back Return to this vector's summary.
ID   PRS306     preliminary; circular DNA; SYN; 4373 BP.
XX
AC   U03438; ATCC77141;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli phagemid vector pRS306 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4373
RC   pRSS56 from pBluescript KS+ & BlueScribe
RC   pRS303 from pRSS56 & HIS3 gene
RC   pRS304 from pRSS56 & TRP1 gene
RC   pRS305 from pRSS56 & LEU2 gene
RC   pRS306 from pRSS56 & URA3 gene
RC   pUC19-CEN6:32 from pUC19 & CEN6
RC   pRSS83 from pUC19-CEN6:32 & pAB9, ARS
RC   pRSS84 from pRSS83
RC   pRS313 from pRS303 & pRSS84
RC   pRS314 from pRS304 & pRSS84
RC   pRS315 from pRS305 & pRSS84
RC   pRS316 from pRS306 & pRSS84
RC   pRSS93 from pRSS84 & pYCF5
RA   Sikorski R.S., Hieter P.;
RT   "A system of shuttle vectors and yeast host strains designed for
RT   efficient manipulation of DNA in Saccharomyces cerevisiae";
RL   Genetics 122:19-27(1989).
XX
RN   [2]
RC   pRS306
RA   Stillman D.J.;
RT   ;
RL   Submitted (10-NOV-1993) by:
RL   Stillman D.J., Dept. of Cellular, Viral and Molecular Biology,
RL   University of Utah Medical Center, Salt Lake City, UT 84132 USA.
XX
CC   One of a series of pBluescript-based integrating vectors (ATCC
CC   77138-77141) differing in the yeast selectable marker gene.
CC   The order of the major features in this plasmid is: URA3 - f1 ori
CC   (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla.
CC   YI-type integrating shuttle vector permitting visual detection of
CC   recombinants and production of ssDNA in E.coli. Contains promoters for
CC   in vitro RNA synthesis, priming sites useful for sequencing, and
CC   encodes the lacZ alpha (lacZ') peptide.
CC   pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of
CC   pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains
CC   the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites
CC   between bla and f1 origin of pBS(+).
CC   A fragment (1.112 kb) containing the URA3 gene was inserted into the
CC   NdeI site of pRSS56.  All ends were blunted.
CC   Restriction digests of the clone give the following sizes (kb):
CC   PstI--2.7, 1.65; PvuI--3.9, 0.40; EcoRI--4.4; HindIII--4.4. (ATCC
CC   staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 416307
CC   NM (pRS306)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae YPH499)(Saccharomyces cerevisiae YPH500)
CC   HO (Saccharomyces cerevisiae YPH501)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR (pRS304)(pRS305)(pRS303)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBluescript KS+ PvuI-PvuI 1914bp 498..2412,
FT                   \ MCS
FT                   2. pBS+ PvuI-PvuI 1084bp 2850..3204..730, amp/f1 ori
FT                   -> pRSS56 2998bp [unique NdeI, unique AatII]
FT                   1. pRSS56 NdeI 2998bp 185..185, pBS+
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   2. YEp24 HindIII 2273..2273, URA3 gene
FT                   SacI linker 6bp gagctc
FT                   SacI-SmaI 1112bp 2269..3381, URA3 gene
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pRS306 4373bp"
FT   misc_binding    0..0
FT                   /note="MCS KpnI-ApaI-XhoI-SalI-ClaI-HindIII-EcoRV-
FT                   EcoRI-PstI-SmaI-
FT                   BamHI-SpeI-XbaI-EagI-NotI-BstXI-SacII-SacI"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI-XhoI-SalI-ClaI-HindIII-
FT                   EcoRI-SmaI-BamHI-SpeI-XbaI-EagI-
FT                   NotI-BstXI-SacII-SacI-NaeI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
XX
SQ   Sequence 4373 BP; 1181 A; 987 C; 1106 G; 1099 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
     tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
     gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
     tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
     ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
     tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
     tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
     tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
     acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
     agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
     tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
     ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
     atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
     ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
     aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
     aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
     cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca
     gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcccccggg
     ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc
     cagcttttgt tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct
     gtttcctgtg tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat
     aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc
     actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctcggcc cccctgacga
     gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     ccaggcgttc ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     tcgttcatcc atagttgcct gactgcccgt cgtgtagata actacgatac gggagggctt
     accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     gtgaaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//