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ID   PRIT2T     preliminary; circular DNA; SYN; 4229 BP.
XX
AC   IG0198;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pRIT2T - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRIT1 from pSPA11 & pCL19delta YT
RC   pUN672 from pRIT1 & pEMBL9
RC   pRIT2 from pUN672
RC   pRIT3 from pSPA16 & pEMBL9
RC   pRIT4 from pRIT3 & pC194
RC   pRIT5 from pRIT4
RC   pRIT6 from pRIT5 & pCH40
RC   pRIT2T from pRIT2
RA   Nilsson B., Abrahmsen L., Uhlen M.;
RT   "Immobilization and purification of enzymes with staphylococcal
RT   protein A gene fusion vectors";
RL   EMBO J. 4:1075-1080(1985).
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RN   [2]
RC   pNF2690 from lambda, cI857 gene
RA   Fiil N.;
RT   ;
RL   Unpublished (1985).
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RN   [3]
RC   pCH40 from pBR322 & phoA gene
RA   Hofmann C., Wright A.;
RT   ;
RL   Unpublished (1985).
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CC   Protein A has IgG-binding domains allowing purification of
CC   fusion protein on IgG Sepharose 6FF.
CC   NM (pRIT2T)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Pharmacia)
CC   HO (E.coli N4830-1)
CC   CP ()
CC   FN (expression)
CC   SE (affinity)
CC   PA (pRIT2 from pEMBL9 and pSPA11 and pCL19deltaYT)
CC   BR (pRIT3)(pRIT4)(pRIT5)(pRIT6)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSPA11 BclI-BamHI 800bp
FT                   2. pCL19delta YT BamHI
FT                   -> pRIT1 [cro/protein A/lacZ fusion]
FT                   1. pRIT1 EcoRI-EcoRI
FT                   2. pEMBL9+ EcoRI 3939bp 259..259
FT                   -> pUN672
FT                   1. pUN672 ClaI-EcoRI
FT                   Klenow:Klenow
FT                   BglII linker 6bp agatct:BglII linker 6bp agatct
FT                   BglII-BglII 4300bp
FT                   -> pRIT2
FT                   1. pRIT2
FT                   -> pRIT2T 4229bp"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-SmaI-BamHI-SalI-PstI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRV"
FT   terminator      0..0
FT                   /note="TER E. coli protein A gene
FT                   transcription and translation"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuII"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda cro gene rightward
FT                   p-r, BglII-HindIII fragment"
FT   misc_feature    0..0
FT                   /note="ATG bacteriophage lambda cro gene"
FT   misc_binding    0..0
FT                   /note="SIT unique BglII"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda protein A gene"
XX
SQ   Sequence 4229 BP; 1313 A; 958 C; 886 G; 1072 T; 0 other;
     ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     ttttctacgg ggtctcacgc tcagtggaac gaaaactcac gttagaggat tttggtcatg
     agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     taccgcatca aattccaaca tccaataaat catacaggca aggcaaagaa ttagcaaaat
     taagcaataa agcctcagag cataaagcta aatcggttgt accaaaaaca ttatgaccct
     gtaatacttt tgcgggagaa gcctttattt caacgcaagg ataaaaattt ttagaaccct
     catatatttt aaatgcaatg cctgagtaat gtgtaggtaa agattcaaac gggtgagaaa
     ggccggagac agtcaaatca ccatcaatat gatattcaac cgttctagct gataaattca
     tgccggagag ggtagctatt tttgagaggt ctacaaaggc tatcaggtca ttgcctgaga
     gtctggagca aacaagagaa tcgcagatct gaattaattc cattccgcaa gggaataaat
     atctaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt gcatgtacta
     aggaggttgt atggaacaac gcataaccct gaaagaagct tgggatcaac gcaatggttt
     tatccaaagc cttaaagatg atccaagcca aagtgctaac gttttaggtg aagctcaaaa
     acttaatgac tctcaagctc caaaagctga tgcgcaacaa aataacttca acaaagatca
     acaaagcgcc ttctatgaaa tcttgaacat gcctaactta aacgaagcgc aacgtaacgg
     cttcattcaa agtcttaaag acgacccaag ccaaagcact aacgttttag gtgaagctaa
     aaaattaaac gaatctcaag caccgaaagc tgataacaat ttcaacaaag aacaacaaaa
     tgctttctat gaaatcttga atatgcctaa cttaaacgaa gaacaacgca atggtttcat
     ccaaagctta aaagatgacc caagccaaag tgctaaccta ttgtcagaag ctaaaaagtt
     aaatgaatct caagcaccga aagcggataa caaattcaac aaagaacaac aaaatgcttt
     ctatgaaatc ttacatttac ctaacttaaa cgaagaacaa cgcaatggtt tcatccaaag
     cctaaaagat gacccaagcc aaagcgctaa ccttttagca gaagctaaaa agctaaatga
     tgctcaagca ccaaaagctg acaacaaatt caacaaagaa caacaaaatg ctttctatga
     aattttacat ttacctaact taactgaaga acaacgtaac ggcttcatcc aaagccttaa
     agacgatccg gggaattccc ggggatccgt cgacctgcag ataacaaatt agctgataaa
     aacatgatca aacctggtca agaacttgtt gttgataaga agcaaccagc aaaccatgca
     gatgctaaca aagctcaagc attaccagaa actggcgaag aaaatccatt catcggtaca
     actgtatttg gtggattatc attagcctta ggtgcagcgt tattagctgg acgtcgtcgc
     gaactataaa aacaaacaat acacaacgat agatatcatt ttatgcaaac caattttaac
     ttatatacgt tgattaacac attcttattt gaaatgataa gaatcatcta aatgcacgag
     caacatcttt tgttgctcag tgcatttttt attttactta cttttctaaa caacttctga
     aacgcctcaa cactttctac tctgattaca tatatgacat ttttacggat taaaaaatcg
     aactagacaa gatcgtcatt gcatttcgta ctagttcgat tcatgaataa ttagatttaa
     aatgtcattt gaatccaagt gacaacatta tttatattta gaatattaac gttagtataa
     acgtccaaac acaaataaaa gcaacaaata taatactgta ttttaacgtc atttttaata
     atgcagattc ttcaccactt ttttaacag
//