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ID PRS403 preliminary; circular DNA; SYN; 4456 BP.
XX
AC U03443; IG3011;
XX
DT 03-FEB-1994 (Rel. 8, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Saccharomyces/E.coli phagemid vector pRS403 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-4456
RC pRSS56 from pBluescript KS+ & BlueScribe
RC pRS303 from pRSS56 & HIS3 gene
RC pRS304 from pRSS56 & TRP1 gene
RC pRS305 from pRSS56 & LEU2 gene
RC pRS306 from pRSS56 & URA3 gene
RC pUC19-CEN6:32 from pUC19 & CEN6
RC pRSS83 from pUC19-CEN6:32 & pAB9, ARS
RC pRSS84 from pRSS83
RC pRS313 from pRS303 & pRSS84
RC pRS314 from pRS304 & pRSS84
RC pRS315 from pRS305 & pRSS84
RC pRS316 from pRS306 & pRSS84
RC pRSS93 from pRSS84 & pYCF5
RA Sikorski R.S., Hieter P.;
RT "A system of shuttle vectors and yeast host strains designed for
RT efficient manipulation of DNA in Saccharomyces cerevisiae";
RL Genetics 122:19-27(1989).
XX
RN [2]
RC pRS403
RA Stillman D.J.;
RT ;
RL Submitted (10-NOV-1993) by:
RL Stillman D.J., Dept. of Cellular, Viral and Molecular Biology,
RL University of Utah Medical Center, Salt Lake City, UT 84132 USA.
XX
CC Yeast Integrating Plasmid (YIp).
CC NCBI gi: 416314
CC NM (pRS403)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Stratagene)
CC HO (E.coli)(Saccharomyces cerevisiae YPH499 from YNN216/S288C)
CC HO (Saccharomyces cerevisiae YPH500 from YNN216/S288C)
CC HO (Saccharomyces cerevisiae YPH501 from YNN216/S288C)
CC CP ()
CC FN (cloning)(shuttle)(mutation)(insertion)
CC SE ()
CC PA (pBluescript II SK+)
CC BR (pRS404)(pRS405)(pRS406)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pRS303 4443bp
FT linker
FT -> pRS403 4456bp"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
FT promoter 0..0
FT /note="PRO bacteriophage T3"
FT promoter 0..0
FT /note="PRO E. coli lac gene"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ);
FT reporter gene"
FT rep_origin 0..0
FT /note="ORI yeast 2 micron"
FT CDS 0..0
FT /note="ANT yeast HIS3 gene"
XX
SQ Sequence 4456 BP; 1151 A; 1057 C; 1110 G; 1138 T; 0 other;
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag
taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat
actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt
ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt
gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg
acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag
cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa
ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
acgaggccct ttcgtc
//