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ID   PRK101     preliminary; circular DNA; SYN; 9575 BP.
XX
AC   M10567;
XX
DT   15-JUN-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pRK101 - complete, replication origin at R1.
XX
KW   cloning vector;
KW   replication; origin; antibiotic resistance; antibiotic resistance R1.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-318
RC   pRK1030, pRK1031, pRK1032 from pRK101
RC   from pRK103b
RA   Kollek R., Oertel W., Goebel W.;
RT   "Site-specific deletion at the replication origin of the antibiotic
RT   resistance factor R1";
RL   Mol. Gen. Genet. 177:413-419(1980).
XX
RN   [2]
RC   pWL7 from ColE1 & kan gene
RC   pRK101, pRK102, pRK103, pRK107 from pWL812 & pBR322
RC   [pRK103b from pRK103]
RC   pRK104 from pWL812 & pWL7
RA   Kollek R., Oertel W., Goebel W.;
RT   "Isolation and characterization of the minimal fragment required
RT   for autonomous replication (basic replicon) of a copy mutant
RT   (pKN102) of the antibiotic resistance factor R1";
RL   Mol. Gen. Genet. 162:51-57(1978).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NCBI gi: 209123
CC   NM (pRK101)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Rsc13)(pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Rsc13 PstI-PstI 5214bp 2784..7894..104,
FT                   \ ori/amp gene/PstI E/F frags
FT                   \ PstI = 104 646 1189 2784 3404 6253 6911
FT                   -> pWL812 5214bp
FT                   1. pWL812 PstI-PstI 5214bp,
FT                   \ ori/amp gene/PstI E/F frags
FT                   2. pBR322 PstI 4361bp 3612..3612, amp gene
FT                   -> pRK101 9575bp [PstI E only]"
FT   -               1..3611
FT                   /note="pBR322 1..3611 3611bp
FT                   PstI = CTGCA^G"
FT   -               3612..8825
FT                   /note="Rsc13 2784..7894..103 5214bp
FT                   PstI = CTGCA^G"
FT   -               8826..9575
FT                   /note="pBR322 3612..4361 750bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 9575 BP; 2273 A; 2494 C; 2445 G; 2363 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc agtttatgct ggttatgctg gctgcatggg gcattagttc gggagccaga
     tttgtcatgg agcaggccgt tctgctttat ggatcaggaa aaaactattt gttcttcagt
     catggtactg ttctgatgta cctgctgtgt gttttcctgg tatacggggt ctgacgctca
     gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
     tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     attttataaa ccgtggagcg ggcaatactg agctgatgag caatttccgt tgcaccagtg
     cccttctgat gaagcgtcag cacgacgttc ctgtccacgg tacgcctgcg gccaaatttg
     attcctttca gctttgcttc ctgtcggccc tcattcgtgc gctctaggat cctccggcgt
     tcagcctgtg ccacagccga caggatggtg accaccattt gccccatatc accgtcggta
     ctgatcccgt cgtcaataaa ccgaaccgct acaccctgag catcaaactc ttttatcagt
     tggatcatgt cggcggtgtc gcggccaaga cggtcgagct tcttcaccag aatgacatca
     ccttcctcca ccttcatcct cagcaaatcc agcccttccc gatctgttga actgccggat
     gccttgtcgg taaagatgcg gttagctttt acccctgcat ctttgagcgc tctgatctga
     atatcgaggg actgctggct ggttgagacc cgcgcataac caaaaattcg cataaaaatg
     taccttaaat cgaatatcag acacgatgtg tctattatgc caaaatgacg atttaatgga
     cactcaaacg aagccgtttt actatgtctg ataatttata atatttcgaa cggttgcagt
     tgtgttaaaa aagccgtcag gcagggaggc cgatatgccc gttgattttt tgaccactga
     gcaggttgag agttatggca ggttcactgg cgaacccgat gaacttcagc tggcgcgtta
     ttttcatctt gatgaagcgg ataaagaatt tatcgggaaa agccggggtg atcacaatcg
     acttggtatt gccctgcaaa tcgggtgtgt gcgttttctg ggcacttttc ttactgacat
