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ID   PS194      preliminary; circular DNA; PRO; 4397 BP.
AC   X06627; ATCC37082;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Bacillus/Staphylococcus plasmid vector pS194 - complete.
KW   cloning vector.
OS   Staphylococcus aureus
OC   Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
OC   Micrococcaceae.
RN   [1]
RP   1-4397
RC   pS194
RA   Projan S.J., Moghazeh S., Novick R.P.;
RT   ;
RL   Submitted (26-JAN-1988) on tape to the EMBL Data Library by:
RL   Projan S.J., Moghazeh S., Novick R.P.
RN   [2]
RP   1-4397
RC   pS194 from Staphylococcus aureus, strep gene
RC   pRN5202 from pE5
RC   pRN5170 from pRN5202 & pS194, palA
RC   pRN5169 from pRN5202 & pUB110
RC   pS194, pS169, pS177, pUB109 from Staphylococcus inc5 plasmids, strep
RC   pT181, pC221, pS194, pS169 from Staphylococcus inc5 plasmids, strep
RC   pS177, pUB109 from Staphylococcus inc5 plasmids, strep
RA   Projan S.J., Moghazeh S., Novick R.P.;
RT   "Nucleotide sequence of pS194, a streptomycin-resistance plasmid
RT   from Staphylococcus aureus";
RL   Nucleic Acids Res. 16:2179-2187(1988).
RN   [3]
RC   pT181, pC221, pUB112, pC223, pS194, pCW7 from Staph tet plasmids
RC   pT181 from oriC
RC   pC221 from oriD
RC   pUB112 from oriI
RC   pC223 from oriJ
RC   pS194 from oriE1
RC   pCW7 from oriE2
RC   pRN series from pRN5101 & Staph tet plasmids
RC   pWN series from pBluescript series & Staph tet plasmids
RA   Wang P.Z., Projan S.J., Henriquez V., Novick R.P.;
RT   "Origin recognition specificity in pT181 plasmids is determined by
RT   a functional asymmetric palindromic DNA element";
RL   EMBO J. 12:45-52(1993).
RN   [4]
RC   pC194 from S.aureus
RC   pS194 from S.aureus
RC   pSC194 from pS194 & pC194
RA   Lofdahl S., Sjostrom J.E., Philipson L.;
RT   "Characterization of small plasmids from Staphylococcus aureus";
RL   Gene 3:149-159(1978).
RN   [5]
RC   pRN6428 from pRN5101 & pS194
RC   pRN6647 from pRN5101 & pC223
RC   pRN6648 from pRN5101 & pUB112
RC   pRN5601 from pS194
RC   pRN5532 from pC223
RC   pRN5534 from pUB112
RA   Projan S.J., Novick R.P.;
RT   "Comparative analysis of five related Staphylococcal plasmids";
RL   Plasmid 19:203-221(1988).
RN   [6]
RC   pSA1212 from pE5 & linker
RC   pSA7500 from pRN8237 & pSA1212
RC   pSA7540 from pSA7500 & oligo
RC   pSA7542 from pSA7540
RC   pSA7545 from pSA7540 & pRN8061
RC   pSA7550 from pSA7540 & pSA4000
RC   pSA7560 from pSA7550 & pSA7540
RC   pSA7461 from pSA7560
RC   pSA7612 from pSA7600 & pT181
RC   pSA7631 from pSA7600 & pC221
RC   pSA7677 from pSA7600 & pT181
RC   pSA7690 from pSA7650 & pT181
RC   pSA7699 from pSA7650 & pC221
RA   Iordanescu S.;
RT   "Specificity of the interactions between the Rep proteins and the
RT   origins of replication of Staphylococcus aureus plasmids pT181
RT   and pC221";
RL   Mol. Gen. Genet. 217:481-487(1989).
