back Return to this vector's summary.
ID   PSC101     preliminary; circular DNA; PRO; 9263 BP.
XX
AC   X01654; ATCC37032;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Salmonella plasmid vector pSC101 - complete.
XX
KW   cloning vector.
XX
OS   Salmonella typhimurium
OC   Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
OC   Facultatively anaerobic rods; Enterobacteriaceae; Salmonella.
XX
RN   [1]
RP   1-9263
RC   pSC101 from R6-5, Salmonella tet gene
RC   [pSC101::f2 from pSC101 & lambda, EcoRI fragment f2]
RA   Bernardi A., Bernardi F.;
RT   "Complete sequence of pSC101";
RL   Nucleic Acids Res. 12:9415-9426(1984).
XX
RN   [2]
RC   pSC101 from R6-5, Salmonella tet gene
RA   Cohen S.N., Chang A.C.;
RT   "Revised interpretation of the origin of the pSC101 plasmid";
RL   J. Bacteriol. 132:734-737(1977).
XX
RN   [3]
RC   pSC205 from pSC105
RC   pSC201 from pSC205
RA   Kretschmer P.J., Chang A.C.Y., Cohen S.N.;
RT   "Indirect selection of bacterial plasmids lacking identifiable
RT   phenotypic properties";
RL   J. Bacteriol. 124:225-231(1975).
XX
RN   [4]
RC   pSC101 from R6-5, Salmonella tet gene
RC   pSC102 from R6-5, Salmonella kan gene
RC   pSC105 from pSC101 & pSC102
RC   pSC109 from pSC101 & RSF1010
RC   [pSC204 from pSC101 & R6-5]
RC   [pSC84 from pACYC184 & R6-5]
RA   Cohen S.N., Chang A.C.Y., Boyer H.W., Helling R.B.;
RT   "Construction of biologically functional bacterial plasmids in vitro";
RL   Proc. Natl. Acad. Sci. U.S.A. 70:3240-3244(1973).
XX
RN   [5]
RC   RSF1030 from E. coli
RC   R648 from E. coli
RA   Heffron F., Sublett R., Hedges R.W., Jacob A., Falkow S.;
RT   "Origin of the TEM beta-lactamase gene found on plasmids";
RL   J. Bacteriol. 122:250-256(1975).
XX
RN   [6]
RC   p15A, p15B, p15C from E. coli
RA   Ikeda H., Inuzuka M., Tomizawa J.;
RT   "PI-like plasmid in Escherichia coli";
RL   J. Mol. Biol. 50:457-470(1970).
XX
RN   [7]
RC   pSC50 from R1-19, Tn3/Tn4
RC   pSC175 from pSC101 & pSC50, Tn3 or TnA
RA   Kopecko D.J., Cohen S.N.;
RT   "Site-specific recA-independent recombination between bacterial
RT   plasmids: involvement of pailindromes at the recombinational loci";
RL   Proc. Natl. Acad. Sci. U.S.A. 72:1373-1377(1975).
XX
RN   [8]
RC   pSC101::f2 from pSC101 & lambda, cI857 gene EcoRI fragment f2 imm
RA   Bernardi A., Bernardi G.;
RT   "Cloning of all EcoRI fragments from phage lambda in E. coli";
RL   Nature 264:89-90(1976).
XX
RN   [9]
RC   RSF1030 from E. coli
RA   Crosa J.H., Luttrop L.K., Falkow S.;
RT   "Nature of R-factor replication in the presence of chloramphenicol";
RL   Proc. Natl. Acad. Sci. U.S.A. 72:654-658(1975).
