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ID PRIT2T preliminary; circular DNA; SYN; 4229 BP.
XX
AC IG0198;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pRIT2T - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pRIT1 from pSPA11 & pCL19delta YT
RC pUN672 from pRIT1 & pEMBL9
RC pRIT2 from pUN672
RC pRIT3 from pSPA16 & pEMBL9
RC pRIT4 from pRIT3 & pC194
RC pRIT5 from pRIT4
RC pRIT6 from pRIT5 & pCH40
RC pRIT2T from pRIT2
RA Nilsson B., Abrahmsen L., Uhlen M.;
RT "Immobilization and purification of enzymes with staphylococcal
RT protein A gene fusion vectors";
RL EMBO J. 4:1075-1080(1985).
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RN [2]
RC pNF2690 from lambda, cI857 gene
RA Fiil N.;
RT ;
RL Unpublished (1985).
XX
RN [3]
RC pCH40 from pBR322 & phoA gene
RA Hofmann C., Wright A.;
RT ;
RL Unpublished (1985).
XX
CC Protein A has IgG-binding domains allowing purification of
CC fusion protein on IgG Sepharose 6FF.
CC NM (pRIT2T)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Pharmacia)
CC HO (E.coli N4830-1)
CC CP ()
CC FN (expression)
CC SE (affinity)
CC PA (pRIT2 from pEMBL9 and pSPA11 and pCL19deltaYT)
CC BR (pRIT3)(pRIT4)(pRIT5)(pRIT6)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pSPA11 BclI-BamHI 800bp
FT 2. pCL19delta YT BamHI
FT -> pRIT1 [cro/protein A/lacZ fusion]
FT 1. pRIT1 EcoRI-EcoRI
FT 2. pEMBL9+ EcoRI 3939bp 259..259
FT -> pUN672
FT 1. pUN672 ClaI-EcoRI
FT Klenow:Klenow
FT BglII linker 6bp agatct:BglII linker 6bp agatct
FT BglII-BglII 4300bp
FT -> pRIT2
FT 1. pRIT2
FT -> pRIT2T 4229bp"
FT misc_binding 0..0
FT /note="MCS unique EcoRI-SmaI-BamHI-SalI-PstI"
FT misc_binding 0..0
FT /note="SIT EcoRV"
FT terminator 0..0
FT /note="TER E. coli protein A gene
FT transcription and translation"
FT misc_binding 0..0
FT /note="SIT unique PvuII"
FT rep_origin complement(0..0)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 0..0
FT /note="SIT unique PvuI"
FT promoter 0..0
FT /note="PRO bacteriophage lambda cro gene rightward
FT p-r, BglII-HindIII fragment"
FT misc_feature 0..0
FT /note="ATG bacteriophage lambda cro gene"
FT misc_binding 0..0
FT /note="SIT unique BglII"
FT CDS 0..0
FT /note="GEN bacteriophage lambda protein A gene"
XX
SQ Sequence 4229 BP; 1313 A; 958 C; 886 G; 1072 T; 0 other;
ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
ttttctacgg ggtctcacgc tcagtggaac gaaaactcac gttagaggat tttggtcatg
agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
taccgcatca aattccaaca tccaataaat catacaggca aggcaaagaa ttagcaaaat
taagcaataa agcctcagag cataaagcta aatcggttgt accaaaaaca ttatgaccct
gtaatacttt tgcgggagaa gcctttattt caacgcaagg ataaaaattt ttagaaccct
catatatttt aaatgcaatg cctgagtaat gtgtaggtaa agattcaaac gggtgagaaa
ggccggagac agtcaaatca ccatcaatat gatattcaac cgttctagct gataaattca
tgccggagag ggtagctatt tttgagaggt ctacaaaggc tatcaggtca ttgcctgaga
gtctggagca aacaagagaa tcgcagatct gaattaattc cattccgcaa gggaataaat
atctaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt gcatgtacta
aggaggttgt atggaacaac gcataaccct gaaagaagct tgggatcaac gcaatggttt
tatccaaagc cttaaagatg atccaagcca aagtgctaac gttttaggtg aagctcaaaa
acttaatgac tctcaagctc caaaagctga tgcgcaacaa aataacttca acaaagatca
acaaagcgcc ttctatgaaa tcttgaacat gcctaactta aacgaagcgc aacgtaacgg
cttcattcaa agtcttaaag acgacccaag ccaaagcact aacgttttag gtgaagctaa
aaaattaaac gaatctcaag caccgaaagc tgataacaat ttcaacaaag aacaacaaaa
tgctttctat gaaatcttga atatgcctaa cttaaacgaa gaacaacgca atggtttcat
ccaaagctta aaagatgacc caagccaaag tgctaaccta ttgtcagaag ctaaaaagtt
aaatgaatct caagcaccga aagcggataa caaattcaac aaagaacaac aaaatgcttt
ctatgaaatc ttacatttac ctaacttaaa cgaagaacaa cgcaatggtt tcatccaaag
cctaaaagat gacccaagcc aaagcgctaa ccttttagca gaagctaaaa agctaaatga
tgctcaagca ccaaaagctg acaacaaatt caacaaagaa caacaaaatg ctttctatga
aattttacat ttacctaact taactgaaga acaacgtaac ggcttcatcc aaagccttaa
agacgatccg gggaattccc ggggatccgt cgacctgcag ataacaaatt agctgataaa
aacatgatca aacctggtca agaacttgtt gttgataaga agcaaccagc aaaccatgca
gatgctaaca aagctcaagc attaccagaa actggcgaag aaaatccatt catcggtaca
actgtatttg gtggattatc attagcctta ggtgcagcgt tattagctgg acgtcgtcgc
gaactataaa aacaaacaat acacaacgat agatatcatt ttatgcaaac caattttaac
ttatatacgt tgattaacac attcttattt gaaatgataa gaatcatcta aatgcacgag
caacatcttt tgttgctcag tgcatttttt attttactta cttttctaaa caacttctga
aacgcctcaa cactttctac tctgattaca tatatgacat ttttacggat taaaaaatcg
aactagacaa gatcgtcatt gcatttcgta ctagttcgat tcatgaataa ttagatttaa
aatgtcattt gaatccaagt gacaacatta tttatattta gaatattaac gttagtataa
acgtccaaac acaaataaaa gcaacaaata taatactgta ttttaacgtc atttttaata
atgcagattc ttcaccactt ttttaacag
//