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ID   PRSS56     preliminary; circular DNA; SYN; 2998 BP.
XX
AC   IG9907;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli phagemid vector pRSS56 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRSS56 from pBluescript KS+ & pBS+
RC   pRS303 from pRSS56 & HIS3 gene
RC   pRS304 from pRSS56 & TRP1 gene
RC   pRS305 from pRSS56 & LEU2 gene
RC   pRS306 from pRSS56 & URA3 gene
RC   pUC19-CEN6:32 from pUC19 & CEN6
RC   pRSS83 from pUC19-CEN6:32 & pAB9, ARS
RC   pRSS84 from pRSS83
RC   pRS313 from pRS303 & pRSS84
RC   pRS314 from pRS304 & pRSS84
RC   pRS315 from pRS305 & pRSS84
RC   pRS316 from pRS306 & pRSS84
RC   pRSS93 from pRSS84 & pYCF5
RA   Sikorski R.S., Hieter P.;
RT   "A system of shuttle vectors and yeast host strains designed for
RT   efficient manipulation of DNA in Saccharomyces cerevisiae";
RL   Genetics 122:19-27(1989).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pRSS56)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBluescript KS+)(pBS+)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBluescript KS+ PvuI-PvuI 1914bp 498..2412,
FT                   \ MCS
FT                   2. pBS+ PvuI-PvuI 1084bp 2850..3204..730, amp/f1 ori
FT                   -> pRSS56 2998bp [unique NdeI, unique AatII]"
FT   -               1..1914
FT                   /note="pBluescript KS+ 498..2411 1914bp
FT                   PvuI = CGAT^CG"
FT   -               1915..2998
FT                   /note="pBS+ 2850..3204..729 1084bp
FT                   PvuI = CGAT^CG"
FT   misc_binding    0..0
FT                   /note="MCS KpnI-ApaI-XhoI-SalI-ClaI-HindIII-EcoRV-
FT                   EcoRI-PstI-SmaI-
FT                   BamHI-SpeI-XbaI-EagI-NotI-BstXI-SacII-SacI"
FT   misc_binding    0..0
FT                   /note="SIT unique XhoI-SalI-EcoRI-SmaI-BamHI-NotI-
FT                   SacI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2998 BP; 760 A; 746 C; 779 G; 713 T; 0 other;
     ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     gtgaattgta atacgactca ctatagggcg aattggagct ccaccgcggt ggcggccgct
     ctagaactag tggatccccc gggctgcagg aattcgatat caagcttatc gataccgtcg
     acctcgaggg ggggcccggt acccagcttt tgttcccttt agtgagggtt aattccgagc
     ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgctg
     ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaaacgtta
     atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     atgcgccgct acagggcgcg tcgcgccatt cgccattcag gctgcgcaac tgttggga
//