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ID PSC101 preliminary; circular DNA; PRO; 9263 BP.
XX
AC X01654; ATCC37032;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Salmonella plasmid vector pSC101 - complete.
XX
KW cloning vector.
XX
OS Salmonella typhimurium
OC Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
OC Facultatively anaerobic rods; Enterobacteriaceae; Salmonella.
XX
RN [1]
RP 1-9263
RC pSC101 from R6-5, Salmonella tet gene
RC [pSC101::f2 from pSC101 & lambda, EcoRI fragment f2]
RA Bernardi A., Bernardi F.;
RT "Complete sequence of pSC101";
RL Nucleic Acids Res. 12:9415-9426(1984).
XX
RN [2]
RC pSC101 from R6-5, Salmonella tet gene
RA Cohen S.N., Chang A.C.;
RT "Revised interpretation of the origin of the pSC101 plasmid";
RL J. Bacteriol. 132:734-737(1977).
XX
RN [3]
RC pSC205 from pSC105
RC pSC201 from pSC205
RA Kretschmer P.J., Chang A.C.Y., Cohen S.N.;
RT "Indirect selection of bacterial plasmids lacking identifiable
RT phenotypic properties";
RL J. Bacteriol. 124:225-231(1975).
XX
RN [4]
RC pSC101 from R6-5, Salmonella tet gene
RC pSC102 from R6-5, Salmonella kan gene
RC pSC105 from pSC101 & pSC102
RC pSC109 from pSC101 & RSF1010
RC [pSC204 from pSC101 & R6-5]
RC [pSC84 from pACYC184 & R6-5]
RA Cohen S.N., Chang A.C.Y., Boyer H.W., Helling R.B.;
RT "Construction of biologically functional bacterial plasmids in vitro";
RL Proc. Natl. Acad. Sci. U.S.A. 70:3240-3244(1973).
XX
RN [5]
RC RSF1030 from E. coli
RC R648 from E. coli
RA Heffron F., Sublett R., Hedges R.W., Jacob A., Falkow S.;
RT "Origin of the TEM beta-lactamase gene found on plasmids";
RL J. Bacteriol. 122:250-256(1975).
XX
RN [6]
RC p15A, p15B, p15C from E. coli
RA Ikeda H., Inuzuka M., Tomizawa J.;
RT "PI-like plasmid in Escherichia coli";
RL J. Mol. Biol. 50:457-470(1970).
XX
RN [7]
RC pSC50 from R1-19, Tn3/Tn4
RC pSC175 from pSC101 & pSC50, Tn3 or TnA
RA Kopecko D.J., Cohen S.N.;
RT "Site-specific recA-independent recombination between bacterial
RT plasmids: involvement of pailindromes at the recombinational loci";
RL Proc. Natl. Acad. Sci. U.S.A. 72:1373-1377(1975).
XX
RN [8]
RC pSC101::f2 from pSC101 & lambda, cI857 gene EcoRI fragment f2 imm
RA Bernardi A., Bernardi G.;
RT "Cloning of all EcoRI fragments from phage lambda in E. coli";
RL Nature 264:89-90(1976).
XX
RN [9]
RC RSF1030 from E. coli
RA Crosa J.H., Luttrop L.K., Falkow S.;
RT "Nature of R-factor replication in the presence of chloramphenicol";
RL Proc. Natl. Acad. Sci. U.S.A. 72:654-658(1975).
XX
CC A general purpose plasmid vector. (ATCC staff)
CC Medium is 1273 LB plus tetracycline.
