg ggggtcgggg
     gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg gtccatgccc
     aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggaccag accccacgca
     acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga ccccgtccct
     aacccacggg gccagtggct atggcagggc ctgccgcccc gacgttggct gcgagccctg
     ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc
     cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt
     tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg
     cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca tctcccctat
     tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
     cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
     gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
     ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
     cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
     caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
     atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
     gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
     agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
     tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
     attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
     gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
     tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
     ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
     cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
     acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
     ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
     atatctcatt gcccgggatc tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg
     gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac ctgggaccgc
     gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg ggcggggttt
     gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc gccagcaaac
     gcgagcaacg ggccacgggg atgaagcagg gcatggcggc cgacgcgctg ggctacgtct
     tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
     tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
     tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     atccatcgcg tccgccatct ccagcagccg cacgcggcgc agcaaaaggc caggaaccgt
     aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attctcatgt
     ttgacagctt atcatcgata agctgatcct cacaggccgc acccagcttt tcttccgttg
     ccccagtagc atctctgtct ggtgaccttg aagaggaaga ggaggggtcc cgagaatccc
     catccctacc gtccagcaaa aagggggacg aggaatttga ggcctggctt gaggctcagg
     acgcaaatct tgaggatgtt cagcgggagt tttccgggct gcgagtaatt ggtgatgagg
     acgaggatgg ttcggaggat ggggaatttt cagacctgga tctgtctgac agcgaccatg
     aaggggatga gggtgggggg gctgttggag ggggcaggag tctgcactcc ctgtattcac
     tgagcgtcgt ctaataaaga tgtctattga tctcttttag tgtgaatcat gtctgacgag
     gggccaggta caggacctgg aaatggccta ggagagaagg gagacacatc tggaccagaa
     ggctccggcg gcagtggacc tcaaagaaga gggggtgata accatggacg aggacgggga
     agaggacgag gacgaggagg cggaagacca ggagccccgg gcggctcagg atcagggcca
     agacatagag atggtgtccg gagaccccaa aaacgtccaa gttgcattgg ctgcaaaggg
     acccacggtg gaacaggagc aggagcagga gcgggagggg caggagcagg aggggcagga
     gcaggaggag gggcaggagc aggaggaggg gcaggagggg caggaggggc aggaggggca
     ggagcaggag gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca
     ggaggagggg caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga
     ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca
     ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggagg ggcaggagca
     ggaggggcag gaggggcagg aggggcagga gcaggagggg caggagcagg aggaggggca
     ggaggggcag gaggggcagg agcaggaggg gcaggagcag gaggggcagg agcaggaggg
     gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
     ggggcaggag gggcaggagc aggaggaggg gcaggagggg caggagcagg aggaggggca
     ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga
     gcaggagggg caggaggggc aggagcagga ggaggggcag gagcaggagg ggcaggagca
     ggaggtggag gccggggtcg aggaggcagt ggaggccggg gtcgaggagg tagtggaggc
     cggggtcgag gaggtagtgg aggccgccgg ggtagaggac gtgaaagagc caggggggga
