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ID PREP7 preliminary; circular DNA; SYN; 9494 BP.
XX
AC IG1107; K01383;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pREP7 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pcDV1/S2 from pcDV1 & linker
RC pRSVCAT/H2 from pRSVcat & linker
RC [pBT from BT gene]
RC pRSV/BT from pRSVCAT/H2 & pBT
RC pRSVPA1 from pRSV/BT & pcDV1/S2
RC pRSVPA2 from pRSVPA1 & linker
RC p220.2 from p201
RC p220.2/B- from p220.2
RC pREP2 from pRSVPA2 & p220.2/B-
RC pREP3 from pREP2
RC p220.2/XS from p220.2/B-
RC pHS1PA4 from pRSVPA2
RC pMEP4 from p220.2/XS & pHS1PA4
RC pRSVCAT-B2 from pRSVcat & linker
RC pRSVCAT-B2K from pRSVCAT-B2 & linker
RC pREP4 from pRSVCAT-B2K & pMEP4
RC pREP5 from pREP4 & oligo
RA Groger R.K., Morrow D.M., Tykocinski M.L.;
RT "Directional antisense and sense cDNA cloning using Epstein-Barr
RT virus episomal expression vectors";
RL Gene 81:285-294(1989).
XX
CC NM (pREP7)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Invitrogen)
CC HO (E.coli NM522)(E.coli INValphaF')(mammal)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (EBV)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pREP4 KpnI-BamHI 10128bp 462..10183..407
FT 2. oligo
FT BamHI-NaeI-StuI-XhoI-NotI-NheI-HindIII-PvuII-KpnI 60bp
FT \ gatcggtaccagctgaagcttgctagcggccgctcgaggccggcaaggcc
FT \ ggatccgtac
FT -> pREP5 10188bp
FT 1. pREP5 remove, 10188bp-
FT -> pREP7 9494bp"
FT misc_binding 1..1
FT /note="SIT XbaI"
FT promoter 0..0
FT /note="PRO Rous sarcoma virus (RSV) long terminal
FT repeat (LTR)"
FT misc_binding 0..0
FT /note="MCS
FT KpnI-PvuII-NheI-HindIII-NheI-NotI-XhoI-SfiI-BamHI"
FT polyA_signal 0..0
FT /note="PLA SV40 early gene"
FT polyA_signal 0..0
FT /note="PLA SV40 thymidine kinase (TK) gene"
FT CDS complement(0..0)
FT /note="ANT E. coli hygromycin phosphotransferase gene;
FT hygromycin resistance gene (hyg)"
FT promoter complement(0..0)
FT /note="PRO SV40 thymidine kinase (TK) gene"
FT misc_binding 2749..2749
FT /note="SIT BstBI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 5259..5259
FT /note="SIT ClaI"
FT misc_binding 5405..5405
FT /note="SIT StuI"
FT CDS 0..0
FT /note="GEN Epstein-Barr virus nuclear antigen gene
FT (EBNA-1)"
FT misc_binding 6421..6421
FT /note="SIT SacI"
FT rep_origin 0..0
FT /note="ORI Epstein-Barr virus (EBV) oriP"
FT misc_binding 8850..8850
FT /note="SIT EcoRV"
XX
SQ Sequence 9494 BP; 2318 A; 2394 C; 2551 G; 2231 T; 0 other;
tctagagtcg accaattctc atgtttgaca gcttatcatc gcagatcctg agcttgtatg
gtgcactctc agtacaatct gctctgctgc cgcatagtta agccagtatc tgctccctgc
ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
tgtacgggcc agatatacgc gtatctgagg ggactagggt gtgtttaggc gcccagcggg
gcttcggttg tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg
gagggggaaa tgtagtctta tgcaatacac ttgtagtctt gcaacatggt aacgatgagt
tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
tggtacgatc gtgccttatt aggaaggcaa cagacaggtc tgacatggat tggacgaacc
actgaattcc gcattgcaga gataattgta tttaagtgcc tagctcgata caataaacgc
