back Return to this vector's summary.
ID   PRS200     preliminary; circular DNA; SYN; 4793 BP.
XX
AC   ATCC77165;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli phagemid vector pRS200 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRSS56 from pBluescript KS+ & BlueScribe
RC   pRS303 from pRSS56 & HIS3 gene
RC   pRS304 from pRSS56 & TRP1 gene
RC   pRS305 from pRSS56 & LEU2 gene
RC   pRS306 from pRSS56 & URA3 gene
RC   pUC19-CEN6:32 from pUC19 & CEN6
RC   pRSS83 from pUC19-CEN6:32 & pAB9, ARS
RC   pRSS84 from pRSS83
RC   pRS313 from pRS303 & pRSS84
RC   pRS314 from pRS304 & pRSS84
RC   pRS315 from pRS305 & pRSS84
RC   pRS316 from pRS306 & pRSS84
RC   pRSS93 from pRSS84 & pYCF5
RA   Sikorski R.S., Hieter P.;
RT   "A system of shuttle vectors and yeast host strains designed for
RT   efficient manipulation of DNA in Saccharomyces cerevisiae";
RL   Genetics 122:19-27(1989).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Modified from pRS314 (ATCC 77143) by replacing the SmaI site in the
CC   MCS with BglII linkers.  Permits double digestion of the vector to
CC   lower background when inserting Sau3AI fragments. (personal
CC   communication)
CC   YC-type centromere vector permitting visual detection of recombinants
CC   and production of ssDNA in E.coli.  Contains promoters for in vitro
CC   RNA synthesis, priming sites useful for sequencing, and encodes the
CC   lacZ alpha (lacZ') peptide.
CC   pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of
CC   pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains
CC   the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites
CC   between bla and f1 origin of pBS(+).
CC   The order of the major features in this plasmid is: TRP1 - f1 ori
CC   (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla - CEN6
CC   - ARSH4.
CC   An EcoRI site in the TRP1-containing fragment (external to the coding
CC   sequence) was destroyed.
CC   Restriction digests of the clone give the following sizes (kb):
CC   PvuI--2.8, 2.0; HindIII--3.7, 1.1; EcoRI--3.5, 1.3; XbaI--3.4, 1.4;
CC   PvuII--4.3; 0.4. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pRS200)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pRS314)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pRS314 SmaI 4783bp 1934..1934, MCS
FT                   BglII linker 6bp agatct
FT                   -> plasmid 4790bp
FT                   1. plasmid EcoRI 4790bp 1944..1944, TRP1 IVS
FT                   fill in
FT                   -> pRS200 4785bp"
FT   -               1..1933
FT                   /note="pRS314 1..1933 1933bp
FT                   SmaI = XmaI = CCC^GGG
FT                   \                 agatct"
FT   -               1934..1939
FT                   /note="agatct 6bp
FT                   \          agatct
FT                   SmaI = XmaI = CCC^GGG"
FT   -               1940..1953
FT                   /note="pRS314 1934..1947 14bp
FT                   EcoRI = G^AATT C
FT                   EcoRI =      G^AATTC"
FT   -               1954..4793
FT                   /note="pRS314 1944..4783 2840bp"
FT   misc_binding    0..0
FT                   /note="MCS KpnI-ApaI-XhoI-SalI-ClaI-HindIII-EcoRV-
FT                   EcoRI-PstI-BglII-
FT                   BamHI-SpeI-XbaI-EagI-NotI-BstXI-SacII-SacI"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI-ApaI-XhoI-SalI-ClaI-EcoRI-
FT                   PstI-BglII-BamHI-SpeI-EagI-NotI-SacII-SacI-NaeI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS H4"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN6"
XX
SQ   Sequence 4793 BP; 1313 A; 1048 C; 1154 G; 1278 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
     atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
     aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
     gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
     tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
     atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
     cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
     agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
     atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
     ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
     ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
     actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
     attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
     gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgcctg cggtgtgaaa
     taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt
     gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     gctacagggc gcgtcgcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     ggtgcgggcc tcttcgctat tacgccagct ggcgaagggg ggatgtgctg caaggcgatt
     aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctctagaac
     tagtggatcc cccagatctg ggctgcagga attaattcga tatcaagctt atcgataccg
     tcgacctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg gttaattccg
     agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     ccacacaaca taggagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagg
     taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     ttccataggc tcggcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     cgaaacccga caggactata aagataccag gcgttccccc ctggaagctc cctcgtgcgc
     tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact gcccgtcgtg
     tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     aggcgagtta catgatcccc catgttgtga aaaaaagcgg ttagctcctt cggtcctccg
     atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     gtgccacctg ggtccttttc atcacgtgct ataaaaataa ttataattta aattttttaa
     tataaatata taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt
     gttttccgaa gatgtaaaag actctagggg gatcgccaac aaatactacc ttttatcttg
     ctcttcctgc tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac
     acacgaaaat cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa
     tctgcttttc ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa
     acctttgttt attttttttt cttcattccg taactcttct accttcttta tttactttct
     aaaatccaaa tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca
     tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc gatccgtcct aagaaaccat
     tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//