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ID   PRL277     preliminary; circular DNA; SYN; 6798 BP.
XX
AC   L05082;
XX
DT   05-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Gram-negative bacteria plasmid vector pRL277 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6798
RC   pRL271 from pBR322
RC   pRL277 from pBR322
RC   pRL278 from pBR322
RA   Cai Y.;
RT   "Molecular genetic approaches towards the understanding of
RT   heterocyst differentiation and pattern formation in the
RT   cynobacterium Anabaena sp.";
RL   Unpublished (1992).
XX
RN   [2]
RC   pRL271 from pBR322
RC   pRL277 from pBR322
RC   pRL278 from pBR322
RA   Black T.A., Cai Y., Wolk C.P.;
RT   "Spatial expression and autoregulation of hetR, a gene involved in
RT   the control of heterocyst development in Anabaena";
RL   Mol. Microbiol. 9:77-84(1993).
XX
CC   vector for sacB-mediated positive selection for double recombinants
CC   in gram-negative bacteria.
CC   NM (pRL277)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(double recombinants)
CC   SE ()
CC   PA (pBR322)
CC   BR (pRL271)(pRL278)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. B. subtilis, sacB gene
FT                   3. pS194, spec/strep genes
FT                   -> pRL277 6798bp"
FT   CDS             complement(473..1894)
FT                   /note="GEN B. subtilis sacB gene"
FT   CDS             complement(2980..3771)
FT                   /note="ANT Bacillus streptomycin resistance gene
FT                   (str)"
FT   CDS             complement(2980..3771)
FT                   /note="ANT Bacillus spectinomycin resistance gene
FT                   (spc); aadA"
FT   misc_feature    4241..5504
FT                   /note="MCS"
FT   rep_origin      5667..6798
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322);
FT                   oriV and oriT"
XX
SQ   Sequence 6798 BP; 1653 A; 1540 C; 1637 G; 1968 T; 0 other;
     gctggctgtt ttacgcgtat gacaggctcc ggaagacggt tgttgcgcac gtattcggtg
     aacgcactat ggcgacgctg gggcgtctta tgagcctgct gtcacccttt gacgtggtga
     tatggatgac ggatggctgg ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa
     tcagcaagcg atatacgcag cgaattgagc ggcataacct gaatctgagg cagcacctgg
     cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
     tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc aaaaggttag
     gaatacggtt agccatttgc ctgcttttat atagttcata tgggattcac ctttatgttg
     ataagaaata aaagaaaatg ccaataggat atcggcattt tcttttgcgt ttttatttgt
     taactgttaa ttgtccttgt tcaaggatgc tgtctttgac aacagatgtt ttcttgcctt
     tgatgttcag caggaagctt ggcgcaaacg ttgattgttt gtctgcgtag aatcctctgt
     ttgtcatata gcttgtaatc acgacattgt ttcctttcgc ttgaggtaca gcgaagtgtg
     agtaagtaaa ggttacatcg ttaggatcaa gatccatttt taacacaagg ccagttttgt
     tcagcggctt gtatgggcca gttaaagaat tagaaacata accaagcatg taaatatcgt
     tagacgtaat gccgtcaatc gtcatttttg atccgcggga gtcagtgaac aggtaccatt
     tgccgttcat tttaaagacg ttcgcgcgtt caatttcatc tgttactgtg ttagatgcaa
     tcagcggttt catcactttt ttcagtgtgt aatcatcgtt tagctcaatc ataccgagag
     cgccgtttgc taactcagcc gtgcgttttt tatcgctttg cagaagtttt tgactttctt
     gacggaagaa tgatgtgctt ttgccatagt atgctttgtt aaataaagat tcttcgcctt
     ggtagccatc ttcagttcca gtgtttgctt caaatactaa gtatttgtgg cctttatctt
     ctacgtagtg aggatctctc agcgtatggt tgtcgcctga gctgtagttg ccttcatcga
     tgaactgctg tacattttga tacgtttttc cgtcaccgtc aaagattgat ttataatcct
     ctacaccgtt gatgttcaaa gagctgtctg atgctgatac gttaacttgt gcagttgtca
     gtgtttgttt gccgtaatgt ttaccggaga aatcagtgta gaataaacgg atttttccgt
     cagatgtaaa tgtggctgaa cctgaccatt cttgtgtttg gtcttttagg atagaatcat
     ttgcatcgaa tttgtcgctg tctttaaaga cgcggccagc gtttttccag ctgtcaatag
     aagtttcgcc gactttttga tagaacatgt aaatcgatgt gtcatccgca tttttaggat
     ctccggctaa tgcaaagacg atgtggtagc cgtgatagtt tgcgacagtg ccgtcagcgt
     tttgtaatgg ccagctgtcc caaacctcca ggccttttgc agaagagata tttttaattg
     tggacgaatc gaattcagga acttgatatt tttcattttt ttgctgttca gggatttgca
     gcatatcatg gcgtgtaata tgggaaatgc cgtatgtttc cttatatggc ttttggttcg
