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ID   PRS426     preliminary; circular DNA; SYN; 5726 BP.
XX
AC   U03451; ATCC77107;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli phagemid vector pRS426 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5726
RC   plasmid from YEp24 & pRSS56
RC   [pBR322/SC4128 from pBR322 & yeast]
RC   pJS92 from plasmid & pBR322/SC4128
RC   pRS323 from pJS92 & pRS303
RC   pRS324 from pJS92 & pRS304
RC   pRS325 from pJS92 & pRS305
RC   pRS326 from pJS92 & pRS306
RC   pRS423 from pRS323 & pBluescript II SK+
RC   pRS424 from pRS324 & pBluescript II SK+
RC   pRS425 from pRS325 & pBluescript II SK+
RC   pRS426 from pRS326 & pBluescript II SK+
RA   Christianson T.W., Sikorski R.S., Dante M., Shero J.H., Hieter P.;
RT   "Multifunctional yeast high-copy-number shuttle vectors";
RL   Gene 110:119-122(1992).
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RN   [2]
RP   1-5726
RC   pRSS56 from pBluescript KS+ & BlueScribe
RC   pRS303 from pRSS56 & HIS3 gene
RC   pRS304 from pRSS56 & TRP1 gene
RC   pRS305 from pRSS56 & LEU2 gene
RC   pRS306 from pRSS56 & URA3 gene
RC   pUC19-CEN6:32 from pUC19 & CEN6
RC   pRSS83 from pUC19-CEN6:32 & pAB9, ARS
RC   pRSS84 from pRSS83
RC   pRS313 from pRS303 & pRSS84
RC   pRS314 from pRS304 & pRSS84
RC   pRS315 from pRS305 & pRSS84
RC   pRS316 from pRS306 & pRSS84
RC   pRSS93 from pRSS84 & pYCF5
RA   Sikorski R.S., Hieter P.;
RT   "A system of shuttle vectors and yeast host strains designed for
RT   efficient manipulation of DNA in Saccharomyces cerevisiae";
RL   Genetics 122:19-27(1989).
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RN   [3]
RC   pRS426
RA   Stillman D.J.;
RT   ;
RL   Submitted (10-NOV-1993) by:
RL   Stillman D.J., Dept. of Cellular, Viral and Molecular Biology,
RL   University of Utah Medical Center, Salt Lake City, UT 84132 USA.
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CC   The order of the major features in this plasmid is:ori(f1) - lacZ - T7
CC   promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR -
CC   ori (2 micron) - URA3.
CC   Contains the REP3 and FRT sequences necessary for high copy
CC   propagation in yeast.
CC   In S. cerevisiae, the copy number is about 20 per haploid cell.  In
CC   non-selective growth, plasmids are lost through mitotic segregation at
CC   rates in the range of 4.4 + or - 1.4% of progeny per doubling.
CC   YE-type shuttle vector encoding the beta-galactosidase alpha peptide,
CC   permitting blue-white visual detection. Can be used to produce ssDNA.
CC   Contains promoters for in vitro RNA synthesis and priming sites useful
CC   for sequencing.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--5.8; EcoRI--5.8; PstI--4.0, 1.7; PvuII--5.2, 0.54. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 416322
CC   NM (pRS426)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli DH5alpha)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR (pRS424)(pRS425)(pRS423)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp24 NdeI-HpaI 1346bp 341..1687,
FT                   \ yeast 2 micron ori [1345bp]
FT                   :Klenow
FT                   2. pRSS56 AatII 2998bp 3140..3140
FT                   T4 DNA polymerase
FT                   -> plasmid 4344bp [unique NdeI]
FT                   1. pBR322 4361bp
FT                   2. yeast 1800bp, TRP1 gene
FT                   -> pBR322/SC4128 6200bp
FT                   1. plasmid NdeI 4344bp 185..185
FT                   Klenow
FT                   2. pBR322/SC4128 BglII-BglII 1800bp, TRP1 gene
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pJS92 6250bp
FT                   1. pJS92 remove DraII-DraII 1800bp, TRP1 gene/4445bp
FT                   2. pRS306 DraII-DraII 1525bp 565..2090, URA3 gene
FT                   -> pRS326 5723bp
FT                   1. pRS326 remove PvuI-PvuI 1913bp 1844..3757,
FT                   \ MCS/3810bp
FT                   2. pBluescript II SK+ PvuI-PvuI 1916bp 501..2417, MCS
FT                   -> pRS426 5726bp"
FT   misc_binding    0..0
FT                   /note="MCS SacI-BstXI-SacII-EagI-NotI-XbaI-SpeI-
FT                   BamHI-SmaI-PstI-
FT                   EcoRI-EcoRV-HindIII-ClaI-SalI-XhoI-ApaI-KpnI"
FT   misc_binding    0..0
FT                   /note="SIT unique SacI-BstXI-SacII-EagI-NotI-SpeI-
FT                   BamHI-SmaI-EcoRI-HindIII-ClaI-SalI-XhoI-KpnI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
XX
SQ   Sequence 5726 BP; 1568 A; 1246 C; 1370 G; 1542 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
     ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
     agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
     cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
     cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
     ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
     aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
     tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
     ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
     aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
     acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
     aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
     gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
     ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata
     agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg
     cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga
     gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt
     gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaacga
     agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
     caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
     cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
     agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa
     aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc
     acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt
     ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac
     ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt
     tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat
     ttcctttgat attggatcat actaagaaac cattattatc atgacattaa cctataaaaa
     taggcgtatc acgaggccct ttcgtc
//