     agtcgtgaaa gagccagggg gagaggtcgt ggacgtggag aaaagaggcc caggagtccc
     agtagtcagt catcatcatc cgggtctcca ccgcgcaggc cccctccagg tagaaggcca
     tttttccacc ctgtagggga agccgattat tttgaatacc accaagaagg tggcccagat
     ggtgagcctg acgtgccccc gggagcgata gagcagggcc ccgcagatga cccaggagaa
     ggcccaagca ctggaccccg gggtcagggt gatggaggca ggcgcaaaaa aggagggtgg
     tttggaaagc atcgtggtca aggaggttcc aacccgaaat ttgagaacat tgcagaaggt
     ttaagagctc tcctggctag gagtcacgta gaaaggacta ccgacgaagg aacttgggtc
     gccggtgtgt tcgtatatgg aggtagtaag acctcccttt acaacctaag gcgaggaact
     gcccttgcta ttccacaatg tcgtcttaca ccattgagtc gtctcccctt tggaatggcc
     cctggacccg gcccacaacc tggcccgcta agggagtcca ttgtctgtta tttcatggtc
     tttttacaaa ctcatatatt tgctgaggtt ttgaaggatg cgattaagga ccttgttatg
     acaaagcccg ctcctacctg caatatcagg gtgactgtgt gcagctttga cgatggagta
     gatttgcctc cctggtttcc acctatggtg gaaggggctg ccgcggaggg tgatgacgga
     gatgacggag atgaaggagg tgatggagat gagggtgagg aagggcagga gtgatgtaac
     ttgttaggag acgccctcaa tcgtattaaa agccgtgtat tcccccgcac taaagaataa
     atccccagta gacatcatgc gtgctgttgg tgtatttctg gccatctgtc ttgtcaccat
     tttcgtcctc ccaacatggg gcaattgggc atacccatgt tgtcacgtca ctcagctccg
     cgctcaacac cttctcgcgt tggaaaacat tagcgacatt tacctggtga gcaatcagac
     atgcgacggc tttagcctgg cctccttaaa ttcacctaag aatgggagca accagcatgc
     aggaaaagga caagcagcga aaattcacgc ccccttggga ggtggcggca tatgcaaagg
     atagcactcc cactctacta ctgggtatca tatgctgact gtatatgcat gaggatagca
     tatgctaccc ggatacagat taggatagca tatactaccc agatatagat taggatagca
     tatgctaccc agatatagat taggatagcc tatgctaccc agatataaat taggatagca
     tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
     tatgctaccc agatatagat taggatagca tatgctaccc agatatagat taggatagca
     tatgctatcc agatatttgg gtagtatatg ctacccagat ataaattagg atagcatata
     ctaccctaat ctctattagg atagcatatg ctacccggat acagattagg atagcatata
     ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
     ctacccagat ataaattagg atagcatata ctacccagat atagattagg atagcatatg
     ctacccagat atagattagg atagcctatg ctacccagat atagattagg atagcatatg
     ctatccagat atttgggtag tatatgctac ccatggcaac attagcccac cgtgctctca
     gcgacctcgt gaatatgagg accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg
     taatttgtcc tccagatcgc agcaatcgcg cccctatctt ggcccgccca cctacttatg
     caggtattcc ccggggtgcc attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt
     agcggggtta caatcagcca agttattaca cccttatttt acagtccaaa accgcagggc
     ggcgtgtggg ggctgacgcg tgcccccact ccacaatttc aaaaaaaaga gtggccactt
     gtctttgttt atgggcccca ttggcgtgga gccccgttta attttcgggg gtgttagaga
     caaccagtgg agtccgctgc tgtcggcgtc cactctcttt ccccttgtta caaatagagt
     gtaacaacat ggttcacctg tcttggtccc tgcctgggac acatcttaat aaccccagta
     tcatattgca ctaggattat gtgttgccca tagccataaa ttcgtgtgag atggacatcc
     agtctttacg gcttgtcccc accccatgga tttctattgt taaagatatt cagaatgttt
     cattcctaca ctagtattta ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc
     acaatgccac cactgaaccc cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt
     gttttcgagc acctcacata caccttactg ttcacaactc agcagttatt ctattagcta
     aacgaaggag aatgaagaag caggcgaaga ttcaggagag ttcactgccc gctccttgat
     cttcagccac tgcccttgtg actaaaatgg ttcactaccc tcgtggaatc ctgaccccat
     gtaaataaaa ccgtgacagc tcatggggtg ggagatatcg ctgttcctta ggaccctttt
     actaacccta attcgatagc atatgcttcc cgttgggtaa catatgctat tgaattaggg
     ttagtctgga tagtatatac tactacccgg gaagcatatg ctacccgttt agggttaaca
     agggggcctt ataaacacta ttgctaatgc cctcttgagg gtccgcttat cggtagctac
     acaggcccct ctgattgacg ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg
     tacatgtccc ccagcattgg tgtaagagct tcagccaaga gttacacata aaggcaatgt
     tgtgttgcag tccacagact gcaaagtctg ctccaggatg aaagccactc agtgttggca
     aatgtgcaca tccatttata aggatgtcaa ctacagtcag agaacccctt tgtgtttggt
     ccccccccgt gtcacatgtg gaacagggcc cagttggcaa gttgtaccaa ccaactgaag
     ggattacatg cactgccccg aatacaaaac aaaagcgctc ctcgtaccag cgaagaaggg
     gcagagatgc cgtagtcagg tttagttcgt ccggcggcgg ggc
//