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ID   PRL271     preliminary; circular DNA; SYN; 6347 BP.
XX
AC   L05081;
XX
DT   05-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Gram-negative bacteria plasmid vector pRL271 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6347
RC   pRL271 from pBR322
RC   pRL277 from pBR322
RC   pRL278 from pBR322
RA   Cai Y.;
RT   "Molecular genetic approaches towards the understanding of
RT   heterocyst differentiation and pattern formation in the
RT   cyanobacterium Anabaena sp.";
RL   Unpublished (1992).
XX
RN   [2]
RC   pRL271 from pBR322
RC   pRL277 from pBR322
RC   pRL278 from pBR322
RA   Black T.A., Cai Y., Wolk C.P.;
RT   "Spatial expression and autoregulation of hetR, a gene involved in
RT   the control of heterocyst development in Anabaena";
RL   Mol. Microbiol. 9:77-84(1993).
XX
CC   vector for sacB-mediated positive selection for double recombinants
CC   in gram-negative bacteria.
CC   NM (pRL271)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(double recombinants)
CC   SE ()
CC   PA (pBR322)
CC   BR (pRL277)(pRL278)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. B. subtilis, sacB gene
FT                   3. pE194, Streptococcus ery gene
FT                   4. Bacillus, cat gene
FT                   -> pRL271 6347bp"
FT   CDS             complement(473..1894)
FT                   /note="GEN B. subtilis sacB gene"
FT   CDS             complement(2393..3127)
FT                   /note="ANT Streptococcus erythromycin resistance gene
FT                   (ery)"
FT   CDS             3503..4162
FT                   /note="ANT Bacillus chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   misc_feature    4252..5221
FT                   /note="MCS"
FT   rep_origin      5222..6347
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322);
FT                   oriV and oriT"
XX
SQ   Sequence 6347 BP; 1649 A; 1305 C; 1404 G; 1989 T; 0 other;
     gctggctgtt ttacgcgtat gacaggctcc ggaagacggt tgttgcgcac gtattcggtg
     aacgcactat ggcgacgctg gggcgtctta tgagcctgct gtcacccttt gacgtggtga
     tatggatgac ggatggctgg ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa
     tcagcaagcg atatacgcag cgaattgagc ggcataacct gaatctgagg cagcacctgg
     cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
     tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc aaaaggttag
     gaatacggtt agccatttgc ctgcttttat atagttcata tgggattcac ctttatgttg
     ataagaaata aaagaaaatg ccaataggat atcggcattt tcttttgcgt ttttatttgt
     taactgttaa ttgtccttgt tcaaggatgc tgtctttgac aacagatgtt ttcttgcctt
     tgatgttcag caggaagctt ggcgcaaacg ttgattgttt gtctgcgtag aatcctctgt
     ttgtcatata gcttgtaatc acgacattgt ttcctttcgc ttgaggtaca gcgaagtgtg
     agtaagtaaa ggttacatcg ttaggatcaa gatccatttt taacacaagg ccagttttgt
     tcagcggctt gtatgggcca gttaaagaat tagaaacata accaagcatg taaatatcgt
     tagacgtaat gccgtcaatc gtcatttttg atccgcggga gtcagtgaac aggtaccatt
     tgccgttcat tttaaagacg ttcgcgcgtt caatttcatc tgttactgtg ttagatgcaa
     tcagcggttt catcactttt ttcagtgtgt aatcatcgtt tagctcaatc ataccgagag
     cgccgtttgc taactcagcc gtgcgttttt tatcgctttg cagaagtttt tgactttctt
     gacggaagaa tgatgtgctt ttgccatagt atgctttgtt aaataaagat tcttcgcctt
     ggtagccatc ttcagttcca gtgtttgctt caaatactaa gtatttgtgg cctttatctt
     ctacgtagtg aggatctctc agcgtatggt tgtcgcctga gctgtagttg ccttcatcga
     tgaactgctg tacattttga tacgtttttc cgtcaccgtc aaagattgat ttataatcct