catttgacca ttcaccacat tggtgtgcac ctccaagctg ggtaccagct gctagcaagc
ttgctagcgg ccgctcgagg ccggcaaggc cggatccaga catgataaga tacattgatg
agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
gcattcattt tatgtttcag gttcaggggg aggtggggag gttttttaaa gcaagtaaaa
cctctacaaa tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac
ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
gccatgaccg gtcgaccact gggcgccaga aatccgcgcg gtggtttttg ggggtcgggg
gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg gtccatgccc
aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggaccag accccacgca
acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga ccccgtccct
aacccacggg gccagtggct atggcagggc ctgccgcccc gacgttggct gcgagccctg
ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc
cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt
tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg
cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca tctcccctat
tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
atatctcatt gcccgggatc tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg
gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac ctgggaccgc
gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg ggcggggttt
gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc gccagcaaac
gcgagcaacg ggccacgggg atgaagcagg gcatggcggc cgacgcgctg ggctacgtct
tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
atccatcgcg tccgccatct ccagcagccg cacgcggcgc agcaaaaggc caggaaccgt
aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attctcatgt
ttgacagctt atcatcgata agctgatcct cacaggccgc acccagcttt tcttccgttg
ccccagtagc atctctgtct ggtgaccttg aagaggaaga ggaggggtcc cgagaatccc
catccctacc gtccagcaaa aagggggacg aggaatttga ggcctggctt gaggctcagg
acgcaaatct tgaggatgtt cagcgggagt tttccgggct gcgagtaatt ggtgatgagg
acgaggatgg ttcggaggat ggggaatttt cagacctgga tctgtctgac agcgaccatg
aaggggatga gggtgggggg gctgttggag ggggcaggag tctgcactcc ctgtattcac
tgagcgtcgt ctaataaaga tgtctattga tctcttttag tgtgaatcat gtctgacgag
gggccaggta caggacctgg aaatggccta ggagagaagg gagacacatc tggaccagaa
ggctccggcg gcagtggacc tcaaagaaga gggggtgata accatggacg aggacgggga
agaggacgag gacgaggagg cggaagacca ggagccccgg gcggctcagg atcagggcca
agacatagag atggtgtccg gagaccccaa aaacgtccaa gttgcattgg ctgcaaaggg
acccacggtg gaacaggagc aggagcagga gcgggagggg caggagcagg aggggcagga
gggaggccgg ggtcgaggag gtagtggagg ccggggtcga ggaggtagtg gaggccgccg
gggtagagga cgtgaaagag ccaggggggg aagtcgtgaa agagccaggg ggagaggtcg
tggacgtgga gaaaagaggc ccaggagtcc cagtagtcag tcatcatcat ccgggtctcc
accgcgcagg ccccctccag gtagaaggcc atttttccac cctgtagggg aagccgatta
ttttgaatac caccaagaag gtggcccaga tggtgagcct gacgtgcccc cgggagcgat
agagcagggc cccgcagatg acccaggaga aggcccaagc actggacccc ggggtcaggg
tgatggaggc aggcgcaaaa aaggagggtg gtttggaaag catcgtggtc aaggaggttc
caacccgaaa tttgagaaca ttgcagaagg tttaagagct ctcctggcta ggagtcacgt
agaaaggact accgacgaag gaacttgggt cgccggtgtg ttcgtatatg gaggtagtaa
gacctccctt tacaacctaa ggcgaggaac tgcccttgct attccacaat gtcgtcttac
accattgagt cgtctcccct ttggaatggc ccctggaccc ggcccacaac ctggcccgct
