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ID   PSBC2      preliminary; circular DNA; SYN; 2860 BP.
XX
AC   X68258;
XX
DT   24-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mammal bicistronic transcription unit pSBC-2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-3487
RC   pSBC-1 from pUC8
RC   pSBC-2 from pUC8
RA   Dirks W.G., Wirth M., Hauser H.;
RT   "Dicistronic transcription units for gene expression in mammalian
RT   cells";
RL   Gene 128:247-249(1993).
XX
RN   [2]
RP   1-3487
RC   pSBC-2
RA   Dirks W.G.;
RT   ;
RL   Submitted (27-AUG-1992) on tape to the EMBL Data Library by:
RL   W.G. Dirks, Gesellschaft f/r Biotechnologische Forschung mbH (GBF),
RL   Mascheroder Weg 1, D-3300 Braunschweig, FRG.
XX
CC   Related sequences:Ardelt et al., Gene. 68: 213-219 (88)
CC                     Sarnow., J. Virol. 63: 467-470 (89)
CC   NM (pSBC-2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC8)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 2665bp
FT                   2. SV40, promoter/enhancer
FT                   3. poliovirus, 19S gene/polyA
FT                   -> pSBC-2 2860bp"
FT   promoter        complement(5..353)
FT                   /note="from SV40"
FT   enhancer        complement(5..353)
FT                   /note="from SV40"
FT   mRNA            complement(363..421)
FT                   /note="RNA poliovirus type 1 19S"
FT   misc_feature    complement(441..492)
FT                   /note="MCS pUC8 polylinker"
FT   polyA_signal    complement(494..730)
FT                   /note="PLA poliovirus type 1 (VP1)"
XX
SQ   Sequence 2860 BP; 726 A; 708 C; 686 G; 740 T; 0 other;
     agctagcttc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccctcga
     ggaactggaa aaccagaaag ttaactggta agtttagtct ttttgtcttt tatttcaggt
     cccggatcga attgcggccg cgaattcgag ctcgcccggg gatcctctag agtcgacctg
     cagcccaagc ttcgatccag acatgataag atacattgat gagtttggac aaaccacaac
     tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     ggctgattat gatcctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgtc gggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattggt
     cgatcgacca attctcatgt ttgacagctt atcatcgata
//