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ID   PRL138     preliminary; circular DNA; SYN; 2740 BP.
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AC   IG5092;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pRL138 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRL19B from pBR322 & Tn5 & piVX & pHC314
RC   pRL447 from pRL19B & pAH484 & pUC8 & pUC18
RC   pRL138, pRL139 from pUC18 & pUC19
RC   pRL148 from pUC18 & linker
RC   pRL171 from pUC18 & pRZ104
RC   pRL178 from pRL19B & pUC18 & pRZ104
RC   pRL424 from pRL19B & pRZ104
RC   pRL425 from pBR322 & pHC314 & pUC18
RC   pRL453 from pRL series
RC   pRL496 from pRL19B & pUC18 & pRZ104
RC   pRL498 from pRL19B & pRZ104
RC   pRL500 from pBR322 & pHC314 & pUC18
RA   Elhai J., Wolk C.P.;
RT   "A versatile class of positive-selection vectors based on the
RT   nonviability of palindrome-containing plasmids that allows cloning
RT   into long polylinkers";
RL   Gene 68:119-138(1988).
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RN   [2]
RC   pAH484 from pBR322 & A.hybridus psbA gene
RC   pAH32S from pBR322 & A.hybridus chloroplast DNA
RA   Hirschberg J., McIntosh L.;
RT   "Molecular basis of herbicide resistance in Amaranthus hybridus";
RL   Science 222:1346-1348(1983).
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RN   [3]
RC   pLacIq from lacIq gene
RA   Wang J.;
RT   ;
RL   Unpublished (1988).
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RN   [4]
RC   pZmc427 from pBR322 & maize psbA gene
RA   Bogorad L., Jolly S.O., Link G., McIntosh L., Poulsen C.,
RA   Schwartz Z., Steinmetz A.;
RT   ;
RL   Biological Chemistry of Organelle Formation 0:87-87(1980).
RL   Bucher T., Sebald W., Weiss H., eds., Springer, Berlin.
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   MCS. not original sequence.
CC   NM (pRL138)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC19)(pUC18)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 ScaI-EcoRI 957bp 2180..2686..451
FT                   2. pUC19 EcoRI-ScaI 1783bp 397..2180
FT                   -> pRL138 2740bp [symmetrical MCS around EcoRI]"
FT   -               1..957
FT                   /note="pUC18 2180..2686..450 957bp
FT                   EcoRI = G^AATTC"
FT   -               958..2740
FT                   /note="pUC19 397..2179 1783bp
FT                   ScaI = AGT^ACT"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 2740 BP; 676 A; 693 C; 702 G; 669 T; 0 other;
     actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     gtgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc gagctcgaat
     tcgagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag cttggcgtaa
     tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     gtcatgccat ccgtaagatg cttttctgtg actggtgagt
//