     tttctttcgc aaacgcttga gttgcgcctc ctgccagcag tgcggtagta aaggttaata
     ctgttgcttg ttttgcaaac tttttgatgt tcatcgttca tgtctccttt tttatgtact
     gtgttagcgg tctgcttctt ccagccctcc tgtttgaaga tggcaagtta gttacgcaca
     ataaaaaaag acctaaaata tgtaaggggt gacgccaaag tatacacttt gccctttaca
     cattttaggt cttgcctgct ttatcagtaa caaacccgcg cgatttactt ttcgacctca
     ttctattaga ctctcgtttg gattgcaact ggtctatttt cctcttttgt ttgatagaaa
     atcataaaag gatttgcaga ctacgggcct aaagaactaa aaaatctatc tgtttctttt
     cattctctgt attttttata gtttctgttg catgggcata aagttgcctt tttaatcaca
     attcagaaaa tatcataata tctcatttca ctaaataata gtgaacggca ggtatatgtg
     atgggttaaa aaggatcgat cctctagcta gagtcgacct gcatccctta acttacttat
     taaataattt atagctattg aaaagagata agaattgttc aaagctaata ttgtttaaat
     cgtcaattcc tgcatgtttt aaggaattgt taaattgatt ttttgtaaat attttcttgt
     attctttgtt aacccatttc ataacgaaat aattatactt ttgtttatct ttgtgtgata
     ttcttgattt ttttctactt aatctgataa gtgagctatt cactttaggt ttaggatgaa
     aaaaaataaa aaaggggacc tctagggtcc ccaattaatt agtaatataa tctattaaag
     gtcattcaaa aggtcatcca ccggatcagc ttagtaaagc cctcgctaga ttttaatgcg
     gatgttgcga ttacttcgcc aactattgcg ataacaagaa aaagccagcc tttcatgata
     tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa gcagacttga
     cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcttg agttaagccg
     cgccgcgaag cggcgtcggc ttgaacgaat tgttagacat tatttgccga ctaccttggt
     gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
     atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
     cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
     tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
     gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
     aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
     ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
     aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
     aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
     aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
     ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
     tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
     aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca tgatgtttaa
     ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
     tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa
     aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
     caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct tacgaaccga
     acaggcttat gtccactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc
     aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt
     ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct
     gtgcacggat ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc
     ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg
     tttgttcgcc cagcttctgt atggaacggg catgcggatc agtgagggtt tgcaactgcg
     ggtcaaggat ctggatttcg atcacggcac gatcatcgtg cgggagggca agggctccaa
     ggatcgggcc ttgatgttac ccgagagctt ggcacccagc ctgcgcgagc aggggaattg
     atccggtgga tgaccttttg aatgaccttt aatagattat attactaatt aattggggac
     cctagaggtc ccctttttta ttttactgcg atgagtggca gggcggggcg taattttttt
     aaggcagtta ttggtgccct taaacgcctg gtgctacgcc tgaataagtg ataataagcg
     gatgaatggc agaaattcga tatctagatc tcgagctgga tacttcccgt ccgccagggg
     gacatgccgg cgatgctgaa ggtcgcgcgc attcccgatg aagaggccgg ttaccgcctg
     tttgaggata tagtaatctt tctaaatagc tttggattgg aggagtatgg ccactaatac
     taagttcagc taataaaaaa atttgctaaa gaactccagc tggatttcac tgatgagaat
     atcgtcggag ataaatataa taattccacg gactatagac tatactagta tactccgtct
     actgtacgat acacttccgc tcaggtcctt gtcctttaac gaggattgtt acgtacgcta
     atggcgtcaa aacaaagact ctagacctag gccttaagat cgaaagagct ggtagttggc
     gcactgttcg aagaactgcc gatgtccagt aagattctta ctatgctggt tgaaccggat
     gctggtaaag ctacttgggt tgctgcttct acttatggta ccgatacaac tactggtgag
     gaagttaaag gagctcgcga aagcttgcat gcctgcagca atggcaacaa cgttgcgcaa
     actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     ccgaaacgcg cgaggcag
//