RN   [7]
RC   pCW2 from Staphylococcus aureus JB48126, tet gene
RC   pCW3 from Staphylococcus aureus JB48827, tet gene
RC   pCW4 from Staphylococcus aureus JB48125, tet gene
RC   pCW5 from Staphylococcus aureus JB38, tet gene
RC   pCW6 from Staphylococcus aureus JB48126, cat gene
RC   pCW7 from Staphylococcus aureus JB48827, cat gene
RC   pCW8 from Staphylococcus aureus JB96, cat gene
RC   pCW12 from pC221 & pI258, ery gene
RC   pCW13 from pC221 & pI258, ery gene
RC   pCW14 from pCW8 & pI258, ery gene
RC   pCW17 from pCW13 & phage psi11
RC   pCW18 from pCW14 & phage psi11
RC   pCW19 from pCW14 & phage psi11
RC   pCW20 from pCW14 & phage psi11
RA   Wilson C.R., Baldwin J.N.;
RT   "Characterization and construction of molecular cloning vehicles
RT   within Staphylococcus aureus";
RL   J. Bacteriol. 136:402-413(1978).
RN   [8]
RC   pT181 from Staphylococcus, tet gene
RC   pSA0301 from pT181
RA   Iordanescu S.;
RT   "Temperature-sensitive mutant of a tetracycline resistance
RT   staphylococcal plasmid";
RL   Arch. Roum. Pathol. Exp. Microbiol. 35:257-264(1976).
RN   [9]
RC   pCW7 from Staphylococcus
RC   pCW7, pC221, pC223, pUB112, pS194, pT181 from Staph tet plasmids
RA   Balson D.F., Shaw W.V.;
RT   "Nucleotide sequence of the rep gene of staphylococcal plasmid
RT   pCW7";
RL   Plasmid 24:74-80(1990).
RN   [10]
RC   pT181 from Staphylococcus aureus
RA   Iordanescu S.;
RT   "Three distinct plasmids originating in the same Staphylococcus
RT   aureus strain";
RL   Arch. Roum. Pathol. Exp. Microbiol. 35:111-118(1976).
RN   [11]
RC   pS194 from S.aureus
RC   pT181 from S.aureus, tet gene
RC   pT169 from S.aureus, tet gene
RC   pP169 from S.aureus, pen gene
RC   pI181 from S.aureus
RC   pPI258 from S.aureus, pen gene
RC   pPI524 from S.aureus, pen gene
RC   pPII147 from S.aureus, pen gene
RC   pC221 from S.aureus, cat gene
RC   pC223 from Staphylococcus, cat gene
RC   pK545 from Staphylococcus, kan gene
RA   Novick R.P., Bouandchaud D.;
RT   "Extrachromosomal nature of drug resistance in Staphylococcus
RT   aureus";
RL   Ann. N.Y. Acad. Sci. 182:279-294(1971).
RN   [12]
RC   pSH2 from S.aureus kan gene
RC   from pSH1 & pC22.1
RA   Stiffler P.W., Sweeney H.M., Cohen S.;
RT   "Co-transduction of plasmid mediated resistance to tetracycline
RT   and chloramphenicol in Staphylococcus aureus";
RL   J. Bacteriol. 120:934-944(1974).
RN   [13]
RC   pC194, pS194 from Staphylococcus
RC   pSA0501 or pS194 seg-I from pS194
RA   Iordanescu S., Greceanu V.;
RT   "Localisation du determinant de la streptomycino-resistance chez
RT   une souche de Staphylococcus aureus";
RL   Arch. Roum. Pathol. Exp. Microbiol. 30:463-471(1971).
RN   [14]
RC   pIM13 from B.subtilis
RC   pE5 or pE12 from S.aureus
RC   pBD347 from pC194 & pIM13
RC   pBD367 from pE5
RC   pBD373 from pBD347 & pE5
RC   pROJ5201 from pE5
RA   Projan S.J., Monod M., Narayanan C.S., Dubnau D.;
RT   "Replication properties of pIM13, a naturally occurring plasmid
RT   found in Bacillus subtilis, and of its close relative pE5, a
RT   plasmid native to Staphylococcus aureus";
RL   J. Bacteriol. 169:5131-5139(1987).
RN   [15]
RC   pRN3208 from pI258
RC   p913 from S.aureus, kan gene
RC   p661 from S.aureus, kan gene
RA   Archer G.L., Johnston J.L.;
RT   "Self-transmissible plasmids in staphylococci that encode
RT   resistance to aminoglycosides";
RL   Antimicrob. Agents Chemother. 24:70-77(1983).
