back Return to this vector's summary.
ID   PRSETA     preliminary; circular DNA; SYN; 2827 BP.
XX
AC   IG1111; X54202; X54203; X54204; X54205; X54206; X54207; X54208;
AC   X54209;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pRSETA - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRSETA from pET-3 & pBluescript series
RC   pRSETB from pET-3 & pBluescript series
RC   pRSETC from pET-3 & pBluescript series
RC   pSE280 from pKK233-2
RC   pSE380 from pTrc99A
RC   pSE420 from pSE380
RC   pTRCHISA from pSE420
RC   pTRCHISB from pSE420
RC   pTRCHISC from pSE420
RA   Abdel-Malek H.;
RT   ;
RL   The Digest 4:1-3(1991).
XX
RN   [2]
RC   pAR1151 from pBR322 & phage T7
RC   pAR1219 from pBR322 & phage T7 & lacI/lacUV5
RC   pAR951 from pBR322 & phage T7
RC   pAR1494 from pBR322 & phage T7
RC   pAR946 from pBR322 & phage T7
RC   pAR219 from pBR322 & phage T7
RC   pAR511 from pBR322 & phage T7
RC   pAR1012 from pBR322 & phage T7
RC   pAR525 from pBR322 & phage T7
RC   pAR213 from pBR322 & phage T7
RC   pAR441 from pBR322 & phage T7
RC   pAR436 from pBR322 & phage T7
RA   Studier F.W., Moffatt B.A.;
RT   "Use of bacteriophage T7 RNA polymerase to direct selective
RT   high-level expression of cloned genes";
RL   J. Mol. Biol. 189:113-130(1986).
XX
CC   NM (pRSETA)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pET3)(pBluescript)
CC   BR (pRSETB)(pRSETC)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pET-3
FT                   2. pBluescript series
FT                   -> pRSETA 2827bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1547..1547
FT                   /note="SIT ScaI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1"
FT   terminator      0..0
FT                   /note="TER"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-BstBI-EcoRI-NcoI-KpnI-
FT                   PvuII-PstI-BglII-XhoI-BamHI"
FT   misc_binding    0..0
FT                   /note="SIT enterokinase (EK)"
FT   misc_binding    0..0
FT                   /note="SIT NheI"
FT   repeat_unit     0..0
FT                   /note="RPT histidine (6)"
FT   misc_binding    0..0
FT                   /note="SIT NdeI"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   RBS             0..0
FT                   /note="RBS E. coli g10 gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
XX
SQ   Sequence 2827 BP; 749 A; 664 C; 690 G; 724 T; 0 other;
     catgacctta tgggactttc ctacttggcg tacatctacg tattagtcat cgctattacc
     tggtgatgcg gttttggcag tacatcaatg gcgtggatat gcggggttct catcatcatc
     atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg
     atgacgataa ggatcgatgg ggatccgagc tcgagatctg cagctggtac catggaattc
     gaagcttgat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     tgagcaataa ctagagcggt ttgactcacg gggatttcaa gtctccaccc cattgacgtc
     aatgggagtt gttttggcac caaaatcaac gggactttcc cgaccgctgc gccttatccg
     gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     tcgtgtagat aactacgata cgggagcgct taccatctgg ccccagtgct gcaatgatac
     cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     ccgagcgcag aagtggtcct gcaactttat ccgcctccat tcagtctatt aattgttgcc
     gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt ggcattgcta
     caggcatcgt ggtgtcactc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     tacgggataa tagtgtatca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     gaaaagtgcc acctgtatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     catcaggcga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat atttgttaaa
     tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     agaccgcgat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     catcacccaa atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat cggaacccta
     aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagatcc
     ggatatagtt cctcctttca gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg
     ttatgctagt tattgctcag cggtggcagc agccaactca gcttcctttc gggctttgtt
     agcagccgga tcaagcttcg aattccatgg taccagctgc agatctcgag ctcggatcct
     tatcgtcatc gtcgtacaga tcccgaccca tttgctgtcc accagtcatg ctagccatac
     catgatgatg atgatgatga gaaccccgca tatgtatatc tccttcttaa agttaaacaa
     aattatctag agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga
     tcgagat
//