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ID   PRSVNEO    preliminary; circular DNA; SYN; 5736 BP.
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AC   M77786; ATCC37198;
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DT   23-OCT-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pRSVneo - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5736
RC   pRSVneo
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [2]
RC   pRSVgpt from pRSVcat & pSV2-gpt
RC   pRSVneo from pRSVcat & pSV2-neo
RC   pRSV-beta-globin from pRSVcat & pSV2-beta-G
RA   Gorman C.M., Padmanabhan R., Howard B.H.;
RT   "High efficiency DNA-mediated transformation of primate cells";
RL   Science 221:551-553(1983).
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RN   [3]
RC   pSV2 from pBR322 & SV40
RC   pSV2-cat from pSV2
RC   pRSVneo, pRSVcat, pRSVgpt, pRSV/betaglobin from RSV
RA   Gorman C.M.;
RT   "High efficiency transfer into mammalian cells";
RL   DNA Cloning 2:143-190(1985).
RL   Glover D.M., ed., IRL Press, Oxford.
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CC   Escherichia coli/mammalian cell culture vector with selectable
CC   markers in both systems. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pRSVneo)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)(human)(vertebrate cells)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Rous sarcoma virus)(Rhesus macaque polyomavirus)(Tn5)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. RSV, LTR
FT                   3. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   4. SV40, small t intron/early polyA
FT                   -> pRSVcat
FT                   1. pRSVcat remove BamHI-HindIII, cat gene/SV40/2348bp
FT                   2. pSV2-neo BamHI-HindIII 2341bp 752..3093,
FT                   \ neo gene/SV40/3388bp
FT                   -> pRSVneo 5736bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   promoter        0..0
FT                   /note="PRO Rous sarcoma virus (RSV)
FT                   long terminal repeat (LTR)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo)"
FT                   kanamycin resistance gene (kan)"
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SQ   Sequence 5736 BP; 1488 A; 1438 C; 1353 G; 1457 T; 0 other;
     cttggaggtg cacaccaatg tggtgaatgg tcaaatggcg tttattgtat cgagctaggc
     acttaaatac aattatctct gcaatgcgga attcagtggt tcgtccaatc catgtcagac
     ctgtctgttg ccttcctaat aaggcacgat cgtaccacct tacttccacc aatcggcatg
     cacggtgctt tttctctcct tgtaaggcat gttgctaact catcgttacc atgttgcaag
     actacaagtg tattgcataa gactacattt ccccctccct atgcaaaagc gaaactacta
     tatcctgagg ggactcctaa ccgcgtacaa ccgaagcccc gcttttcgcc taaacacacc
     ctagtcccct cagatacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct
     aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat
     tttgctcgcg cactactcag cgacctccaa cacacaagca gggagcagat actggcttaa
     ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     agaattcctt tgcctaattt aaatgaggac ttaacctgtg gaaatatttt gatgtgggaa
     gctgttactg ttaaaactga ggttattggg gtaactgcta tgttaaactt gcattcaggg
     acacaaaaaa ctcatgaaaa tggtgctgga aaacccattc aagggtcaaa ttttcatttt
     tttgctgttg gtggggaacc tttggagctg cagggtgtgt tagcaaacta caggaccaaa
     tatcctgctc aaactgtaac cccaaaaaat gctacagttg acagtcagca gatgaacact
     gaccacaagg ctgttttgga taaggataat gcttatccag tggagtgctg ggttcctgat
     ccaagtaaaa atgaaaacac tagatatttt ggaacctaca caggtgggga aaatgtgcct
     cctgttttgc acattactaa cacagcaacc acagtgcttc ttgatgagca gggtgttggg
     cccttgtgca aagctgacag cttgtatgtt tctgctgttg acatttgtgg gctgtttacc
     aacacttctg gaacacagca gtggaaggga cttcccagat attttaaaat tacccttaga
     aagcggtctg tgaaaaaccc ctacccaatt tcctttttgt taagtgacct aattaacagg
     aggacacaga gggtggatgg gcagcctatg attggaatgt cctctcaagt agaggaggtt
     agggtttatg aggacacaga ggagcttcct ggggatccag acatgataag atacattgat
     gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     aacctctaca aatgtggtat ggctgattat gatctctagt caaggcacta tacatcaaat
     attccttatt aaccccttta caaattaaaa agctaaaggt acacaatttt tgagcatagt
     tattaatagc agacactcta tgcctgtgtg gagtaagaaa aaacagtatg ttatgattat
     aactgttatg cctacttata aaggttacag aatatttttc cataattttc ttgtatagca
     gtgcagcttt ttcctttgtg gtgtaaatag caaagcaagc aagagttcta ttactaaaca
     cagcatgact caaaaaactt agcaattctg aaggaaagtc cttggggtct tctacctttc
     tcttcttttt tggaggagta gaatgttgag agtcagcagt agcctcatca tcactagatg
     gcatttcttc tgagcaaaac aggttttcct cattaaaggc attccaccac tgctcccatt
     catcagttcc ataggttgga atctaaaata cacaaacaat tagaatcagt agtttaacac
     attatacact taaaaatttt atatttacct tagagcttta aatctctgta ggtagtttgt
     ccaattatgt cacaccacag aagtaaggtt ccttcacaaa gatccgggac caaagcggcc
     atcgtgcctc cccactcctg cagttcgggg gcatggatgc gcggatagcc gctgctggtt
     tcctggatgc cgacggattt gcactgccgg tagaactccg cgaggtcgtc cagcctcagg
     cagcagctga accaactcgc gaggggatcg agcccggggt gggcgaagaa ctccagcatg
     agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac
     ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg
     tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct
     tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac
     cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     ctgtctcttg atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca
     tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg
     gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag
     ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat
     tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc
     agcccttgcg ccctgagtgc ttgcggcagc gtgaag
//