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ID   PSBC1      preliminary; circular DNA; SYN; 3487 BP.
XX
AC   X68257;
XX
DT   24-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mammal bicistronic transcription unit pSBC-1 - complete.
XX
KW   cloning vector.
XX
OS   None
OC   Artificial sequences.
XX
RN   [1]
RP   1-3487
RC   pSBC-1 from pUC8
RC   pSBC-2 from pUC8
RA   Dirks W.G., Wirth M., Hauser H.;
RT   "Dicistronic transcription units for gene expression in mammalian
RT   cells";
RL   Gene 128:247-249(1993).
XX
RN   [2]
RP   1-3487
RC   pSBC-1
RA   Dirks W.G.;
RT   ;
RL   Submitted (27-AUG-1992) on tape to the EMBL Data Library by:
RL   W.G. Dirks, Gesellschaft f/r Biotechnologische Forschung mbH (GBF),
RL   Mascheroder Weg 1, D-3300 Braunschweig, FRG.
XX
CC   Related sequences:Ardelt et al., Gene. 68: 213-219 (88)
CC                     Sarnow., J. Virol. 63: 467-470 (89)
CC   NCBI gi: 58272
CC   NM (pSBC-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC8)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 2665bp
FT                   2. SV40, promoter/enhancer
FT                   3. poliovirus, 19S gene/polyA
FT                   -> pSBC-1 3487bp"
FT   promoter        complement(5..353)
FT                   /note="from SV40"
FT   enhancer        complement(5..353)
FT                   /note="from SV40"
FT   mRNA            complement(363..421)
FT                   /note="RNA poliovirus type 1 19S"
FT   misc_feature    complement(425..472)
FT                   /note="MCS pUC8 polylinker"
FT   5'UTR           complement(473..1096)
FT                   /note="poliovirus type 1 cDNA"
FT   polyA_signal    complement(1121..1357)
FT                   /note="PLA poliovirus type 1 (VP1)"
XX
SQ   Sequence 3487 BP; 869 A; 872 C; 846 G; 900 T; 0 other;
     agctagcttc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccctcga
     ggaactggaa aaccagaaag ttaactggta agtttagtct ttttgtcttt tatttcaggt
     cccggaattc gagctcgccc ggggatcctc tagagtcgac ctgcagaagc ttttaaaaca
     gctctggggt tgtacccacc ccagaggccc acgtggcggc tagtactccg gtattgcggt
     acccttgtac gcctgtttta tactcccttc ccgtaactta gacgcacaaa accaagttca
     atagaagggg gtacaaacca gtaccaccac gaacaagcac ttctgtttcc ccggtgatgt
     cgtatagact gcttgcgtgg ttgaaagcga cggatccgtt atccgcttat gtacttcgag
     aagcccagta ccacctcgga atcttcgatg cgttgcgctc agcactcaac cccagagtgt
     agcttaggct gatgagtctg gacatccctc accggtgacg gtggtccagg ctgcgttggc
     ggcctaccta tggctaacgc catgggacgc tagttgtgaa caaggtgtga agagcctatt
     gagctacata agaatcctcc ggcccctgaa tgcggctaat cccaacctcg gagcaggtgg
     tcacaaacca gtgattggcc tgtcgtaacg cgcaagtccg tggcggaacc gactactttg
     ggtgtccgtg tttcctttta ttttattgtg gctgcttatg gtgacaatca cagattgtta
     tcataaagcg aattggattg cggccgctta agctagcttc gatccagaca tgataagata
     cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     caagtaaaac ctctacaaat gtggtatggc tgattatgat cctgcctcgc gcgtttcggt
     gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     catcagagca gattgtactg agagtgcacc atatgtcggg ccgcgttgct ggcgtttttc
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     agagtaagta gttcgccagt taatagtgcg caacgttgtt gccattgcta caggcatcgt
     ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     gaggcccttt cgtcttcaag aattggtcga tcgaccaatt ctcatgtttg acagcttatc
     atcgata
//