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ID   PRSV5GPT   preliminary; circular DNA; SYN; 4839 BP.
XX
AC   M83236;
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DT   06-JAN-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pRSV.5gpt - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4839
RC   RSV.3-DRalpha from RSV.3 & HLA DRalpha
RC   RSV.3-DR4beta from RSV.3 & HLA DR4beta
RC   RSV.5(gpt) from RSV.3 & pSV2-gpt & pUC12
RC   RSV.5(neo) from RSV.5(gpt) & pSV2-neo
RC   RSV.5(hygro) from RSV.5(gpt) & hygromycin gene
RA   Long E.O., Rosen-Bronson S., Karp D.R., Malnati M., Sekaly R.P.,
RA   Jaraquemada D.;
RT   "Efficient cDNA expression vectors for stable and transient
RT   expression of HLA-DR in transfected fibroblast and lymphoid cells";
RL   Hum. Immunol. 31:229-235(1991).
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CC   NM (pRSV.5gpt)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(human)
CC   CP ()
CC   FN (cloning)(expression cDNA)
CC   SE ()
CC   PA (Rhesus macaque polyomavirus)(Rous sarcoma virus)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. RSV.3 AatII-HindIII 2565bp 991..3556,
FT                   \ [2569bp]
FT                   S1 nuclease:
FT                   2. pSV2-gpt PvuII-BamHI 2244bp, gpt gene
FT                   3. pUC12 HindIII-BamHI 27bp 249..276, MCS
FT                   -> pRSV.5gpt 4839bp"
FT   misc_feature    1..29
FT                   /note="MCS [1]"
FT   CDS             complement(873..1929)
FT                   /note="E. coli guanine-hypoxanthine
FT                   phosphoribosyltransferase gene (gpt); putative [1];
FT                   contains internal stop codons"
FT   rep_origin      2274..4311
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             2274..4311
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp) [1]"
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SQ   Sequence 4839 BP; 1277 A; 1103 C; 1190 G; 1269 T; 0 other;
     cttgggctgc aggtcgactc tagaggatcc agacatgata agatacattg atgagtttgg
     acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     caaatgtggt atggctgatt atgatctcta gtcaaggcac tatacatcaa atattcctta
     ttaacccctt tacaaattaa aaagctaaag gtacacaatt tttgagcata gttattaata
     gcagacactc tatgcctgtg tggagtaaga aaaaacagta tgttatgatt ataactgtta
     tgcctactta taaaggttac agaatatttt tccataattt tcttgtatag cagtgcagct
     ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc tattactaaa cacagcatga
     ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt cttctacctt tctcttcttt
     tttggaggag tagaatgttg agagtcagca gtagcctcat catcactaga tggcatttct
     tctgagcaaa acaggttttc ctcattaaag gcattccacc actgctccca ttcatcagtt
     ccataggttg gaatctaaaa tacacaaaca attagaatca gtagtttaac acattataca
     cttaaaaatt ttatatttac cttagagctt taaatctctg taggtagttt gtccaattat
     gtcacaccac agaagtaagg ttccttcaca aagatccggg gcccactcat aaatccagtt
     gccgccacgg tagccaatca ccgtatcgta taaatcatcg tcggtacgtt cggcatcgct
     catcacaata cgtgcctgga cgtcgaggat ttcgcgtggg tcaatgccgc gccagatcca
     catcagacgg ttaatcatgc gataccagtg agggatggtt ttaccatcaa gggccgactg
     cacaggcggt tgtgcgccgt gattaaagcg gcggactagc gtcgaggttt caggatgttt
     aaagcggggt ttgaacaggg tttcgctcag gtttgcctgt gtcatggatg cagcctccag
     aatacttact ggaaactatt gtaacccgcc tgaagttaaa aagaacaacg cccggcagtg
     ccaggcgttg aaaagattag cgaccggaga ttggcgggac gaatacgacg cccatatccc
     acggctgttc aatccaggta tcttgcggga tatcaacaac atagtcatca accagcggac
     gaccagccgg ttttgcgaag atggtgacaa agtgcgcttt tggatacatt tcacgaatcg
     caaccgcagt accaccggta tccaccaggt catcaataac gatgaagcct tcgccatcgc
     cttctgcgcg tttcagcact ttaagctcgc gctggttgtc gtgatcgtag ctggaaatac
     aaacggtatc gacatgacga atacccagtt cacgcgccag taacgcaccc ggtaccagac
     cgccacggct tacggcaata atgcctttcc attgttcaga aggcatcagt cggcttgcga
     gtttacgtgc atggatctgc aacatgtccc aggtgacgat gtatttttcg ctcatgtgaa
     gtgtcccagc ctgtttatct acggcttaaa aagtgttcga ggggaaaata ggttgcgcga
     gattatagag atctggcgca ctaaaaacca gtatttcaca tgagtccgcg tctttttacg
     cactgcctct ccctgacgcg ggataaagtg gtattctcaa acatatctcg caagcctgtc
     ttgtgtccaa gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
     aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg
     cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca cagaggtggc
     acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     gatgtacggg ccagatatac gcgtatctga ggggactagg gtgtgtttag gcgaaaagcg
     gggcttcggt tgtacgcggt taggagtccc ctcaggatat agtagtttcg cttttgcata
     gggaggggga aatgtagtct tatgcaatac acttgtagtc ttgcaacatg gtaacgatga
     gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     ggtggtacga tcgtgcctta ttaggaaggc aacagacagg tctgacatgg attggacgaa
     ccactgaatt ccgcattgca gagataattg tatttaagtg cctagctcga tacaataaac
     gccatttgac cattcaccac attggtgtgc acctccaag
//