     gaatcatatt ccttccggcg tccggcattt taccgccaga cagctcggga ttcgtgatat
     caccgttctt gcagaatacg gtcagaggga aaatacccgc cgtgagcatg cagcgctgat
     acgtcagcac tatcagtatc gtgaatttgc ctggccctgg acatttcgcc ttacccgtct
     tttatatacc cggagctgga taagcaacga acgtcctggc ctgcttttcg acctggcgac
     agggtggctt atgcaacatc gtattattct ccccggagcc accacgctga cccggttgat
     ttcagaggta agggaaaagg cgacgttgcg cctgtggaac aaactggcac tgataccgtc
     agccgaacag cgttcacagc tggagatgct gctggggcca actgattgca gccgcctgtc
     tttactggaa tcactgaaaa aaggccctgt gaccatcagt ggtccggcgt ttaatgaagc
     aattgaacgc tggaaaactc tgaacgattt tggcctgcat gctgaaaacc tgagtacact
     cccggctgtg cgcctgaaaa atctcgcacg ttatgctggt atgacttcgg tgttcaatat
     tgccaggatg tcaccgcaga aaaggatggc ggttctggtt gcctttgtcc ttgcatggga
     aacgctggcg ctggatgatg cactggacgt tctggacgcc atgctggccg ttatcatccg
     tgacgccaga aagattgggc agaaaaaacg gctccgctcg ctgaaggatc tggataaatc
     tgcattggcg ctcgccagcg catgttcgta cttgctgaaa gaagaaacac cggacgaatc
     gattcgtgct gaggtgttca gctacatccc taggcaaaag ctggctgaaa tcatcacgct
     tgtccgtgaa attgcccggc cctcagacga taattttcat gacgaaatgg tggagcagta
     cgggcgcgtt cgtcgtttcc tgccccatct gctgaatacc gttaaatttt catccgcacc
     tgccggggtt accactctga atgcctgtga ctacctcagc cgggagttca gctcacggcg
     gcagtttttt gacgacgcac caacggaaat catcagtcag tcatggaaac ggctggtgat
     taacaaggaa aaacatatca cccgaagggg atacacgctc tgctttctca gtaaactgca
     ggatagtctg agacggaggg atgtctacgt taccggcagt aaccggtggg gagatcctcg
     tgcaagatta ctacagggtg ctgactggca ggcaaatcgg attaaggttt atcgttcttt
     ggggcacccg acagacccgc aggaagcaat aaaatctctg ggccatcagc ttgatagtcg
     ttacagacag gttgctgcac gtcttggcga aaatgaggct gtcgaactcg atgtttctgg
     cccgaagccc cggttgacaa tttctcccct cgccagtctt gatgagccgg acagtctgaa
     acgactgagc aaaatgatca gtgatctgct ccctccggtg gatttaacgg agttgctgct
     cgaaattaac gcccataccg gatttgctga tgagtttttc catgccagtg aagccagtgc
     cagagttgat gatctgcccg tcagcatcag cgccgtgctg atggctgaag cctgcaatat
     cggtctggaa ccactgatca gatcaaatgt tcctgcactg acccgacacc ggctgaactg
     gacaaaagcg aactatctgc gggctgaaac tatcaccagc gctaatgcca gactggttga
     ttttcaggca acgctgccac tggcacagat atggggtgga ggagaagtgg catctgcaga
     tggaatgcgc tttgttacgc cagtcagaac aatcaatgcc ggaccgaacc gcaaatactt
     tggtaataac agagggatca cctggtacaa ctttgtgtcc gatcagtatt ccggctttca
     tggctttcat ggcatcgtta taccggggac gctgagggac tctatctttg tgctggaagg
     ccttctggaa caggagaccg ggctgaatcc aaccgaaatt atgaccgata cggcaggtgc
     cagcgatctt gtctttggcc ttttctggct gctgggatac cagttttctc cacgcctggc
     tgatgccggt gcttcggttt tctggcgaat ggaccatgat gccgactatg gcgtgctgaa
     tgatattgcc agagggcaat cagatccccg aaaaatagtc cttcagtggg acgaaatgat
     ccggaccgca ggctccctga agctgggcaa agtacaggcc tcagtgctgg tccgttcatt
     gctgaaaagt gaacgtccct ccggactgac tcaggcaatc attgaagtgg ggcgcatcaa
     caaaacgctg tatctgctta attatattga tgatgaagat taccgccggc gcattctgac
     ccagcttaat cggggagaaa gccgtcatgc agttgccaga gccatctgtc acggtcaaaa
     aggtgagata agaaaacgat ataccgacgg tcaggaagat cagttgggag ctctggggct
     ggtcactaac gccgtcgtgt tatggaacac tatttatatg caggcagctc tggatcatct
     ccgggcgcag ggtgaaacac tgaatgatga agatatcgca cggctctccc cgctttgcca
     cggacatatc aatatgctcg gccattattc cttcacgctg gcagaactgg tgaccaaagg
     tcatctgaga ccattaaaag aggcgtcaga ggcagaaaac gttgcttaac gtgagttttc
     gttccactga gcgtcagacc ccatttacga cctgaacgca gacgccggga tattcttctg
     gaagaggttc tgaggcaggc tggtattccg ttgctcagaa gtcacgatgc cagaaaactg
     ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     ataggcgtat cacgaggccc tttcgtcttc aagaa
//