aagggagtcc attgtctgtt atttcatggt ctttttacaa actcatatat ttgctgaggt
tttgaaggat gcgattaagg accttgttat gacaaagccc gctcctacct gcaatatcag
ggtgactgtg tgcagctttg acgatggagt agatttgcct ccctggtttc cacctatggt
ggaaggggct gccgcggagg gtgatgacgg agatgacgga gatgaaggag gtgatggaga
tgagggtgag gaagggcagg agtgatgtaa cttgttagga gacgccctca atcgtattaa
aagccgtgta ttcccccgca ctaaagaata aatccccagt agacatcatg cgtgctgttg
gtgtatttct ggccatctgt cttgtcacca ttttcgtcct cccaacatgg ggcaattggg
catacccatg ttgtcacgtc actcagctcc gcgctcaaca ccttctcgcg ttggaaaaca
ttagcgacat ttacctggtg agcaatcaga catgcgacgg ctttagcctg gcctccttaa
attcacctaa gaatgggagc aaccagcatg caggaaaagg acaagcagcg aaaattcacg
cccccttggg aggtggcggc atatgcaaag gatagcactc ccactctact actgggtatc
atatgctgac tgtatatgca tgaggatagc atatgctacc cggatacaga ttaggatagc
atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc
atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc
atatgctacc cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat
gctacccaga tataaattag gatagcatat actaccctaa tctctattag gatagcatat
gctacccgga tacagattag gatagcatat actacccaga tatagattag gatagcatat
gctacccaga tatagattag gatagcctat gctacccaga tataaattag gatagcatat
actacccaga tatagattag gatagcatat gctacccaga tatagattag gatagcctat
gctacccaga tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta
cccatggcaa cattagccca ccgtgctctc agcgacctcg tgaatatgag gaccaacaac
cctgtgcttg gcgctcaggc gcaagtgtgt gtaatttgtc ctccagatcg cagcaatcgc
gcccctatct tggcccgccc acctacttat gcaggtattc cccggggtgc cattagtggt
tttgtgggca agtggtttga ccgcagtggt tagcggggtt acaatcagcc aagttattac
acccttattt tacagtccaa aaccgcaggg cggcgtgtgg gggctgacgc gtgcccccac
tccacaattt caaaaaaaag agtggccact tgtctttgtt tatgggcccc attggcgtgg
agccccgttt aattttcggg ggtgttagag acaaccagtg gagtccgctg ctgtcggcgt
ccactctctt tccccttgtt acaaatagag tgtaacaaca tggttcacct gtcttggtcc
ctgcctggga cacatcttaa taaccccagt atcatattgc actaggatta tgtgttgccc
atagccataa attcgtgtga gatggacatc cagtctttac ggcttgtccc caccccatgg
atttctattg ttaaagatat tcagaatgtt tcattcctac actagtattt attgcccaag
gggtttgtga gggttatatt ggtgtcatag cacaatgcca ccactgaacc ccccgtccaa
attttattct gggggcgtca cctgaaacct tgttttcgag cacctcacat acaccttact
gttcacaact cagcagttat tctattagct aaacgaagga gaatgaagaa gcaggcgaag
attcaggaga gttcactgcc cgctccttga tcttcagcca ctgcccttgt gactaaaatg
gttcactacc ctcgtggaat cctgacccca tgtaaataaa accgtgacag ctcatggggt
gggagatatc gctgttcctt aggacccttt tactaaccct aattcgatag catatgcttc
ccgttgggta acatatgcta ttgaattagg gttagtctgg atagtatata ctactacccg
ggaagcatat gctacccgtt tagggttaac aagggggcct tataaacact attgctaatg
ccctcttgag ggtccgctta tcggtagcta cacaggcccc tctgattgac gttggtgtag
cctcccgtag tcttcctggg cccctgggag gtacatgtcc cccagcattg gtgtaagagc
ttcagccaag agttacacat aaaggcaatg ttgtgttgca gtccacagac tgcaaagtct
gctccaggat gaaagccact cagtgttggc aaatgtgcac atccatttat aaggatgtca
actacagtca gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc
ccagttggca agttgtacca accaactgaa gggattacat gcactgcccc gaatacaaaa
caaaagcgct cctcgtacca gcgaagaagg ggcagagatg ccgtagtcag gtttagttcg
tccggcggcg gggc
//