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ID   PRSETC     preliminary; circular DNA; SYN; 2821 BP.
XX
AC   IG1113; X54202; X54203; X54204; X54205; X54206; X54207; X54208;
AC   X54209;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pRSETC - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRSETA from pET-3 & pBluescript series
RC   pRSETB from pET-3 & pBluescript series
RC   pRSETC from pET-3 & pBluescript series
RC   pSE280 from pKK233-2
RC   pSE380 from pTrc99A
RC   pSE420 from pSE380
RC   pTRCHISA from pSE420
RC   pTRCHISB from pSE420
RC   pTRCHISC from pSE420
RA   Abdel-Malek H.;
RT   ;
RL   The Digest 4:1-3(1991).
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CC   NM (pRSETC)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pET3)(pBluescript)
CC   BR (pRSETA)(pRSETB)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pET-3
FT                   2. pBluescript series
FT                   -> pRSETC 2821bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1547..1547
FT                   /note="SIT ScaI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1"
FT   terminator      0..0
FT                   /note="TER"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-BstBI-EcoRI-NcoI-KpnI-
FT                   PvuII-PstI-BglII-XhoI-BamHI"
FT   misc_binding    0..0
FT                   /note="SIT enterokinase (EK)"
FT   misc_binding    0..0
FT                   /note="SIT NheI"
FT   repeat_unit     0..0
FT                   /note="RPT histidine (6)"
FT   misc_binding    0..0
FT                   /note="SIT NdeI"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   RBS             0..0
FT                   /note="RBS E. coli g10 gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
XX
SQ   Sequence 2821 BP; 748 A; 664 C; 686 G; 723 T; 0 other;
     catgacctta tgggactttc ctacttggcg tacatctacg tattagtcat cgctattacc
     tggtgatgcg gttttggcag tacatcaatg gcgtggatat gcggggttct catcatcatc
     atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg
     atgacgataa ggatcgatgg atccgacctc gagatctgca gctggtacca tggaattcga
     agcttgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     agcaataaag cggtttgact cacggggatt tcaagtctcc accccattga cgtcaatggg
     agttgttttg gcaccaaaat caacgggact ttcccgaccg ctgcgcctta tccggtaact
     atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     agataactac gatacgggag cgcttaccat ctggccccag tgctgcaatg ataccgcgag
     acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     gcagaagtgg tcctgcaact ttatccgcct ccattcagtc tattaattgt tgccgggaag
     ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttggcatt gctacaggca
     tcgtggtgtc actctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     ataatagtgt atcacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     tgccacctgt atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     gcgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatatttgt taaatcagct
     cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     cgatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     ccaaatcaag ttttttgcgg tcgaggtgcc gtaaagctct aaatcggaac cctaaaggga
     gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
     gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag atccggatat
     agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
     tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc
     cggatcaagc ttcgaattcc atggtaccag ctgcagatct cgagctcgga tccttatcgt
     catcgtcgta cagatcccga cccatttgct gtccaccagt catgctagcc ataccatgat
     gatgatgatg atgagaaccc cgcatatgta tatctccttc ttaaagttaa acaaaattat
     ctagagggaa accgttgtgg tctccctata gtgagtcgta ttaatttcgc gggatcgaga
     t
//