XX
CC   A general purpose plasmid vector. (ATCC staff)
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pSC101)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Salmonella)
CC   CP (low)
CC   FN (cloning)
CC   SE ()
CC   PA (pMB1)
CC   BR ()
CC   OF (pSC201)(pKB412 from pSC201 & pBR345)(pKB413 from pKB412 & pBR350)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Salmonella typhimurium
FT                   -> R6
FT                   1. R6, Tn10 tet gene
FT                   insertion
FT                   2. IS3
FT                   -> R6-5 [no tet expression]
FT                   1. R6-5
FT                   2. S.panama SP219 tet gene
FT                   -> pSC101 9263bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   CDS             0..0
FT                   /note="ANT Salmonella tetracycline resistance gene
FT                   (tet)"
FT   misc_feature    2024..2232
FT                   /note="Salmonella typhimurium insertion sequence
FT                   element IS101"
FT   CDS             complement(2342..2923)
FT                   /note="GEN Salmonella gene for formation
FT                   of relaxation complex (194 aa), potential"
FT   CDS             complement(2939..4051)
FT                   /note="GEN Salmonella gene for formation
FT                   of relaxation complex (371 aa), potential"
FT   rep_origin      4071..4115
FT                   /note="ORI Salmonella pSC101;
FT                   oriT, region of dyad symmetry; potential"
FT   misc_feature    6916..7972
FT                   /note="Salmonella typhimurium insertion sequence
FT                   element IS102"
XX
SQ   Sequence 9263 BP; 2310 A; 2432 C; 2308 G; 2213 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     taaagtctgg aaacgcggaa gtcccctacg tgctgctgaa gttgcccgca acagagagtg
     gaaccaaccg gtgataccac gatactatga ctgagagtca acgccatgag cggcctcatt
     tcttattctg agttacaaca gtccgcaccg ctgccggtag ctacttgact atccggctgc
     actagccctg cgtcagatgg ctctgatcca aggcaaactg ccaaaatatc tgctggcacc
     ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac
     cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc
     tctggtgccg ccctatccct ttgtgcagct tgccacgctc aaaggggttt gaggtccaac
     cgtacgaaaa cgtacggtaa gaggaaaatt atcgtctgaa aaatcgatta gtagacaaga
     aagtccgtta agtgccaatt ttcgattaaa aagacaccgt tttgatggcg ttttccaatg
     tacattatgt ttcgatatat cagacagtta cttcactaac gtacgttttc gttctattgg
     ccttcagacc ccatatcctt aatgtccttt atttgctggg gttatcagat ccccccgaca
     cgtttaatta atgctttctc cgccggagat cgacgcacag gcttctgtgt ctatgatgtt
     atttcttaat aatcatccag gtattctctt tatcaccata cgtagtgcga gtgtccacct
     taacgcaggg ctttccgtca cagcgcgata tgtcagccag cggggctttc ttttgccaga
     ccgcttccat cctctgcatt tcagcaatct ggctataccc gtcattcata aaccacgtaa
     atgccgtcac gcaggaagcc aggacgaaga atatcgtcag tacaagataa atcgcggatt
     tccacgtata gcgtgacatc tcacgacgca tttcatggat catcgctttc gccgtatcgg
     cagcctgatt cagcgcttct gtcgccggtt tctgctgtgc taatccggct tgtttcagtt
     ctttctcaac ctgagtgagc gcggaactca ccgatttcct gacggtgtca gtcatattac
     cggacgcgct gtccagctca cgaatgaccc tgctcagcgt ttcactttgc tgctgtaatt
     gtgatgaggc ggcctgaaac tgttctgtca gagaagtaac acgcttttcc agcgcctgat
     gatgcccgat aagggcggca atttgtttaa tttcgtcgct catacaaaat cctgcctatc
     gtgagaatga ccagccttta tccggcttct gtcgtatctg ttcggcgagt cgctgtcgtt
     ctttctcctg ctgacgctgt ttttccgcca gacgttcgcg ctctctctgc ctttccatct
     cctgatgtat cccctggaac tccgccatcg catcgttaac aagggactga agatcgattt
     cttcctgtat atccttcatg gcatcactga ccagtgcgtt cagcttgtca ggctcttttt
