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ID PREP8 preliminary; circular DNA; SYN; 11811 BP.
XX
AC IG1108; K01383;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pREP8 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pcDV1/S2 from pcDV1 & linker
RC pRSVCAT/H2 from pRSVcat & linker
RC [pBT from BT gene]
RC pRSV/BT from pRSVCAT/H2 & pBT
RC pRSVPA1 from pRSV/BT & pcDV1/S2
RC pRSVPA2 from pRSVPA1 & linker
RC p220.2 from p201
RC p220.2/B- from p220.2
RC pREP2 from pRSVPA2 & p220.2/B-
RC pREP3 from pREP2
RC p220.2/XS from p220.2/B-
RC pHS1PA4 from pRSVPA2
RC pMEP4 from p220.2/XS & pHS1PA4
RC pRSVCAT-B2 from pRSVcat & linker
RC pRSVCAT-B2K from pRSVCAT-B2 & linker
RC pREP4 from pRSVCAT-B2K & pMEP4
RC pREP5 from pREP4 & oligo
RA Groger R.K., Morrow D.M., Tykocinski M.L.;
RT "Directional antisense and sense cDNA cloning using Epstein-Barr
RT virus episomal expression vectors";
RL Gene 81:285-294(1989).
XX
CC NM (pREP8)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Invitrogen)
CC HO (E.coli NM522)(E.coli INValphaF')(mammal)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (EBV)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pREP4 KpnI-BamHI 10128bp 462..10183..407
FT 2. oligo
FT BamHI-NaeI-StuI-XhoI-NotI-NheI-HindIII-PvuII-KpnI 60bp
FT \ gatcggtaccagctgaagcttgctagcggccgctcgaggccggcaaggcc
FT \ ggatccgtac
FT -> pREP5 10188bp
FT 1. pREP5 10188bp
FT -> pREP8 11811bp"
FT misc_binding 1..1
FT /note="SIT XbaI"
FT polyA_signal 0..0
FT /note="PLA SV40 early gene"
FT misc_binding 0..0
FT /note="MCS
FT BamHI-XhoI-NotI-NheI-HindIII-NheI-KpnI"
FT promoter complement(0..0)
FT /note="PRO Rous sarcoma virus (RSV) long terminal
FT repeat (LTR)"
FT misc_binding 859..859
FT /note="SIT NruI"
FT polyA_signal 0..0
FT /note="PLA SV40 thymidine kinase (TK) gene"
FT CDS complement(0..0)
FT /note="ANT E. coli histidinol dehydrogenase gene
FT histidinol resistance gene (his)"
FT promoter complement(0..0)
FT /note="PRO SV40"
FT misc_binding 2747..2747
FT /note="SIT TthIIII"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 6825..6825
FT /note="SIT BstEII"
FT CDS 0..0
FT /note="GEN Epstein-Barr virus nuclear antigen gene
FT (EBNA-1)"
FT misc_binding 8609..8609
FT /note="SIT SacI"
FT rep_origin 0..0
FT /note="ORI Epstein-Barr virus (EBV) oriP"
FT misc_binding 11037..11037
FT /note="SIT EcoRV"
XX
SQ Sequence 11811 BP; 3010 A; 2789 C; 3272 G; 2740 T; 0 other;
tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cggccttgcc
ggcctcgagc ggccgctagc aagcttgcta gcagctggta cccagcttgg aggtgcacac
caatgtggtg aatggtcaaa tggcgtttat tgtatcgagc taggcactta aatacaatat
ctctgcaatg cggaattcag tggttcgtcc aatccatgtc agacccgtct gttgccttcc
taataaggca cgatcgtacc accttacttc caccaatcgg catgcacggt gctttttctc
tccttgtaag gcatgttgct aactcatcgt taccatgttg caagactaca agagtattgc
ataagactac atttccccct ccctatgcaa aagcgaaact actatatcct gaggggactc
ctaaccgcgt acaaccgaag ccccgctttt cgcctaaaca caccctagtc ccctcagata
cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc
ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac
tcagcgacct ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag
cagattgtac tgagagtgca ccatacggat ctgcgatgat aagctgtcaa acatgagaat