RN   [16]
RC   pNE131 from S.epidermidis; [similar to pIM13/pE5]
RA   Lampson B.C., Parisi J.T.;
RT   "Nucleotide sequence of the constitutive
RT   macrolide-lincosamide-streptogramin B resistance plasmid pNE131
RT   from Staphylococcus epidermis and homolopgies with Staphylococcus
RT   aureus plasmids pE194 and pSN2";
RL   J. Bacteriol. 167:888-892(1986).
RN   [17]
RC   pE5 or pE12 from S.aureus
RA   Keller G., Schliefer K.H., Gotz F.;
RT   "Construction and characterization of plasmid vectors for cloning
RT   in Staphylococcus aureus and Staphylococcus carnosus";
RL   Plasmid 10:279-289(1983).
RN   [18]
RC   pE5 or pE12 from S.aureus
RC   pIM13 from Bacillus subtilis
RC   pBD347 from pC194 & pIM13
RA   Monod M., Denoya C., Dubnau D.;
RT   "Sequence and properties of pIM13, a
RT   macrolide-lincosamide-streptogramin B resistance plasmid from
RT   Bacillus subtilis";
RL   J. Bacteriol. 167:138-147(1986).
RN   [19]
RC   pSA5040 from pSA5000 & linker
RA   Iordanescu S.;
RT   "The Inc3B determinant of plasmid pT181. A mutational analysis";
RL   Mol. Gen. Genet. 207:60-67(1987).
RN   [20]
RC   pBD364 from B.sphaericus ery gene
RA   Monod M., Dubnau D.;
RT   "Cloning and analysis of ermG, a new
RT   macrolide-lincosamide-streptogramin B resistance element from
RT   Bacillus sphaericus";
RL   J. Bacteriol. 169:340-350(1987).
RN   [21]
RC   pC22.1 from S.aureus
RA   Shaw W.V., Brodsky R.F.;
RT   "Characterization of chloramphenicol acetyltransferase from
RT   chloramphenicol-resistant Staphylococcus aureus";
RL   J. Bacteriol. 95:28-36(1968).
RN   [22]
RC   pSH1 from S.aureus
RA   Sweeney H.M., Cohen S.;
RT   "Wild-type strain of Staphylococcus aureus containing two genetic
RT   linkage groups for penicilinase production";
RL   J. Bacteriol. 96:920-924(1968).
RN   [23]
RC   pNE131 from S.epidermidis; [similar to pIM13]
RA   Parisi J.T., Robbins J., Lampson B.C., Hecht D.W.;
RT   "Characterization of a macrolide, lincosamide, streptogramin
RT   resistance plasmid in Staphylococcus epidermidis";
RL   J. Bacteriol. 148:559-564(1981).
RN   [24]
RC   pSH2 from S.aureus kan gene
RA   Stiffler P.W., Sweeney H.M., Schneider M., Cohen S.;
RT   "Isolation and characterization of a kanamycin resistance plasmid
RT   from Staphylococcus aureus";
RL   Antimicrob. Agents Chemother. 6:516-520(1974).
CC   Vector for construction and propagation of hybrid DNA in
CC   Staphylococcus aureus or Bacillus subtilis.
CC   Contains one EcoRI and one HindIII site and no sites for BamHI or
CC   SmaI.
CC   Medium is 1065 LB medium.