CC NM (pSC101)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (Salmonella)
CC CP (low)
CC FN (cloning)
CC SE ()
CC PA (pMB1)
CC BR ()
CC OF (pSC201)(pKB412 from pSC201 & pBR345)(pKB413 from pKB412 & pBR350)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. Salmonella typhimurium
FT -> R6
FT 1. R6, Tn10 tet gene
FT insertion
FT 2. IS3
FT -> R6-5 [no tet expression]
FT 1. R6-5
FT 2. S.panama SP219 tet gene
FT -> pSC101 9263bp"
FT misc_binding 0..0
FT /note="SIT unique EcoRI"
FT CDS 0..0
FT /note="ANT Salmonella tetracycline resistance gene
FT (tet)"
FT misc_feature 2024..2232
FT /note="Salmonella typhimurium insertion sequence
FT element IS101"
FT CDS complement(2342..2923)
FT /note="GEN Salmonella gene for formation
FT of relaxation complex (194 aa), potential"
FT CDS complement(2939..4051)
FT /note="GEN Salmonella gene for formation
FT of relaxation complex (371 aa), potential"
FT rep_origin 4071..4115
FT /note="ORI Salmonella pSC101;
FT oriT, region of dyad symmetry; potential"
FT misc_feature 6916..7972
FT /note="Salmonella typhimurium insertion sequence
FT element IS102"
XX
SQ Sequence 9263 BP; 2310 A; 2432 C; 2308 G; 2213 T; 0 other;
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
taaagtctgg aaacgcggaa gtcccctacg tgctgctgaa gttgcccgca acagagagtg
gaaccaaccg gtgataccac gatactatga ctgagagtca acgccatgag cggcctcatt
tcttattctg agttacaaca gtccgcaccg ctgccggtag ctacttgact atccggctgc
actagccctg cgtcagatgg ctctgatcca aggcaaactg ccaaaatatc tgctggcacc
ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac
cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc
tctggtgccg ccctatccct ttgtgcagct tgccacgctc aaaggggttt gaggtccaac
cgtacgaaaa cgtacggtaa gaggaaaatt atcgtctgaa aaatcgatta gtagacaaga
aagtccgtta agtgccaatt ttcgattaaa aagacaccgt tttgatggcg ttttccaatg
tacattatgt ttcgatatat cagacagtta cttcactaac gtacgttttc gttctattgg
ccttcagacc ccatatcctt aatgtccttt atttgctggg gttatcagat ccccccgaca
cgtttaatta atgctttctc cgccggagat cgacgcacag gcttctgtgt ctatgatgtt
atttcttaat aatcatccag gtattctctt tatcaccata cgtagtgcga gtgtccacct
taacgcaggg ctttccgtca cagcgcgata tgtcagccag cggggctttc ttttgccaga
ccgcttccat cctctgcatt tcagcaatct ggctataccc gtcattcata aaccacgtaa
atgccgtcac gcaggaagcc aggacgaaga atatcgtcag tacaagataa atcgcggatt
tccacgtata gcgtgacatc tcacgacgca tttcatggat catcgctttc gccgtatcgg
cagcctgatt cagcgcttct gtcgccggtt tctgctgtgc taatccggct tgtttcagtt
ctttctcaac ctgagtgagc gcggaactca ccgatttcct gacggtgtca gtcatattac
cggacgcgct gtccagctca cgaatgaccc tgctcagcgt ttcactttgc tgctgtaatt
gtgatgaggc ggcctgaaac tgttctgtca gagaagtaac acgcttttcc agcgcctgat
gatgcccgat aagggcggca atttgtttaa tttcgtcgct catacaaaat cctgcctatc
gtgagaatga ccagccttta tccggcttct gtcgtatctg ttcggcgagt cgctgtcgtt
ctttctcctg ctgacgctgt ttttccgcca gacgttcgcg ctctctctgc ctttccatct
cctgatgtat cccctggaac tccgccatcg catcgttaac aagggactga agatcgattt
cttcctgtat atccttcatg gcatcactga ccagtgcgtt cagcttgtca ggctcttttt
caaaatcaaa cgttctgccg gaatgggatt cctgctcagg ctctgacttc agctcctgtt
ttagcgtcag agtatccctc tcgctgaggg cttcccgtaa cgaggtagtc acgtcaatta
cgctgtcacg ttcatcacgg gactgctgca cctgcctttc agcctccctg cgctcaagaa
tggcctgtag ctgctcagta tcgaatcgct gaacctgacc cgcgcccaga tgccgctcag