tggtcgacct gcagccagct cagatcttga attcctttgc ctaatttaaa tgaggactta
acctgtggaa atattttgat gtgggaagct gttactgtta aaactgaggt tattggggta
actgctatgt taaacttgca ttcagggaca caaaaaactc atgaaaatgg tgctggaaaa
cccattcaag ggtcaaattt tcattttttt gctgttggtg gggaaccttt ggagctgcag
ggtgtgttag caaactacag gaccaaatat cctgctcaaa ctgtaacccc aaaaaatgct
acagttgaca gtcagcagat gaacactgac cacaaggctg ttttggataa ggaaatgctt
atccagtgga gtgctgggtt cctgatccaa gtaaaaatga aaacactaga tattttggaa
cctacacagg tggggaaaat gtgcctcctg ttttgcacat tactaacaca gcaaccacag
tgcttcttga tgagcagggt gttgggccct tgtgcaaagc tgacagcttg tatgtttctg
ctgttgacat ttgtgggctg tttaccaaca cttctggaac acagcagtgg aagggacttc
ccagatattt taaaattacc cttagaaagc ggtctgtgaa aaacccctac ccaatttcct
ttttgttaag tgacctaatt aacaggagga cacagagggt ggatgggcag cctatgattg
gaatgtcctc tcaagtagag gaggttaggg tttatgagga cacagaggag cttcctgggg
atcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct
ctagtcaagg cactatacat caaatattcc ttattaaccc ctttacaaat taaaaagcta
aaggtacaca atttttgagc atagttatta atagcagaca ctctatgcct gtgtggagta
agaaaaaaca gtatgttatg attataactg ttatgcctac ttataaaggt tacagaatat
ttttccataa ttttcttgta tagcagtgca gctttttcct ttgtggtgta aatagcaaag
caagcaagag ttctattact aaacacagca tgactcaaaa aacttagcaa ttctgaagga
aagtccttgg ggtcttctac ctttctcttc ttttttggag gagtagaatg ttgagagtca
gcagtagcct catcatcact agatggcatt tcttctgagc aaaacaggtt ttcctcatta
aaggcattcc accactgctc ccattcatca gttccatagg ttggaatcta aaatacacaa
acaattagaa tcagtagttt aacacattat acacttaaaa attttatatt taccttagag
ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta aggttccttc
acaaagatcc cgggctaagt cagcgacgct gagagtgttt tcagtgctca tgcttgctcc
ttgagggcgt ttacgcgcag ggtcacggca tttttatggg cggtcagacg ttctgccgcc
gccaatgttt caatggttga tgccagagcg gaaaagcccg ctttcgacag ttcctgaacg
gtcatccgtt tctggaaatc cgctaaccca aggctggaac aggtagcagt atagccatag
gtcggtaaaa catggttggt tccggaagcg taatcaccgg cggattccgg cgaccagtcg
ccgagaaata ccgagcctgc gctggtaatc gcatccacca aatcgcgcgc attgcgcgtc
tggatgatta agtgttccgg cccatactga ttagagatgg cgacgcactg cgctaaatct
ttggtcacaa tcagacgact ggcgctcagg gcctgccggg cggtgtccgc gcgcggcagt
tccgccagtt gacgttctac cgcctccgcc accttgcggg caatgtcagc atcaggcgtc
agcaggatca cctgggaatc cgggccgtgc tcagcctggg agagcaggtc agaagcgacg
aaatccggtg ttgcgccgct gtctgcgatc accagtactt cagacggccc ggctggcata
tcgatagccg cgccgtcgag acgctggctg acctgacgtt tggcttcggt tacaaaggcg
ttgccggggc caaaaatttt atccactttc ggtacggact cgctgccgaa ggccagagcg
gcaatcgcct gcgcgccgcc gacgttaaag atttcctgca cgccacacag ttgcgccgca
tagaggattt catcagcgat gggcggcggc gagcacagaa ccaccttctg gcatcccgca
atgcgcgccg gcgtcgccag catcagcacc gttgagaaga gcggagccga gccgccggga
atatacagac cgacagacga gacgggacgc gtaacctgct ggcaacgcac gcctggctgg
gtttccacat ctacaggcgg tagcgtctgc gcggaatgga acgtttcaat atttttgacg
gcagcggtca tcgcctgttt taattcgtcg ctcagacgcg cgccggcggc ggcgatctct
tcaggggtga cgcgtagcgc tgtcacttct gttttatcaa atttagcgct gtattcacgc
agggcatcgt caccgcgcgt ttttacatta tccagaatat cgctgaccgt ccgggtaata
ctgtcagagg cggaaatcgc cggacgcgtc agcagcgcac gctgctgttc agggctacag
ctgttccagt caatcagggt attgaagctc atggtccggg atctgagctt tttgcaaaag
cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
tcggcctctg cataaaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggttgctgac taattgagat
gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
acacattcca cagctgcctc gcgcgtttcg gtgatgacgg tgaaaaacct ctgacacatg
cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
gatagcggag tgtatcagat ctgcgacgcg aggctggatg gccttcccca ttatgattct
tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga
tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat
cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt
ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc
atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc
actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct
tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg cagcaaaagg
ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
aattctcatg tttgacagct tatcatcgat aagctgatcc tcacaggccg cacccagctt
ttcttccgtt gccccagtag catctctgtc tggtgacctt gaagaggaag aggaggggtc
ccgagaatcc ccatccctac cgtccagcaa aaagggggac gaggaatttg aggcctggct
tgaggctcag gacgcaaatc ttgaggatgt tcagcgggag ttttccgggc tgcgagtaat
tggtgatgag gacgaggatg gttcggagga tggggaattt tcagacctgg atctgtctga
cagcgaccat gaaggggatg agggtggggg ggctgttgga gggggcagga gtctgcactc
cctgtattca ctgagcgtcg tctaataaag atgtctattg atctctttta gtgtgaatca
tgtctgacga ggggccaggt acaggacctg gaaatggcct aggagagaag ggagacacat
ctggaccaga aggctccggc ggcagtggac ctcaaagaag agggggtgat aaccatggac
gaggacgggg aagaggacga ggacgaggag gcggaagacc aggagccccg ggcggctcag
gatcagggcc aagacataga gatggtgtcc ggagacccca aaaacgtcca agttgcattg
gctgcaaagg gacccacggt ggaacaggag caggagcagg agcgggaggg gcaggagcag
gaggggcagg agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg
caggaggggc aggagcagga ggaggggcag gagcaggagg aggggcagga ggggcaggag
gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca ggagcaggag
gaggggcagg aggggcagga ggggcaggag caggaggagg ggcaggagca ggaggagggg
caggaggggc aggagcagga ggaggggcag gaggggcagg aggggcagga gcaggaggag
gggcaggagc aggaggggca ggaggggcag gaggggcagg agcaggaggg gcaggagcag
gaggaggggc aggaggggca ggaggggcag gagcaggagg ggcaggagca ggaggggcag
gagcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggaggg gcaggagggg
caggagcagg aggggcagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag
gaggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag
gaggggcagg agcaggaggg gcaggagggg caggagcagg aggaggggca ggagcaggag
gggcaggagc aggaggtgga ggccggggtc gaggaggcag tggaggccgg ggtcgaggag
gtagtggagg ccggggtcga ggaggtagtg gaggccgccg gggtagagga cgtgaaagag
ccaggggggg aagtcgtgaa agagccaggg ggagaggtcg tggacgtgga gaaaagaggc
ccaggagtcc cagtagtcag tcatcatcat ccgggtctcc accgcgcagg ccccctccag
gtagaaggcc atttttccac cctgtagggg aagccgatta ttttgaatac caccaagaag
gtggcccaga tggtgagcct gacgtgcccc cgggagcgat agagcagggc cccgcagatg
acccaggaga aggcccaagc actggacccc ggggtcaggg tgatggaggc aggcgcaaaa
aaggagggtg gtttggaaag catcgtggtc aaggaggttc caacccgaaa tttgagaaca
ttgcagaagg tttaagagct ctcctggcta ggagtcacgt agaaaggact accgacgaag
gaacttgggt cgccggtgtg ttcgtatatg gaggtagtaa gacctccctt tacaacctaa
ggcgaggaac tgcccttgct attccacaat gtcgtcttac accattgagt cgtctcccct
ttggaatggc ccctggaccc ggcccacaac ctggcccgct aagggagtcc attgtctgtt