CC   NM (pS194)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Staphylococcus aureus)(Bacillus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. Staphylococcus aureus
FT                   -> pS194 4397bp [unique HindIII, EcoRI]"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   rep_origin      0..0
FT                   /note="ORI Staphylococcus aureus"
FT   CDS             join(4323..4397,1..771)
FT                   /note="ANT E. coli streptomycin resistance gene (str)
FT                   ORF"
FT   terminator      835..861
FT                   /note="TER palindrome (t1)"
FT   CDS             1424..2386
FT                   /note="GEN E. coli rlx gene ORF"
FT   CDS             2121..2810
FT                   /note="GEN E. coli orfD"
FT   terminator      2826..2983
FT                   /note="TER palindrome (palA)"
FT   CDS             3371..4315
FT                   /note="GEN E. coli repE gene ORF"
SQ   Sequence 4397 BP; 1690 A; 526 C; 861 G; 1320 T; 0 other;
     ctcgagggtt caagaactaa tgaaaatata aaaaaagata agtttcaaga ttatgatttt
     gcttttttcg tatcagatat tgagtatttc acacatgaag aaagttggtt aagtttattt
     ggagaattat tgtttataca gaaaccagaa gatatggaat tattcccacc tgatttagat
     tatggttaca gttatataat gtattttaaa gatggcataa aaatggatat tacattaatt
     aatttaaaag atttaaatcg ttattttagt gattctgatg gtcttgtaaa aattttagtt
     gataaagata atttagtaac tcaagaaatt gttccagatg actcaaatta ttggttaaaa
     aaaccaacag aacgagaatt ttatgattgc tgtaatgagt tttggagtgt ctcaacgtat
     gtagcaaagg gtgtttttag aagagaaata ttatttgctt tagatcattt caataatatt
     ttacgtcctg aattattaag aatgatttct tggtatattg gctttaatag gggttttgat
     tttagtttag gaaagaatta taagtttata aacaaatatt taactgataa agaattcaat
     atgcttttag ctacttttga gatgaatgga tatagaaaga cataccaatc ttttaagctt
     tgttgtgaat tatttaaata ttattcaaat aaagtaagtt gtttaggaaa ttataactat
     ccaaattacg aaaaaaatat tgagaatttt attcgtaata attatgagaa ttaattttat
     tagcaaccac atttttggtt gcttttgttt tgattttgaa tttggttttg aactatgagt
     ggctagcatt tttccactca ttttttgcgt tagcaaaaac ataaagggtc tgggattaat
     cccagcaagc cggtatattc agaacgaagt ggctagaata tacgacgctt gccaaaccac
     tatattactt gagaaaatat agattatatt ataagctata tttatgaaaa ggaatatttt
     tcgtcagaaa ggtggtggga cttgtgaacg aacataaaga taatttggct agcgatttgt
     ctgttggggg aaaccgtaaa cccaaccgca aagagccaaa acaaattagt ttcagagtga
     gtgaacagga atattcaaag ttgaaacagt cggcagaaac tttgaatatg acagtgccta
     atttcgttaa gaaaaaggca caaggcagtc gattagtgtc gccaaaatta gacaaagaaa
     cgcgacaatc gattgccaaa gatttaagtc atttgggtag taatgtgaat cagattgcta
     agtggtttaa tcaaaataaa gataaggcta cacatttacc agaagagaaa tatcatgatt
     tagaaaagca atttgatgat gtgaaaaagg agttgcataa gatatggcaa caactaaatt
     aggcaatacg aagtcggcaa gtcgtgccat taattacgca gaagaacgtg cagaagaaaa
     aagcggctta aattgtgatg ttgattatgc taaatcatat tttaagcaaa ctagggcgtt
     atatggcaaa gagaatggtg ttcaagcaca tacagtcatt caatctttta agcctggcga
     agtaacagct aaagagtgta atgagatagg tttagaatta gctaaaaaaa ttgcaccaga
     ttatcaagtt gcagtttata cacatacaga caaagaccat tatcacaatc acattattat
     taattcagta aatttagaaa caggcaacaa gtatcagtca aacaaagaac aacgtgattt