gctcacggtc aatgccctgc gccttcaggg aacgggaatc aacccggtca gcgtgctgat
accgttcaag gtgcttattc tggaggtcag cccagcgtct ccctctgggc aacaaggtat
tctttgcgtt cggtcggtgt ttccccgaaa cgtgcctttt ttgcgccacc gcgtccggct
ctttggtgtt agcccgttta aaatactgct cagggtcacg gtgaataccg tcattaatgc
gttcagagaa catgatatgg gcgtggggct gctcgccacc ggctatcgct gctttcggat
tatggatagc gaactgatag gcatggcggt cgccaatttc ctgttggaca aaatcgcgga
caagctcaag acgttgttcg ggttttaact cacgcggcag ggcaatctcg atttcacggt
aggtacagcc gttggcacgt tcagacgtgt cagcggcttt ccagaactcg gacggtttat
gcgctgccca cgccggcata ttgccggact ccttgtgctc aaggtcggag tctttttcac
gggcatactt tccctcacgc gcaatataat cggcatgagg agaggcactg ccttttccgc
cggtttttac gctgagatga taggatgcca tcgtgtttta tcccgctgaa gggcgcacgt
ttctgaacga agtgaagaaa gtctaagtgc gccctgataa ataaaagagt tatcagggat
tgtagtggga tttgacctcc tctgccatca tgagcgtaat cattccgtta gcattcagga
ggtaaacagc atgaataaaa gcgaaaaaac aggaacaatg ggcagcagaa agagtgcagt
atattcgcgg cttaaagtcg ccgaatgagc aacagaaact tatgctgata ctgacggata
aagcagataa aacagcacag gatatcaaaa cgctgtccct gctgatgaag gctgaacagg
cagcagagaa agcgcaggaa gccagagcga aagtcatgaa cctgatacag gcagaaaagc
gagccgaagc cagagccgcc cgtaaagccc gtgaccatgc tctgtaccag tctgccggat
tgcttatcct ggcgggtctg gttgacagta agacgggtaa gcctgttgat gataccgctg
ccttactggg tgcattagcc agtctgaatg acctgtcacg ggataatccg aagtggtcag
actggaaaat cagagggcag gaactgctga acagcaaaaa gtcagatagc accacatagc
agacccgcca taaaacgccc tgagaagccc gtgacgggct tttcttgtat tatgggtagt
ttccttgcat gaatccataa aaggcgcctg tagtgccatt tacccccatt cactgccaga
gccgtgagcg cagcgaactg aatgtcacga aaaagacagc gactcaggtg cctgatggtc
ggagacaaaa ggaatattca gcgatttgcc cgagcttgcg agggtgctac ttaagccttt
agggttttaa ggtctgtttt gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata
ctgcggaact gactaaagta gtgagttata cacagggctg ggatctattc tttttatctt
tttttattct ttctttattc tataaattat aaccacttga atataaacaa aaaaaacaca
caaaggtcta gcggaattta cagagggtct agcagaattt acaagttttc cagcaaaggt
ctagcagaat ttacagatac ccacaactca aaggaaaagg actagtaatt atcattgact
agcccatctc aattggtata gtgattaaaa tcacctagac caattgagat gtatgtctga
attagttgtt ttcaaagcaa atgaactagc gattagtcgc tatgacttaa cggagcatga
aaccaagcta attttatgct gtgtggcact actcaacccc acgattgaaa accctacaag
gaaagaacgg acggtatcgt tcacttataa ccaatacgct cagatgatga acatcagtag
ggaaaatgct tatggtgtat tagctaaagc aaccagagag ctgatgacga gaactgtgga
aatcaggaat cctttggtta aaggctttga gattttccag tggacaaact atgccaagtt
ctcaagcgaa aaattagaat tagtttttag tgaagagata ttgccttatc ttttccagtt
aaaaaaattc ataaaatata atctggaaca tgttaagtct tttgaaaaca aatactctat
gaggatttat gagtggttat taaaagaact aacacaaaag aaaactcaca aggcaaatat
agagattagc cttgatgaat ttaagttcat gttaatgctt gaaaataact accatgagtt
taaaaggctt aaccaatggg ttttgaaacc aataagtaaa gatttaaaca cttacagcaa
tatgaaattg gtggttgata agcgaggccg cccgactgat acgttgattt tccaagttga
actagataga caaatggatc tcgtaaccga acttgagaac aaccagataa aaatgaatgg
tgacaaaata ccaacaacca ttacatcaga ttcctaccta cgtaacggac taagaaaaac
actacacgat gctttaactg caaaaattca gctcaccagt tttgaggcaa aatttttgag
tgacatgcaa agtaagcatg atctcaatgg ttcgttctca tggctcacgc aaaaacaacg
aaccacacta gagaacatac tggctaaata cggaaggatc tgaggttctt atggctcttg
tatctatcag tgaagcatca agactaacaa acaaaagtag aacaactgtt caccgttaga
tatcaaaggg aaaactgtcc atatgcacag atgaaaacgg tgtaaaaaag atagatacat