     caaaatcaaa cgttctgccg gaatgggatt cctgctcagg ctctgacttc agctcctgtt
     ttagcgtcag agtatccctc tcgctgaggg cttcccgtaa cgaggtagtc acgtcaatta
     cgctgtcacg ttcatcacgg gactgctgca cctgcctttc agcctccctg cgctcaagaa
     tggcctgtag ctgctcagta tcgaatcgct gaacctgacc cgcgcccaga tgccgctcag
     gctcacggtc aatgccctgc gccttcaggg aacgggaatc aacccggtca gcgtgctgat
     accgttcaag gtgcttattc tggaggtcag cccagcgtct ccctctgggc aacaaggtat
     tctttgcgtt cggtcggtgt ttccccgaaa cgtgcctttt ttgcgccacc gcgtccggct
     ctttggtgtt agcccgttta aaatactgct cagggtcacg gtgaataccg tcattaatgc
     gttcagagaa catgatatgg gcgtggggct gctcgccacc ggctatcgct gctttcggat
     tatggatagc gaactgatag gcatggcggt cgccaatttc ctgttggaca aaatcgcgga
     caagctcaag acgttgttcg ggttttaact cacgcggcag ggcaatctcg atttcacggt
     aggtacagcc gttggcacgt tcagacgtgt cagcggcttt ccagaactcg gacggtttat
     gcgctgccca cgccggcata ttgccggact ccttgtgctc aaggtcggag tctttttcac
     gggcatactt tccctcacgc gcaatataat cggcatgagg agaggcactg ccttttccgc
     cggtttttac gctgagatga taggatgcca tcgtgtttta tcccgctgaa gggcgcacgt
     ttctgaacga agtgaagaaa gtctaagtgc gccctgataa ataaaagagt tatcagggat
     tgtagtggga tttgacctcc tctgccatca tgagcgtaat cattccgtta gcattcagga
     ggtaaacagc atgaataaaa gcgaaaaaac aggaacaatg ggcagcagaa agagtgcagt
     atattcgcgg cttaaagtcg ccgaatgagc aacagaaact tatgctgata ctgacggata
     aagcagataa aacagcacag gatatcaaaa cgctgtccct gctgatgaag gctgaacagg
     cagcagagaa agcgcaggaa gccagagcga aagtcatgaa cctgatacag gcagaaaagc
     gagccgaagc cagagccgcc cgtaaagccc gtgaccatgc tctgtaccag tctgccggat
     tgcttatcct ggcgggtctg gttgacagta agacgggtaa gcctgttgat gataccgctg
     ccttactggg tgcattagcc agtctgaatg acctgtcacg ggataatccg aagtggtcag
     actggaaaat cagagggcag gaactgctga acagcaaaaa gtcagatagc accacatagc
     agacccgcca taaaacgccc tgagaagccc gtgacgggct tttcttgtat tatgggtagt
     ttccttgcat gaatccataa aaggcgcctg tagtgccatt tacccccatt cactgccaga
     gccgtgagcg cagcgaactg aatgtcacga aaaagacagc gactcaggtg cctgatggtc
     ggagacaaaa ggaatattca gcgatttgcc cgagcttgcg agggtgctac ttaagccttt
     agggttttaa ggtctgtttt gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata
     ctgcggaact gactaaagta gtgagttata cacagggctg ggatctattc tttttatctt
     tttttattct ttctttattc tataaattat aaccacttga atataaacaa aaaaaacaca
     caaaggtcta gcggaattta cagagggtct agcagaattt acaagttttc cagcaaaggt
     ctagcagaat ttacagatac ccacaactca aaggaaaagg actagtaatt atcattgact
     agcccatctc aattggtata gtgattaaaa tcacctagac caattgagat gtatgtctga
     attagttgtt ttcaaagcaa atgaactagc gattagtcgc tatgacttaa cggagcatga
     aaccaagcta attttatgct gtgtggcact actcaacccc acgattgaaa accctacaag
     gaaagaacgg acggtatcgt tcacttataa ccaatacgct cagatgatga acatcagtag
     ggaaaatgct tatggtgtat tagctaaagc aaccagagag ctgatgacga gaactgtgga
     aatcaggaat cctttggtta aaggctttga gattttccag tggacaaact atgccaagtt
     ctcaagcgaa aaattagaat tagtttttag tgaagagata ttgccttatc ttttccagtt
     aaaaaaattc ataaaatata atctggaaca tgttaagtct tttgaaaaca aatactctat
     gaggatttat gagtggttat taaaagaact aacacaaaag aaaactcaca aggcaaatat
     agagattagc cttgatgaat ttaagttcat gttaatgctt gaaaataact accatgagtt
     taaaaggctt aaccaatggg ttttgaaacc aataagtaaa gatttaaaca cttacagcaa
     tatgaaattg gtggttgata agcgaggccg cccgactgat acgttgattt tccaagttga
     actagataga caaatggatc tcgtaaccga acttgagaac aaccagataa aaatgaatgg
     tgacaaaata ccaacaacca ttacatcaga ttcctaccta cgtaacggac taagaaaaac
     actacacgat gctttaactg caaaaattca gctcaccagt tttgaggcaa aatttttgag
     tgacatgcaa agtaagcatg atctcaatgg ttcgttctca tggctcacgc aaaaacaacg
     aaccacacta gagaacatac tggctaaata cggaaggatc tgaggttctt atggctcttg
