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ID   PRL278     preliminary; circular DNA; SYN; 5941 BP.
XX
AC   L05083;
XX
DT   05-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Gram-negative bacteria plasmid vector pRL278 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-5941
RC   pRL271 from pBR322
RC   pRL277 from pBR322
RC   pRL278 from pBR322
RA   Cai Y.;
RT   "Molecular genetic approaches towards the understanding of
RT   heterocyst differentiation and pattern formation in the
RT   cynobacterium Anabaena sp.";
RL   Unpublished (1992).
XX
RN   [2]
RC   pRL271 from pBR322
RC   pRL277 from pBR322
RC   pRL278 from pBR322
RA   Black T.A., Cai Y., Wolk C.P.;
RT   "Spatial expression and autoregulation of hetR, a gene involved in
RT   the control of heterocyst development in Anabaena";
RL   Mol. Microbiol. 9:77-84(1993).
XX
CC   NM (pRL278)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(double recombinants)
CC   SE ()
CC   PA (pBR322)
CC   BR (pRL271)(pRL277)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. B. subtilis, sacB gene
FT                   3. Bacillus, kan gene
FT                   -> pRL278 5941bp"
FT   CDS             complement(473..1894)
FT                   /note="GEN B. subtilis sacB gene"
FT   CDS             2749..3543
FT                   /note="ANT Bacillus neomycin phosphotransferase II
FT                   gene (NPTII), neomycin resistance gene (neo)
FT                   kanamycin resistance gene (kan)"
FT   misc_feature    3558..4815
FT                   /note="MCS"
FT   rep_origin      4816..5941
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322);
FT                   oriV and oriT"
XX
SQ   Sequence 5941 BP; 1419 A; 1329 C; 1471 G; 1722 T; 0 other;
     gctggctgtt ttacgcgtat gacaggctcc ggaagacggt tgttgcgcac gtattcggtg
     aacgcactat ggcgacgctg gggcgtctta tgagcctgct gtcacccttt gacgtggtga
     tatggatgac ggatggctgg ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa
     tcagcaagcg atatacgcag cgaattgagc ggcataacct gaatctgagg cagcacctgg
     cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
     tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc aaaaggttag
     gaatacggtt agccatttgc ctgcttttat atagttcata tgggattcac ctttatgttg
     ataagaaata aaagaaaatg ccaataggat atcggcattt tcttttgcgt ttttatttgt
     taactgttaa ttgtccttgt tcaaggatgc tgtctttgac aacagatgtt ttcttgcctt
     tgatgttcag caggaagctt ggcgcaaacg ttgattgttt gtctgcgtag aatcctctgt
     ttgtcatata gcttgtaatc acgacattgt ttcctttcgc ttgaggtaca gcgaagtgtg
     agtaagtaaa ggttacatcg ttaggatcaa gatccatttt taacacaagg ccagttttgt
     tcagcggctt gtatgggcca gttaaagaat tagaaacata accaagcatg taaatatcgt
     tagacgtaat gccgtcaatc gtcatttttg atccgcggga gtcagtgaac aggtaccatt
     tgccgttcat tttaaagacg ttcgcgcgtt caatttcatc tgttactgtg ttagatgcaa
     tcagcggttt catcactttt ttcagtgtgt aatcatcgtt tagctcaatc ataccgagag
     cgccgtttgc taactcagcc gtgcgttttt tatcgctttg cagaagtttt tgactttctt
     gacggaagaa tgatgtgctt ttgccatagt atgctttgtt aaataaagat tcttcgcctt
     ggtagccatc ttcagttcca gtgtttgctt caaatactaa gtatttgtgg cctttatctt
     ctacgtagtg aggatctctc agcgtatggt tgtcgcctga gctgtagttg ccttcatcga
     tgaactgctg tacattttga tacgtttttc cgtcaccgtc aaagattgat ttataatcct
     ctacaccgtt gatgttcaaa gagctgtctg atgctgatac gttaacttgt gcagttgtca
     gtgtttgttt gccgtaatgt ttaccggaga aatcagtgta gaataaacgg atttttccgt
     cagatgtaaa tgtggctgaa cctgaccatt cttgtgtttg gtcttttagg atagaatcat
     ttgcatcgaa tttgtcgctg tctttaaaga cgcggccagc gtttttccag ctgtcaatag
     aagtttcgcc gactttttga tagaacatgt aaatcgatgt gtcatccgca tttttaggat
     ctccggctaa tgcaaagacg atgtggtagc cgtgatagtt tgcgacagtg ccgtcagcgt
     tttgtaatgg ccagctgtcc caaacctcca ggccttttgc agaagagata tttttaattg
     tggacgaatc gaattcagga acttgatatt tttcattttt ttgctgttca gggatttgca
     gcatatcatg gcgtgtaata tgggaaatgc cgtatgtttc cttatatggc ttttggttcg
     tttctttcgc aaacgcttga gttgcgcctc ctgccagcag tgcggtagta aaggttaata
     ctgttgcttg ttttgcaaac tttttgatgt tcatcgttca tgtctccttt tttatgtact
     gtgttagcgg tctgcttctt ccagccctcc tgtttgaaga tggcaagtta gttacgcaca
     ataaaaaaag acctaaaata tgtaaggggt gacgccaaag tatacacttt gccctttaca
     cattttaggt cttgcctgct ttatcagtaa caaacccgcg cgatttactt ttcgacctca
     ttctattaga ctctcgtttg gattgcaact ggtctatttt cctcttttgt ttgatagaaa
     atcataaaag gatttgcaga ctacgggcct aaagaactaa aaaatctatc tgtttctttt
     cattctctgt attttttata gtttctgttg catgggcata aagttgcctt tttaatcaca
     attcagaaaa tatcataata tctcatttca ctaaataata gtgaacggca ggtatatgtg
     atgggttaaa aaggatcgat cctctagcta gagtcgacct gcatccctta acttacttat
     taaataattt atagctattg aaaagagata agaattgttc aaagctaata ttgtttaaat
     cgtcaattcc tgcatgtttt aaggaattgt taaattgatt ttttgtaaat attttcttgt
     attctttgtt aacccatttc ataacgaaat aattatactt ttgtttatct ttgtgtgata
     ttcttgattt ttttctactt aatctgataa gtgagctatt cactttaggt ttaggatgaa
     aactagagga tctcaatgaa tattggttga cacgggcgta taagacatgt tatactgttg
     aataacaagg acggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccggggat
     cctctagact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc
     ccttaaacgc ctggtgctac gcctgaataa gtgataataa gcggatgaat ggcagaaatt
     cgatatctag atctcgagct ggatacttcc cgtccgccag ggggacatgc cggcgatgct
     gaaggtcgcg cgcattcccg atgaagaggc cggttaccgc ctgtttgagg atatagtaat
     ctttctaaat agctttggat tggaggagta tggccactaa tactaagttc agctaataaa
     aaaatttgct aaagaactcc agctggattt cactgatgag aatatcgtcg gagataaata
     taataattcc acggactata gactatacta gtatactccg tctactgtac gatacacttc
     cgctcaggtc cttgtccttt aacgaggatt gttacgtacg ctaatggcgt caaaacaaag
     actctagacc taggccttaa gatcgaaaga gctggtagtt ggcgcactgt tcgaagaact
     gccgatgtcc agtaagattc ttactatgct ggttgaaccg gatgctggta aagctacttg
     ggttgctgct tctacttatg gtaccgatac aactactggt gaggaagtta aaggagctcg
     cgaaagcttg catgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt
     gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     g
//