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ID   PRX1       preliminary; circular DNA; SYN; 2851 BP.
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AC   M28835; M28836; M28837; ATCC37677;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pRX-1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRX-1, pRX-2, pRX-3 from pATH1 & linkers
RA   Rimm D.L., Pollard T.D.;
RT   "New plasmid vectors for high level synthesis of eukaryotic fusion
RT   proteins in Escherichia coli";
RL   Gene 75:323-327(1989).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--2.85; ClaI--2.85. (ATCC staff)
CC   When E.coli CAG-456 (a protease deficient host) is used this
CC   vector yields large amounts (100 mg/liter or 45% of the soluble
CC   protein) of the fusion protein.
CC   One of a series of plasmid expression vectors (ATCC 37677 to 37679)
CC   representing multiple reading frames for expression from trpE
CC   promoter.
CC   The fusion proteins produced contain less than 25 amino acids of the
CC   trpE protein.
CC   This vector contains the trpE operon and 51 bp of the trpE gene,
CC   followed by an 8-mer EcoRI linker and the M13mp13 multiple cloning
CC   site (MCS).
CC   There is a TGA stop codon (in frame from the GAA of the EcoRI site)
CC   33 bp beyond the MCS ClaI site.
CC   Use E.coli SG935 (ATCC 39623), SG936 (ATCC 39624) or WM6 (ATCC
CC   47020) as host for high level production of recombinant fusion
CC   protein. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pRX-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli CAG-456)(E.coli DH1)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pATH1)(M13mp13)
CC   BR (pRX-2)(pRX-3)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pATH1 NruI 3779bp 476..476,
FT                   \ 51 bp 3' to trpE init
FT                   2. EcoRI linker 8bp cgaattcg
FT                   -> plasmid 3787bp
FT                   1. plasmid remove EcoRI-EcoRI 930bp 476..1406,
FT                   \ trpE gene/2857bp
FT                   -> pRX-1 2876bp"
FT   -               1..475
FT                   /note="pATH1 1..475 475bp
FT                   NruI = TCG^CGA
FT                   \          cg"
FT   -               476..477
FT                   /note="cg 2bp
FT                   \      cg
FT                   EcoRI = G^AATTC"
FT   -               478..2851
FT                   /note="pATH1 1406..3779 2374bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacI-SmaI-BamHI-XbaI-SalI-HindIII-
FT                   ClaI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SacI-SmaI-BamHI-XbaI-SalI-
FT                   HindIII-ClaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli trpE gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 2851 BP; 718 A; 696 C; 716 G; 721 T; 0 other;
     cagctgtggt gtcatggtcg gtgatcgcta gggtgccgag cgcatctcga ctgcacggtg
     caccaatgct tctggcgtca ggtagttatt ggaaagctgt ggtatggctg tgcaggtcgt
     aaatcactgc ataactcgct gctgcctaag gcgcactccc gttctggata atgttttttg
     cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc
     gaactagtta actagtacgc aagttcacgt aaaaagggta tcgacaatga aagcaatttt
     cgtactgaaa ggttggtggc gcacttcctg aaacgggcag tgtattcacc atgcgtaaag
     caatcagata cccagcccgc ctaatgagcg ggcttttttt tgaacaaaat tagagaataa
     caatgcaaac acaaaaaccg actctcgaac tgctaacctg cgaaggcgct tatcgcgaat
     tcgagctcgc ccggggatcc tctagagtcg acctgcagcc caagcttatc gatgataagc
     tgtcaaacat gagaattaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
     caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     gttttcaccg tcatcaccga aacgcgcgag g
//