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ID   PRS416     preliminary; circular DNA; SYN; 4898 BP.
XX
AC   U03450; IG3018;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli phagemid vector pRS416 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4898
RC   pRSS56 from pBluescript KS+ & BlueScribe
RC   pRS303 from pRSS56 & HIS3 gene
RC   pRS304 from pRSS56 & TRP1 gene
RC   pRS305 from pRSS56 & LEU2 gene
RC   pRS306 from pRSS56 & URA3 gene
RC   pUC19-CEN6:32 from pUC19 & CEN6
RC   pRSS83 from pUC19-CEN6:32 & pAB9, ARS
RC   pRSS84 from pRSS83
RC   pRS313 from pRS303 & pRSS84
RC   pRS314 from pRS304 & pRSS84
RC   pRS315 from pRS305 & pRSS84
RC   pRS316 from pRS306 & pRSS84
RC   pRSS93 from pRSS84 & pYCF5
RA   Sikorski R.S., Hieter P.;
RT   "A system of shuttle vectors and yeast host strains designed for
RT   efficient manipulation of DNA in Saccharomyces cerevisiae";
RL   Genetics 122:19-27(1989).
XX
RN   [2]
RC   pRS416
RA   Stillman D.J.;
RT   ;
RL   Submitted (10-NOV-1993) by:
RL   Stillman D.J., Dept. of Cellular, Viral and Molecular Biology,
RL   University of Utah Medical Center, Salt Lake City, UT 84132 USA.
XX
CC   Yeast Centromere Plasmid (YCp).
CC   NCBI gi: 416321
CC   NM (pRS416)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Stratagene)
CC   HO (E.coli)(Saccharomyces cerevisiae YPH499 from YNN216/S288C)
CC   HO (Saccharomyces cerevisiae YPH500 from YNN216/S288C)
CC   HO (Saccharomyces cerevisiae YPH501 from YNN216/S288C)
CC   CP ()
CC   FN (cloning)(shuttle)(mutation)(insertion)
CC   SE ()
CC   PA (pBluescript II SK+)
CC   BR (pRS414)(pRS415)(pRS413)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pRS316 4887bp
FT                   linker
FT                   -> pRS416 4898bp"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   reporter gene"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN; ARS"
XX
SQ   Sequence 4898 BP; 1362 A; 1077 C; 1167 G; 1292 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
     ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
     agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
     cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
     cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
     ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
     aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
     tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
     ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
     aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
     acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
     aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
     gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
     ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata
     agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg
     cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga
     gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt
     gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc
     ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa atatataaat
     taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt
     aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca
     ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt
     gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc
     taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt
     tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa
     aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg
     aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta tcatgacatt
     aacctataaa aataggcgta tcacgaggcc ctttcgtc
//