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ID PRS303 preliminary; circular DNA; SYN; 4443 BP.
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AC U03435; ATCC77138;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Saccharomyces/E.coli phagemid vector pRS303 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-4443
RC pRSS56 from pBluescript KS+ & BlueScribe
RC pRS303 from pRSS56 & HIS3 gene
RC pRS304 from pRSS56 & TRP1 gene
RC pRS305 from pRSS56 & LEU2 gene
RC pRS306 from pRSS56 & URA3 gene
RC pUC19-CEN6:32 from pUC19 & CEN6
RC pRSS83 from pUC19-CEN6:32 & pAB9, ARS
RC pRSS84 from pRSS83
RC pRS313 from pRS303 & pRSS84
RC pRS314 from pRS304 & pRSS84
RC pRS315 from pRS305 & pRSS84
RC pRS316 from pRS306 & pRSS84
RC pRSS93 from pRSS84 & pYCF5
RA Sikorski R.S., Hieter P.;
RT "A system of shuttle vectors and yeast host strains designed for
RT efficient manipulation of DNA in Saccharomyces cerevisiae";
RL Genetics 122:19-27(1989).
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RN [2]
RC pYCp19 from pBR322 & CEN4/ARS1/TRP1/URA3
RA Mann C., Davis R.;
RT ;
RL Unpublished (1989).
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RN [3]
RC pRS303
RA Stillman D.J.;
RT ;
RL Submitted (10-NOV-1993) by:
RL Stillman D.J., Dept. of Cellular, Viral and Molecular Biology,
RL University of Utah Medical Center, Salt Lake City, UT 84132 USA.
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CC pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of
CC pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains
CC the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites
CC between bla and f1 origin of pBS(+).
CC A fragment (1.184 kb) containing the HIS3 gene was inserted into the
CC NdeI site of pRSS56. All ends were blunted.
CC One of a series of pBluescript-based integrating vectors (ATCC
CC 77138-77141) differing in the yeast selectable marker gene.
CC YI-type integrating shuttle vector permitting visual detection of
CC recombinants & production of ssDNA in E.coli. Contains promoters for
CC in vitro RNA synthesis, priming sites useful for sequencing, and
CC encodes the lacZ alpha (lacZ') peptide.
CC The order of the major features in this plasmid is: HIS3 - f1 ori
CC (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla.
CC Restriction digests of the clone give the following sizes (kb):
CC HindIII--3.2, 1.1, 0.24; PvuII--4.0, 0.48; XbaI--4.5; EcoRI--4.5.
CC (ATCC staff)
CC Medium is 1227 LB plus ampicillin.
CC NCBI gi: 416304
CC NM (pRS303)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (ATCC)
CC HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC HO (Saccharomyces cerevisiae YPH499)(Saccharomyces cerevisiae YPH500)
CC HO (Saccharomyces cerevisiae YPH501)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR (pRS304)(pRS305)(pRS306)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBluescript KS+ PvuI-PvuI 1914bp 498..2412,
FT \ MCS
FT 2. pBS+ PvuI-PvuI 1084bp 2850..3204..730, amp/f1 ori
FT -> pRSS56 2998bp [unique NdeI, unique AatII]
FT 1. pRSS56 NdeI 2998bp 185..185, pBS+
FT Klenow:Klenow
FT 2. yeast, -296 of HIS3 gene/pYAC4 5839..5839
FT \ pYAC4 1768bp 4238..6006/CDS = complement(4884..5543)
FT EcoRI linker 6bp gaattc
FT XhoI, end of HIS3 gene/pYAC4 4677..4677
FT BamHI linker 6bp ggatcc
FT EcoRI-BamHI 1184bp, HIS3 gene/4671..5845
FT Klenow:Klenow
FT -> pRS303 4443bp"
FT misc_binding 0..0
FT /note="MCS KpnI-ApaI-XhoI-ClaI-HindIII-EcoRV-EcoRI-
FT PstI-SmaI-BamHI-SpeI-XbaI-EagI-NotI-BstXI-SacII-SacI"
FT misc_binding 0..0
FT /note="SIT unique ApaI-XhoI-SalI-ClaI-EcoRV-EcoRI-
FT SmaI-BamHI-SpeI-XbaI-EagI-NotI-SacII-SacI-NaeI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
FT promoter 0..0
FT /note="PRO E. coli lac gene"
FT promoter 0..0
FT /note="PRO bacteriophage T3"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ');
FT reporter gene"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="ANT yeast HIS3 gene"
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SQ Sequence 4443 BP; 1149 A; 1048 C; 1109 G; 1137 T; 0 other;
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga
acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt
actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt
ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa
tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca
ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa
tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc
gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat
taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc
cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac
tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctactgg cgcgtggagt
aaaaaggttt ggatcaggat ttgcgccttt ggatgaggca ctttccagag cggtggtaga
tctttcgaac aggccgtacg cagttgtcga acttggtttg caaagggaga aagtaggaga
tctctcttgc gagatgatcc cgcattttct tgaaagcttt gcagaggcta gcagaattac
cctccacgtt gattgtctgc gaggcaagaa tgatcatcac cgtagtgaga gtgcgttcaa
ggctcttgcg gttgccataa gagaagccac ctcgcccaat ggtaccaacg atgttccctc
caccaaaggt gttcttatgt agtgacaccg attatttaaa gctgcagcat acgatatata
tacatgtgta tatatgtata cctatgaatg tcagtaagta tgtatacgaa cagtatgata
ctgaagatga caaggtaatg catcattcta tacgtgtcat tctgaacgag gcgcgctttc
cttttttctt tttgcttttt cttttttttt ctcttgaact cgacggatca tatgcggtgt
gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa ggggggatgt gctgcaaggc
gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
aattgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta
gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc
tcgagggggg gcccggtacc cagcttttgt tccctttagt gagggttaat tccgagcttg
gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
aacataggag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc
acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
aggctcggcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
ccgacaggac tataaagata ccaggcgttc ccccctggaa gctccctcgt gcgctctcct
gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
atctcagcga tctgtctatt tcgttcatcc atagttgcct gactgcccgt cgtgtagata
actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
gttacatgat cccccatgtt gtgaaaaaaa gcggttagct ccttcggtcc tccgatcgtt
gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
cctgacgtct tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
gtc
//