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ID   PRSETB     preliminary; circular DNA; SYN; 2817 BP.
XX
AC   IG1112; X54202; X54203; X54204; X54205; X54206; X54207; X54208;
AC   X54209;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pRSETB - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRSETA from pET-3 & pBluescript series
RC   pRSETB from pET-3 & pBluescript series
RC   pRSETC from pET-3 & pBluescript series
RC   pSE280 from pKK233-2
RC   pSE380 from pTrc99A
RC   pSE420 from pSE380
RC   pTRCHISA from pSE420
RC   pTRCHISB from pSE420
RC   pTRCHISC from pSE420
RA   Abdel-Malek H.;
RT   ;
RL   The Digest 4:1-3(1991).
XX
CC   NM (pRSETB)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pET3)(pBluescript)
CC   BR (pRSETA)(pRSETC)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pET-3
FT                   2. pBluescript series
FT                   -> pRSETB 2817bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1547..1547
FT                   /note="SIT ScaI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1"
FT   terminator      0..0
FT                   /note="TER"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-BstBI-EcoRI-NcoI-KpnI-
FT                   PvuII-PstI-BglII-XhoI-BamHI"
FT   misc_binding    0..0
FT                   /note="SIT enterokinase (EK)"
FT   misc_binding    0..0
FT                   /note="SIT NheI"
FT   repeat_unit     0..0
FT                   /note="RPT histidine (6)"
FT   misc_binding    0..0
FT                   /note="SIT NdeI"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   RBS             0..0
FT                   /note="RBS E. coli g10 gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
XX
SQ   Sequence 2817 BP; 747 A; 663 C; 685 G; 722 T; 0 other;
     catgacctta tgggactttc ctacttggcg tacatctacg tattagtcat cgctattacc
     tggtgatgcg gttttggcag tacatcaatg gcgtggatat gcggggttct catcatcatc
     atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg
     atgacgataa ggatccgagc tcgagatctg cagctggtac catggaattc gaagcttgat
     ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     ctagagcggt ttgactcacg gggatttcaa gtctccaccc cattgacgtc aatgggagtt
     gttttggcac caaaatcaac gggactttcc cgaccgctgc gccttatccg gtaactatcg
     tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     aactacgata cgggagcgct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     aagtggtcct gcaactttat ccgcctccat tcagtctatt aattgttgcc gggaagctag
     agtaagtagt tcgccagtta atagtttgcg caacgttgtt ggcattgcta caggcatcgt
     ggtgtcactc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     tagtgtatca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     acctgtatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcga
     aattgtaaac gttaatattt tgttaaaatt cgcgttaaat atttgttaaa tcagctcatt
     ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgcgat
     agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa
     atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc
     ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa
     ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagatcc ggatatagtt
     cctcctttca gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagt
     tattgctcag cggtggcagc agccaactca gcttcctttc gggctttgtt agcagccgga
     tcaagcttcg aattccatgg taccagctgc agatctcgag ctcggatcct tatcgtcatc
     gtcgtacaga tcccgaccca tttgctgtcc accagtcatg ctagccatac catgatgatg
     atgatgatga gaaccccgca tatgtatatc tccttcttaa agttaaacaa aattatctag
     agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgagat
//