     tatctatcag tgaagcatca agactaacaa acaaaagtag aacaactgtt caccgttaga
     tatcaaaggg aaaactgtcc atatgcacag atgaaaacgg tgtaaaaaag atagatacat
     cagagctttt acgagttttt ggtgcattta aagctgttca ccatgaacag atcgacaatg
     taacagatga acagcatgta acacctaata gaacaggtga aaccagtaaa acaaagcaac
     tagaacatga aattgaacac ctgagacaac ttgttacagc tcaacagtca cacatagaca
     gcctgaaaca ggcgatgctg cttatcgaat caaagctgcc gacaacacgg gagccagtga
     cgcctcccgt ggggaaaaaa tcatggcaat tctggaagaa atagcgcttt cagccggcaa
     acctgaagcc ggatctgcga ttctgataac aaactagcaa caccagaaca gcccgtttgc
     gggcagcaaa acccgtactt ttggacgttc cggcggtttt ttgtggcgag tggtgttcgg
     gcggtgcgcg caagatccat tatgttaaac gggcgagttt acatctcaaa accgcccgct
     taacaccatc agaaatcctc agcgcgattt taagcaccaa cccccccccg taacacccaa
     atccatactg aaagtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
     tcacgcatcc tcccgacaac acagaccatt ccgtggcaaa gcaaaagttc agaatcacca
     actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
     atgaggcgat tcaggcctgg tatgagtcgg caacaccttc atcacgagga aggccccagc
     gctattctga tctcgccatc accaccgttc tggtgattaa acgcgtattc cggctgaccc
     tgcgggctgc gcagggtttt attgattcca tttttgccct gatgaacgtt ccgttgcgct
     gcccggatta caccagtgtc agtaagcggg caaagtcggt taatgtcagt ttcaaaacgt
     ccacccgggg tgaaatcgca cacctggtga ttgattccac cgggctgaag gtctttggtg
     aaggcgaatg gaaagtcaga aagcacggca aagagcgccg tcgtatctgg cgaaagttgc
     atcttgctgt tgacagcaac acacatgaag ttgtctgtgc agacctgtcg ctgaataacg
     tcacggactc agaagccttc ccgggcctta tccggcagac tcacagaaaa atcagggcag
     ccgcggcaga cggggcttac gatacccggc tctgtcacga tgaactgcgc cgcaaaaaaa
     tcagcgcgct tattcctccc cgaaaaggtg cgggttactg gcccggtgaa tatgcagacc
     gtaaccgtgc agtggctaat cagcgaatga ccgggagtaa tgcgcggtgg aaatggacaa
     cagattacaa ccgtcgctcg atagcggaaa cggcgatgta ccgggtaaaa cagctgttcg
     ggggttcact gacgctgcgt gactacgatg gtcaggttgc ggaggctatg gccctggtac
     gagcgctgaa caaaatgacg aaagcaggta tgcctgaaag cgtgcgtatt gcctgaaaac
     acaacccgct acgggggaga cttacccgaa atctgattta ttcaacaaag ccgggtgtgg
     tgaactacaa agcagacccg ttgaggttat cagttcgatg cacaatcagc agcgcataaa
     atatgcacaa gaacaggagc acccttcgca ttaagctgtg gtggtaacaa gtagtgccgg
     gctaccatca gcgagcatga tgcgctccca cagcattcgc cttggcagta tggaagttcc
     tcgctccagt tcgggccggt atccacctcg agtgacccca gccgcccctc atgccaaccg
     agcccattaa gcgcagagtc ggccgcaact atctcgggga cgcataaaca cgcagcgatt
     taccaaggag ttcggctagg tcaatccgca attccctttg cctggccgga gatgccgcga
     gcaaacaatg tgtccggtcg ctaccagatg gactttgtgg gcggaggatt gcaccagacg
     cttcagcgca gcgtcgtacg atgaatcgga aggcccggta agcaattgaa gaaccgctga
     ccacctccca tccgacaatg caagggtgcc ccgctcccag cgcgacacgg ttgcctgatc
     cacacccatt aattcagcta ggtgactttg cttcatatga cgaagcaacc gcgccctccg
     cacctgccgc cccctgtcat tcgtcaacat tcttcagcac ctcaatgtcg ttcgtagtga
     gcctcatttt ttcaagaccg ccgatgatga gagccaggcc tcgctcgaat gccgcgtccg
     gaccgccttc gtagacgatt ttcatcgcgc tctgtaggcg cgccggcatc gtagacgctg
     aggtggtcaa ctgatcttcg ccccgctcct cggcgtctgc ctcgctagct tgctgctcaa
     gaacagcgcc gacggtgaag tagctgattg ccatcaacgc ataggtcgcg tcacctgccg
     aaaagccagc atcgcaaagg aagcgaagct gcgcgtcggc tttttccatc tgcggcgcgg
     ctggccgcgt cccggcatga atacgcgcgc catcgcgata agcgagcaac gcccgtcgaa
     aactgcatgc attgcccttc aggaacgaac gccagtcgtc gtcatccctt ggcgtcgaat
     gcgtgtgatt tatcgtcagc atggcttcgg caagtgcgtc gagcaacgca cgcttgttct
     tgaaatgcca gtagagcgct ggctgttgca ccccgaggcg ctcagccagt cggcgcgtcg
     ttagaccttc catgcccacg tcgttaagca gttcgagcgc ggttcggatc acggcctcgc
     gttggagctt gttcattcgc gaa
//