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ID   PRS403     preliminary; circular DNA; SYN; 4456 BP.
XX
AC   U03443; IG3011;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli phagemid vector pRS403 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4456
RC   pRSS56 from pBluescript KS+ & BlueScribe
RC   pRS303 from pRSS56 & HIS3 gene
RC   pRS304 from pRSS56 & TRP1 gene
RC   pRS305 from pRSS56 & LEU2 gene
RC   pRS306 from pRSS56 & URA3 gene
RC   pUC19-CEN6:32 from pUC19 & CEN6
RC   pRSS83 from pUC19-CEN6:32 & pAB9, ARS
RC   pRSS84 from pRSS83
RC   pRS313 from pRS303 & pRSS84
RC   pRS314 from pRS304 & pRSS84
RC   pRS315 from pRS305 & pRSS84
RC   pRS316 from pRS306 & pRSS84
RC   pRSS93 from pRSS84 & pYCF5
RA   Sikorski R.S., Hieter P.;
RT   "A system of shuttle vectors and yeast host strains designed for
RT   efficient manipulation of DNA in Saccharomyces cerevisiae";
RL   Genetics 122:19-27(1989).
XX
RN   [2]
RC   pRS403
RA   Stillman D.J.;
RT   ;
RL   Submitted (10-NOV-1993) by:
RL   Stillman D.J., Dept. of Cellular, Viral and Molecular Biology,
RL   University of Utah Medical Center, Salt Lake City, UT 84132 USA.
XX
CC   Yeast Integrating Plasmid (YIp).
CC   NCBI gi: 416314
CC   NM (pRS403)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Stratagene)
CC   HO (E.coli)(Saccharomyces cerevisiae YPH499 from YNN216/S288C)
CC   HO (Saccharomyces cerevisiae YPH500 from YNN216/S288C)
CC   HO (Saccharomyces cerevisiae YPH501 from YNN216/S288C)
CC   CP ()
CC   FN (cloning)(shuttle)(mutation)(insertion)
CC   SE ()
CC   PA (pBluescript II SK+)
CC   BR (pRS404)(pRS405)(pRS406)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pRS303 4443bp
FT                   linker
FT                   -> pRS403 4456bp"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   reporter gene"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT yeast HIS3 gene"
XX
SQ   Sequence 4456 BP; 1151 A; 1057 C; 1110 G; 1138 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
     gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
     ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
     tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
     atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
     aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
     atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
     cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
     ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
     ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
     ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag
     taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
     atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
     atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
     ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
     aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
     ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
     atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat
     actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt
     ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt
     gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
     ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg
     acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag
     cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
     ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa
     ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     acgaggccct ttcgtc
//