atttcatggt ctttttacaa actcatatat ttgctgaggt tttgaaggat gcgattaagg
accttgttat gacaaagccc gctcctacct gcaatatcag ggtgactgtg tgcagctttg
acgatggagt agatttgcct ccctggtttc cacctatggt ggaaggggct gccgcggagg
gtgatgacgg agatgacgga gatgaaggag gtgatggaga tgagggtgag gaagggcagg
agtgatgtaa cttgttagga gacgccctca atcgtattaa aagccgtgta ttcccccgca
ctaaagaata aatccccagt agacatcatg cgtgctgttg gtgtatttct ggccatctgt
cttgtcacca ttttcgtcct cccaacatgg ggcaattggg catacccatg ttgtcacgtc
actcagctcc gcgctcaaca ccttctcgcg ttggaaaaca ttagcgacat ttacctggtg
agcaatcaga catgcgacgg ctttagcctg gcctccttaa attcacctaa gaatgggagc
aaccagcatg caggaaaagg acaagcagcg aaaattcacg cccccttggg aggtggcggc
atatgcaaag gatagcactc ccactctact actgggtatc atatgctgac tgtatatgca
tgaggatagc atatgctacc cggatacaga ttaggatagc atatactacc cagatataga
ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc cagatataaa
ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc cagatataga
ttaggatagc ctatgctacc cagatataga ttaggatagc atatgctacc cagatataga
ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccaga tataaattag
gatagcatat actaccctaa tctctattag gatagcatat gctacccgga tacagattag
gatagcatat actacccaga tatagattag gatagcatat gctacccaga tatagattag
gatagcctat gctacccaga tataaattag gatagcatat actacccaga tatagattag
gatagcatat gctacccaga tatagattag gatagcctat gctacccaga tatagattag
gatagcatat gctatccaga tatttgggta gtatatgcta cccatggcaa cattagccca
ccgtgctctc agcgacctcg tgaatatgag gaccaacaac cctgtgcttg gcgctcaggc
gcaagtgtgt gtaatttgtc ctccagatcg cagcaatcgc gcccctatct tggcccgccc
acctacttat gcaggtattc cccggggtgc cattagtggt tttgtgggca agtggtttga
ccgcagtggt tagcggggtt acaatcagcc aagttattac acccttattt tacagtccaa
aaccgcaggg cggcgtgtgg gggctgacgc gtgcccccac tccacaattt caaaaaaaag
agtggccact tgtctttgtt tatgggcccc attggcgtgg agccccgttt aattttcggg
ggtgttagag acaaccagtg gagtccgctg ctgtcggcgt ccactctctt tccccttgtt
acaaatagag tgtaacaaca tggttcacct gtcttggtcc ctgcctggga cacatcttaa
taaccccagt atcatattgc actaggatta tgtgttgccc atagccataa attcgtgtga
gatggacatc cagtctttac ggcttgtccc caccccatgg atttctattg ttaaagatat
tcagaatgtt tcattcctac actagtattt attgcccaag gggtttgtga gggttatatt
ggtgtcatag cacaatgcca ccactgaacc ccccgtccaa attttattct gggggcgtca
cctgaaacct tgttttcgag cacctcacat acaccttact gttcacaact cagcagttat
tctattagct aaacgaagga gaatgaagaa gcaggcgaag attcaggaga gttcactgcc
cgctccttga tcttcagcca ctgcccttgt gactaaaatg gttcactacc ctcgtggaat
cctgacccca tgtaaataaa accgtgacag ctcatggggt gggagatatc gctgttcctt
aggacccttt tactaaccct aattcgatag catatgcttc ccgttgggta acatatgcta
ttgaattagg gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt
tagggttaac aagggggcct tataaacact attgctaatg ccctcttgag ggtccgctta
tcggtagcta cacaggcccc tctgattgac gttggtgtag cctcccgtag tcttcctggg
cccctgggag gtacatgtcc cccagcattg gtgtaagagc ttcagccaag agttacacat
aaaggcaatg ttgtgttgca gtccacagac tgcaaagtct gctccaggat gaaagccact
cagtgttggc aaatgtgcac atccatttat aaggatgtca actacagtca gagaacccct
ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc ccagttggca agttgtacca
accaactgaa gggattacat gcactgcccc gaatacaaaa caaaagcgct cctcgtacca
gcgaagaagg ggcagagatg ccgtagtcag gtttagttcg tccggcggcg g
//