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ID   PRSV5NEO   preliminary; circular DNA; SYN; 5108 BP.
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AC   M83237;
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DT   06-JAN-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pRSV.5neo - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5108
RC   RSV.3-DRalpha from RSV.3 & HLA DRalpha
RC   RSV.3-DR4beta from RSV.3 & HLA DR4beta
RC   RSV.5(gpt) from RSV.3 & pSV2-gpt & pUC12
RC   RSV.5(neo) from RSV.5(gpt) & pSV2-neo
RC   RSV.5(hygro) from RSV.5(gpt) & hygromycin gene
RA   Long E.O., Rosen-Bronson S., Karp D.R., Malnati M., Sekaly R.P.,
RA   Jaraquemada D.;
RT   "Efficient cDNA expression vectors for stable and transient
RT   expression of HLA-DR in transfected fibroblast and lymphoid cells";
RL   Hum. Immunol. 31:229-235(1991).
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CC   NM (pRSV.5neo)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(human)
CC   CP ()
CC   FN (cloning)(expression cDNA)
CC   SE ()
CC   PA (Rhesus macaque polyomavirus)(Rous sarcoma virus)
CC   BR (pRSV3)(pRSV.5gpt)(pRSVneo)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pRSV.5gpt remove HindIII-ApaI 1046bp
FT                   \ 884..1930, gpt/3793bp
FT                   :S1 nuclease
FT                   2. pSV2-neo HindIII 5729bp 3093..3093, Tn5 neo gene
FT                   fill in
FT                   SmaI-HindIII 1319bp 1774..3093, Tn5 neo gene [1322bp]
FT                   -> pRSV.5neo 5108bp"
FT   misc_feature    1..29
FT                   /note="MCS [1]"
FT   CDS             complement(880..2197)
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo),
FT                   from pSV2neo;
FT                   putative; contains internal stop codons [1]"
FT   rep_origin      2543..4580
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322) [1]"
FT   CDS             2543..4580
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp) [1]"
FT   promoter        4581..5108
FT                   /note="PRO Rous sarcoma virus 5' long terminal repeat
FT                   (LTR) [1]"
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SQ   Sequence 5108 BP; 1291 A; 1245 C; 1284 G; 1288 T; 0 other;
     cttgggctgc aggtcgactc tagaggatcc agacatgata agatacattg atgagtttgg
     acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     caaatgtggt atggctgatt atgatctcta gtcaaggcac tatacatcaa atattcctta
     ttaacccctt tacaaattaa aaagctaaag gtacacaatt tttgagcata gttattaata
     gcagacactc tatgcctgtg tggagtaaga aaaaacagta tgttatgatt ataactgtta
     tgcctactta taaaggttac agaatatttt tccataattt tcttgtatag cagtgcagct
     ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc tattactaaa cacagcatga
     ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt cttctacctt tctcttcttt
     tttggaggag tagaatgttg agagtcagca gtagcctcat catcactaga tggcatttct
     tctgagcaaa acaggttttc ctcattaaag gcattccacc actgctccca ttcatcagtt
     ccataggttg gaatctaaaa tacacaaaca attagaatca gtagtttaac acattataca
     cttaaaaatt ttatatttac cttagagctt taaatctctg taggtagttt gtccaattat
     gtcacaccac agaagtaagg ttccttcaca aagatccggg gggtgggcga agaactccag
     catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc
     gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg
     gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa
     gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat
     tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg
     caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg
     acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt
     tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctttttgcaa aagcctaggc
     ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
     ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
     tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact
     aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
     ctaactgaca cacattccac agaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
     aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtatctgag
     gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc
     tcaggatata gtagtttcgc ttttgcatag ggagggggaa atgtagtctt atgcaataca
     cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     acagacaggt ctgacatgga ttggacgaac cactgaattc cgcattgcag agataattgt
     atttaagtgc ctagctcgat acaataaacg ccatttgacc attcaccaca ttggtgtgca
     cctccaag
//