cagagctttt acgagttttt ggtgcattta aagctgttca ccatgaacag atcgacaatg
taacagatga acagcatgta acacctaata gaacaggtga aaccagtaaa acaaagcaac
tagaacatga aattgaacac ctgagacaac ttgttacagc tcaacagtca cacatagaca
gcctgaaaca ggcgatgctg cttatcgaat caaagctgcc gacaacacgg gagccagtga
cgcctcccgt ggggaaaaaa tcatggcaat tctggaagaa atagcgcttt cagccggcaa
acctgaagcc ggatctgcga ttctgataac aaactagcaa caccagaaca gcccgtttgc
gggcagcaaa acccgtactt ttggacgttc cggcggtttt ttgtggcgag tggtgttcgg
gcggtgcgcg caagatccat tatgttaaac gggcgagttt acatctcaaa accgcccgct
taacaccatc agaaatcctc agcgcgattt taagcaccaa cccccccccg taacacccaa
atccatactg aaagtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
tcacgcatcc tcccgacaac acagaccatt ccgtggcaaa gcaaaagttc agaatcacca
actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
atgaggcgat tcaggcctgg tatgagtcgg caacaccttc atcacgagga aggccccagc
gctattctga tctcgccatc accaccgttc tggtgattaa acgcgtattc cggctgaccc
tgcgggctgc gcagggtttt attgattcca tttttgccct gatgaacgtt ccgttgcgct
gcccggatta caccagtgtc agtaagcggg caaagtcggt taatgtcagt ttcaaaacgt
ccacccgggg tgaaatcgca cacctggtga ttgattccac cgggctgaag gtctttggtg
aaggcgaatg gaaagtcaga aagcacggca aagagcgccg tcgtatctgg cgaaagttgc
atcttgctgt tgacagcaac acacatgaag ttgtctgtgc agacctgtcg ctgaataacg
tcacggactc agaagccttc ccgggcctta tccggcagac tcacagaaaa atcagggcag
ccgcggcaga cggggcttac gatacccggc tctgtcacga tgaactgcgc cgcaaaaaaa
tcagcgcgct tattcctccc cgaaaaggtg cgggttactg gcccggtgaa tatgcagacc
gtaaccgtgc agtggctaat cagcgaatga ccgggagtaa tgcgcggtgg aaatggacaa
cagattacaa ccgtcgctcg atagcggaaa cggcgatgta ccgggtaaaa cagctgttcg
ggggttcact gacgctgcgt gactacgatg gtcaggttgc ggaggctatg gccctggtac
gagcgctgaa caaaatgacg aaagcaggta tgcctgaaag cgtgcgtatt gcctgaaaac
acaacccgct acgggggaga cttacccgaa atctgattta ttcaacaaag ccgggtgtgg
tgaactacaa agcagacccg ttgaggttat cagttcgatg cacaatcagc agcgcataaa
atatgcacaa gaacaggagc acccttcgca ttaagctgtg gtggtaacaa gtagtgccgg
gctaccatca gcgagcatga tgcgctccca cagcattcgc cttggcagta tggaagttcc
tcgctccagt tcgggccggt atccacctcg agtgacccca gccgcccctc atgccaaccg
agcccattaa gcgcagagtc ggccgcaact atctcgggga cgcataaaca cgcagcgatt
taccaaggag ttcggctagg tcaatccgca attccctttg cctggccgga gatgccgcga
gcaaacaatg tgtccggtcg ctaccagatg gactttgtgg gcggaggatt gcaccagacg
cttcagcgca gcgtcgtacg atgaatcgga aggcccggta agcaattgaa gaaccgctga
ccacctccca tccgacaatg caagggtgcc ccgctcccag cgcgacacgg ttgcctgatc
cacacccatt aattcagcta ggtgactttg cttcatatga cgaagcaacc gcgccctccg
cacctgccgc cccctgtcat tcgtcaacat tcttcagcac ctcaatgtcg ttcgtagtga
gcctcatttt ttcaagaccg ccgatgatga gagccaggcc tcgctcgaat gccgcgtccg
gaccgccttc gtagacgatt ttcatcgcgc tctgtaggcg cgccggcatc gtagacgctg
aggtggtcaa ctgatcttcg ccccgctcct cggcgtctgc ctcgctagct tgctgctcaa
gaacagcgcc gacggtgaag tagctgattg ccatcaacgc ataggtcgcg tcacctgccg
aaaagccagc atcgcaaagg aagcgaagct gcgcgtcggc tttttccatc tgcggcgcgg
ctggccgcgt cccggcatga atacgcgcgc catcgcgata agcgagcaac gcccgtcgaa
aactgcatgc attgcccttc aggaacgaac gccagtcgtc gtcatccctt ggcgtcgaat
gcgtgtgatt tatcgtcagc atggcttcgg caagtgcgtc gagcaacgca cgcttgttct
tgaaatgcca gtagagcgct ggctgttgca ccccgaggcg ctcagccagt cggcgcgtcg
ttagaccttc catgcccacg tcgttaagca gttcgagcgc ggttcggatc acggcctcgc
gttggagctt gttcattcgc gaa
//