     tataaaaaag gcgaatgatc agttatgtga agaacgtggt ttatctgtgc cagaaaagtc
     atcagaaatc agatatacgt tggcagaaca gaacatgata gataaagata aaaggtcatg
     gaaaaatgat ataagaatgg cagttgaaga aacaaaggat aacgcagtag cgtttgaaga
     atttaatact ttgctaaaag aaaaaggtgt tgaaatcacg agagtaacga aaaacaacgt
     aacgtataga cacattgaag aagataaaaa agtacgtggg aataagttgg gcgattctta
     tgacaaagga gtgattgaaa atggctttgc aatcgagaaa ttcagacgag aacgagaaga
     agaacgagaa tacgatgaat atgcagacac gttcgaagtt gactgggacg cattcgcaga
     aaattcagaa gaccttagaa aacgaagaat tgcaagaact gaagaaacaa aacaagctag
     taataaaata tatatcagag atgaaagaac aacaggactt gagagaaaag gaattgcagg
     caatcaagtc gagtttgaaa aagacgacgg aggactttca aggtagaggt aaaaaagtcc
     ataatgattt tgttcgtttg ttacaaaaga atttaaatcg agtgaatgca gaagatattg
     agatagaggt tcgaaaagat gtttatggag ttcgagaaga aattcattcg atgttgcgtg
     aagttaaaca atcacatgaa cattatcaaa aaagacaaaa gcaattattt actggtattg
     gtgcaatgtt attagtgttt atgctttttg ctttgattat gacgattggc agagatttta
     tgggcttttt acatgtagat gtattacaga atgccatagc aagcaaaata aaggcttctg
     agggctttat ttcagtttta tggtacattg cttatggttt accttatata ctcgctatag
     ggctatttat ttggttgtat gagtggataa gagcaagatt ccatgattaa acaaagtatg
     atcaggcttt tatgccgaga aaatttattt atattgagaa gtacccttaa ctaaacttgc
     agacgaatgt cgcatagcgt gagtattagc ccgaccattc gacaagtttt gggtttgtta
     agggtacaga ggctcaatgt aaataaagca attggaataa agcaacaaag ggggttgaag
     aaaatgagta agaagaaaaa gattaattta gcaatatcaa ttgctcaagt agtagtgcag
     gcgttaatac tttataacac ttatcagcaa tataaatatt ctaaaaaata ttcagcctaa
     taaaaaaaga cttagtctga atagaccaag ccttttgtta gtgttatatt aatatgcaaa
     taaaaaaaca aaaaaagaag tcgctcactc cctgaccaaa gtttgtgaat gcgacttacc
     ttaaagaaaa acagttggtg tttttttctt aatttttgta tatctagata ttaaactgat
     attggtttat tcttcaagat atatattcgg gtgagcgact tctttaaaca aaataaggag
     tcgatttttt atgagtaaaa aagcagaaga aattcaggca aaacaaagtt tggaaaagga
     aaactcaaat ttttctaaaa ccggatactc taatagccgg ttaaaccgac atattatgta
     cacccccgaa ccaaaattac actttgatgc tatgacaatt gttgggaatc ttaataagaa
     caatgctcac aaactgtctg aatttatgag tattgcgcca caaattagac tttgggatat
     attacaaacc aaatttaaag ctaaggctct acaagaaaaa gtttacatcg aatatgataa
     agtaaaagca gatgcgtggg atagacgtaa tatgcgtgtt gaatttaatc ctaataaact
     tacgcatgaa gaaatgcttt ggttaaaaca aaacatcatt gactacatgg aagatgatgg
     ttttacaaga ttagatttgg cttttgattt tgaagacgat ttgagcgatt attatgcgat
     gactgataaa tcagttaaga aaactatttt ttatggtcgt aacggtaaac cagaaacaaa
     atattttggt gttcgtgaca gtgatagatt tattagaatt tataataaaa aacaagaacg
     taaggataat gcagacattg aagttatgtc tgaacactta tggcgtgtag aaattgaatt
     aaaaagagat atggttgatt attggaacga ttgttttaat gatttacata ttttgaaacc
     agattggtct agtttagaaa aagtaaaaga ccaagcaatg atttatatgc taattcatga
     agaaagtaca tggggtaaat tagaaagacg tacaaaaaat aaatacagag aaatgttaaa
     aagtatttca gaaattgatt taacggactt aatgaaattg actttaaaag agaatgaaaa
     acaattgcaa aagcagattg aattttggca gcgtgaattt agattttggg agtgaaaaaa
     caatgaggac tgaaaaagaa attttaaatt tagtttctga atttgcttat caacgaagca
     atgtaaaaat tattgct