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ID   PRJF1      preliminary; circular DNA; PRO; 2631 BP.
XX
AC   M94552;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Butyrivibrio plasmid vector pRJF1 - complete.
XX
KW   cloning vector.
XX
OS   Butyrivibrio fibrisolvens
OC   Prokaryota.
XX
RN   [1]
RP   1-2631
RC   pRJF1 from Butyrivibrio
RC   pBE2.1, pBE2.2, pBE2.6, pBE2.7 from pRJF1 & pUC118
RC   pBH3.3, pBH3.7 from pRJF1 & pUC118
RA   Hefford M.A., Teather R.M., Forster R.J.;
RT   "The complete nucleotide sequence of a small, cryptic plasmid from a
RT   rumen bacterium of the genus butyrivibrio";
RL   Plasmid 29:63-69(1993).
XX
CC   NM (pRJF1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Butyrivibrio fibrisolvens)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Butyrivibrio fibrisolvens)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Butyrivibrio fibrisolvens
FT                   -> pRJF1 2631bp"
FT   repeat_unit     complement(59..2612)
FT                   /note="RPT inverted, rpt_family is I, putative"
FT   repeat_unit     843..921
FT                   /note="RPT inverted, rpt_family is I, putative"
FT   -35_signal      940..946
FT                   /note="S35 Butyrivibrio replication protein, putative"
FT   -10_signal      963..968
FT                   /note="S10 Butyrivibrio replication protein, putative"
FT   RBS             1028..1033
FT                   /note="RBS Butyrivibrio replication protein SD,
FT                   putative"
FT   rep_origin      1471..1812
FT                   /note="ORI Butyrivibrio replication origin, putative"
FT   CDS             1040..1477
FT                   /note="GEN Butyrivibrio ORF 1, replication protein;
FT                   putative"
FT   CDS             1738..2592
FT                   /note="GEN Butyrivibrio ORF 2; putative"
XX
SQ   Sequence 2631 BP; 908 A; 405 C; 551 G; 767 T; 0 other;
     gaattcactt ggcaaacgta ttttgttgcc tagtgaagtt cgaatctttt tagccgctta
     caaactcttc cagcttgctg gatataatta ataaatcatt tttgatttac ggaagatttt
     aaaaaaagta gctcgaagag cgttgctcaa aattttaatt ttgattgggg attggggata
     ttccccaaca agttttttgc aaaaaaaagc aaatggatat ccatttgccc tgcttgccaa
     gaatgttagt aataaaattg cattttggaa attgtgtggt tctttaaaag aataaaaagg
     gtttcaggga tagaaaaaac gaactagcac tttgttcaga tagcaaagtg ttagttcgtt
     ttttcgtaag ggtcgataga cccttgtagc aagagggtta ggcttcgcct gggggagcgg
     taaagcttcc ccaatgagaa caaggtgagc accctaaacg ggtgcggtgt aagatagctc
     gtcagacgat cttacatacc ttgtatggaa gatgctaaaa attatgctat aatttcaatc
     gttgccaccc ctagactagg cacggaaagg gggtgttagc ttgaagatag ttatctcatt
     tctaatctct gtcgcagcaa gtgtaactgc ctattacatt tgcaaatggc tcgacagcga
     tgataatggc aacgagccta aggattaagc caccttgacc aaaacggaat agaaaaaagc
     taggaactgc cattcctagc tttttttgtt agcttgtcaa aaatagtaat ctcatttctt
     tgcctattct cattatagca agttaattca aatagtcaaa ccagcaagct ggtggtatcc
     tcaagcggct aaaaagattc gaacttcact aggcaacgaa atacgtttgc caagtgaatt
     cgacttttta acctacagcc cgaggatatc taaaaaaact tgttaaacgc ctaattttgg
     catattataa tatataaaaa tgctcatgtt ccatatactg tacttgtacg gtattttgta
     cataaaagtg aggtaaacca tgactattac tgaatttgca gagtcgagac aagtgcaacc
     acaggcaatt tctcgatata taggcaggca tccagaaaaa ttcaacgggc acactgagaa
     aaaaggtaaa acagtagaac ttgatgatat agccttagaa ttgctagaaa agaaatatcc
     tatgcctgcg cctgtacaaa ttatagagga tacagagagc cgtcaaaagc taattaaagc
     gcaagaactg ataatacagc tacaagacaa gctaatggac gcacaaagcc aaattgcaga
     agctgaggca acaaagatac tcttagaaga taaaaacgct caaattgaaa aatacgagct
     tacagaggcg aattacaaaa aacaaattga tgagttatta gaagaattgt cgaaagaaaa
     gtcaaaaacc tggattgata agttgtttaa aaagtgactt tataagagtc actttttttg
     ttgtaaaaat aaataattat ttttcttatt tttattattt taggggaccc gaaagcccgc
     taaagcccgt ggttgcaggg tttttattgt gacctttgga acgttttatt gtgacttttg
     gaacgtttta ttgtgacttt tggaacgttg ataactttta ttgggagatt tagaacgtaa
     ttttattgtg acctttggaa cgtaaatcat ctcctaatgt gacttgaatg tttacgcatg
     agtcacaata tgatattgtt attttcggag ggaataacaa tgaaaaatga cgaccgaatg
     gaaatagcaa ttgaacgtga ttacaaagtt gtaaaggcta atgagattat tcaaaaagca
     aagtttgatt taggattgtt agagcaaaaa actttctgct acgcggtttc aaaaataaag
     cctgatgacg ctttgggcag cgagtatgtt tttacaataa acgaatactg cgatatctgt
     ggaatcaatc gaaatagtgg taaaacaatt gaagaagtta aaagtgcact aaaaagacta
     cgtgataagt ctttttatat gcttgatgaa aaaggagatt atgttctgat tggttggctt
     tcaaaagcaa aagtcagtcc taggagtggc aaaatagcaa tcaagtttga tgaagatatg
     caaaagtatt tgatgggact ttatacaaat tacacccagt atagcttgct ttgtgttcta
     ccaatgcgct cgacatattc tataaggctc tatgaacttt tgaagagcta tgctgggctt
     cacaaaaaag attttgatat agatgatttg aaagctcaac tgtgcgctcc ttatgcaaga
     tttcctgatt ttagaaggaa ggttattgaa gtggcagtaa aagaaattaa tatatataca
     gacatagaag tgtcatggga gccaattaac aaaggacgca aagtggtaaa ggtgcatttt
     agcataaagc agagaaactc aatgaaaatt gcaatggctg gtgcaagagc aacagaagcc
     ttggacggaa tagatggaca aattagattt gatctggaag gtaatttgtc tgaagaaaga
     aaaagtcgct gatccaattt tataagtttg tgggctgtag gttaaaaagt c
//