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ID PRS416 preliminary; circular DNA; SYN; 4898 BP.
XX
AC U03450; IG3018;
XX
DT 03-FEB-1994 (Rel. 8, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Saccharomyces/E.coli phagemid vector pRS416 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-4898
RC pRSS56 from pBluescript KS+ & BlueScribe
RC pRS303 from pRSS56 & HIS3 gene
RC pRS304 from pRSS56 & TRP1 gene
RC pRS305 from pRSS56 & LEU2 gene
RC pRS306 from pRSS56 & URA3 gene
RC pUC19-CEN6:32 from pUC19 & CEN6
RC pRSS83 from pUC19-CEN6:32 & pAB9, ARS
RC pRSS84 from pRSS83
RC pRS313 from pRS303 & pRSS84
RC pRS314 from pRS304 & pRSS84
RC pRS315 from pRS305 & pRSS84
RC pRS316 from pRS306 & pRSS84
RC pRSS93 from pRSS84 & pYCF5
RA Sikorski R.S., Hieter P.;
RT "A system of shuttle vectors and yeast host strains designed for
RT efficient manipulation of DNA in Saccharomyces cerevisiae";
RL Genetics 122:19-27(1989).
XX
RN [2]
RC pRS416
RA Stillman D.J.;
RT ;
RL Submitted (10-NOV-1993) by:
RL Stillman D.J., Dept. of Cellular, Viral and Molecular Biology,
RL University of Utah Medical Center, Salt Lake City, UT 84132 USA.
XX
CC Yeast Centromere Plasmid (YCp).
CC NCBI gi: 416321
CC NM (pRS416)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Stratagene)
CC HO (E.coli)(Saccharomyces cerevisiae YPH499 from YNN216/S288C)
CC HO (Saccharomyces cerevisiae YPH500 from YNN216/S288C)
CC HO (Saccharomyces cerevisiae YPH501 from YNN216/S288C)
CC CP ()
CC FN (cloning)(shuttle)(mutation)(insertion)
CC SE ()
CC PA (pBluescript II SK+)
CC BR (pRS414)(pRS415)(pRS413)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pRS316 4887bp
FT linker
FT -> pRS416 4898bp"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
FT promoter 0..0
FT /note="PRO bacteriophage T3"
FT promoter 0..0
FT /note="PRO E. coli lac gene"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ);
FT reporter gene"
FT rep_origin 0..0
FT /note="ORI yeast 2 micron"
FT CDS 0..0
FT /note="ANT yeast URA3 gene"
FT misc_feature 0..0
FT /note="yeast centromere CEN; ARS"
XX
SQ Sequence 4898 BP; 1362 A; 1077 C; 1167 G; 1292 T; 0 other;
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata
agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg
cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga
gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt
gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc
ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa atatataaat
taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt
aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca
ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt
gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc
taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt
tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa
aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg
aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta tcatgacatt
aacctataaa aataggcgta tcacgaggcc ctttcgtc
//