     ctacaccgtt gatgttcaaa gagctgtctg atgctgatac gttaacttgt gcagttgtca
     gtgtttgttt gccgtaatgt ttaccggaga aatcagtgta gaataaacgg atttttccgt
     cagatgtaaa tgtggctgaa cctgaccatt cttgtgtttg gtcttttagg atagaatcat
     ttgcatcgaa tttgtcgctg tctttaaaga cgcggccagc gtttttccag ctgtcaatag
     aagtttcgcc gactttttga tagaacatgt aaatcgatgt gtcatccgca tttttaggat
     ctccggctaa tgcaaagacg atgtggtagc cgtgatagtt tgcgacagtg ccgtcagcgt
     tttgtaatgg ccagctgtcc caaacctcca ggccttttgc agaagagata tttttaattg
     tggacgaatc gaattcagga acttgatatt tttcattttt ttgctgttca gggatttgca
     gcatatcatg gcgtgtaata tgggaaatgc cgtatgtttc cttatatggc ttttggttcg
     tttctttcgc aaacgcttga gttgcgcctc ctgccagcag tgcggtagta aaggttaata
     ctgttgcttg ttttgcaaac tttttgatgt tcatcgttca tgtctccttt tttatgtact
     gtgttagcgg tctgcttctt ccagccctcc tgtttgaaga tggcaagtta gttacgcaca
     ataaaaaaag acctaaaata tgtaaggggt gacgccaaag tatacacttt gccctttaca
     cattttaggt cttgcctgct ttatcagtaa caaacccgcg cgatttactt ttcgacctca
     ttctattaga ctctcgtttg gattgcaact ggtctatttt cctcttttgt ttgatagaaa
     atcataaaag gatttgcaga ctacgggcct aaagaactaa aaaatctatc tgtttctttt
     cattctctgt attttttata gtttctgttg catgggcata aagttgcctt tttaatcaca
     attcagaaaa tatcataata tctcatttca ctaaataata gtgaacggca ggtatatgtg
     atgggttaaa aaggatcgat cctctagcta gagtcgacct gcatccctta acttacttat
     taaataattt atagctattg aaaagagata agaattgttc aaagctaata ttgtttaaat
     cgtcaattcc tgcatgtttt aaggaattgt taaattgatt ttttgtaaat attttcttgt
     attctttgtt aacccatttc ataacgaaat aattatactt ttgtttatct ttgtgtgata
     ttcttgattt ttttctactt aatctgataa gtgagctatt cactttaggt ttaggatgaa
     aatattctct tggaaccata cttaatatag aaatatcaac ttctgccatt aaaagtaatg
     ccaatgagcg ttttgtattt aataatcttt tagcaaaccc gtattccacg attaaataaa
     tctcattagc tatactatca aaaacaattt tgcgtattat atccgtactt atgttataag
     gtatattacc atatatttta taggattggt ttttaggaaa tttaaactgc aatatatcct
     tgtttaaaac ttggaaatta tcgtgatcaa caagtttatt ttctgtagtt ttgcataatt
     tatggtctat ttcaatggca gttacgaaat tacacctctt tactaattca agggtaaaat
     ggccttttcc tgagccgatt tcaaagatat tatcatgttc atttaatctt atatttgtca
     ttattttatc tatattatgt tttgaagtaa taaagttttg actgtgtttt atatttttct
     cgttcattat aaccctcttt aatttggtta tatgaatttt gcttattaac gattcattat
     aaccacttat tttttgtttg gttgataatg aactgtgctg attacaaaaa tactaaaaat
     gcccatattt tttcctcctt ataaaattag tataattata gcacgcgaat tcatcgaata
     aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
     cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
     caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
     ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
     tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgat atctagatct
     cgagctggat acttcccgtc cgccaggggg acatgccggc gatgctgaag gtcgcgcgca
     ttcccgatga agaggccggt taccgcctgt ttgaggatat agtaatcttt ctaaatagct
     ttggattgga ggagtatggc cactaatact aagttcagct aataaaaaaa tttgctaaag
     aactccagct ggatttcact gatgagaata tcgtcggaga taaatataat aattccacgg
     actatagact atactagtat actccgtcta ctgtacgata cacttccgct caggtccttg
     tcctttaacg aggattgtta cgtacgctaa tggcgtcaaa acaaagactc tagacctagg
     ccttaagatc gaaagagctg gtagttggcg cactgttcga agaactgccg atgtccagta
     agattcttac tatgctggtt gaaccggatg ctggtaaagc tacttgggtt gctgcttcta
     cttatggtac cgatacaact actggtgagg aagttaaagg agctcgcgaa agcttgcatg
     cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcag
//