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ID   PSC194     preliminary; circular DNA; SYN; 7307 BP.
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AC   ATCC37083;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/Staphylococcus plasmid vector pSC194 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pC194 from S.aureus
RC   pS194 from S.aureus
RC   pSC194 from pS194 & pC194
RA   Lofdahl S., Sjostrom J.E., Philipson L.;
RT   "Characterization of small plasmids from Staphylococcus aureus";
RL   Gene 3:149-159(1978).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   Vector for construction and propagation of hybrid DNA in
CC   Staphylococcus aureus or Bacillus subtilis.
CC   Contains two HindIII sites, one EcoRI site and no sites for BamHI or
CC   SmaI.
CC   Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC   NM (pSC194)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Staphylococcus aureus)(Bacillus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pS194)(pC194)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pS194 HindIII 4397bp 656..656, strep gene
FT                   2. pC194 HindIII 2910bp 2436..2436, cat gene
FT                   -> pSC194 7280bp [2 HindIII, unique EcoRI"
FT   -               1..2435
FT                   /note="pC194 1..2435 2435bp
FT                   HindIII = A^AGCTT"
FT   -               2436..6832
FT                   /note="pS194 656..4397..655 4397bp
FT                   HindIII = A^AGCTT"
FT   -               6833..7307
FT                   /note="pC194 2436..2910 475bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pS194"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pC194"
FT   CDS             0..0
FT                   /note="ANT Bacillus chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT Bacillus streptomycin resistance gene
FT                   (str)"
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SQ   Sequence 7307 BP; 2661 A; 1012 C; 1230 G; 2404 T; 0 other;
     gatcaatttc ttttaagtaa tctaaatccc cattttttaa tttcttttta gcctctttaa
     ataatcctga ataaactaat acctgtttac ctttaagtga tttataaaat gcatcaaaga
     ctttttgatt tatttattaa ataatcacta tctttaccag aatacttagc catttcatat
     aattctttat tattattttg tcttattttt tgaacttgaa cttgtgttat ttctgaaatg
     cccgttacat cacgccataa atctaaccat tcttgttggc taatataata tcttttatct
     gtgaaatacg atttatttac tgcaattaac acatgaaaat gaggattata atcatctctt
     tttttattat atgtaatctc taacttacga acatatccct ttataacact acctactttt
     tttctcttta taagttttct aaaagaatta ttataacgtt ttatttcatt ttctaattca
     tcactcatta cattaggtgt agtcaaagtt aaaaagataa actccttttt ctcttgctgc
     ttaatatatt gcatcatcaa agataaaccc aatgcatctt ttctagcttt tctccaagca
     cagacaggac aaaatcgatt tttacaagaa ttagctttat ataatttctg tttttctaaa
     gttttatcag ctacaaaaga cagaaatgta ttgcaatctt caactaaatc catttgattc
     tctccaatat gacgtttaat aaatttctga aatacttgat ttctttgttt tttctcagta
     tacttttcca tgttataaca cataaaaaca acttagtttt cacaaactat gacaataaaa
     aaagttgctt tttccccttt ctatgtatgt tttttactag tcatttaaaa cgatacatta
     ataggtacga aaaagcaact ttttttgcgc ttaaaaccag tcataccaat aacttaaggg
     taactagcct cgccggcaat agttaccctt attatcaaga taagaaagaa aaggattttt
     cgctacgctc aaatccttta aaaaaacaca aaagaccaca ttttttaatg tggtctttat
     tcttcaacta aagcacccat tagttcaaca aacgaaaatt ggataaagtg ggatattttt
     aaaatatata tttatgttac agtaatattg acttttaaaa aaggattgat tctaatgaag
     aaagcagaca agtaagcctc ctaaattcac tttagataaa aatttaggag gcatatcaaa
     tgaactttaa taaaattgat ttagacaatt ggaagagaaa agagatattt aatcattatt
     tgaaccaaca aacgactttt agtataacca cagaaattga tattagtgtt ttataccgaa
     acataaaaca agaaggatat aaattttacc ctgcatttat tttcttagtg acaagggtga
     taaactcaaa tacagctttt agaactggtt acaatagcga cggagagtta ggttattggg
     ataagttaga gccactttat acaatttttg atggtgtatc taaaacattc tctggtattt
     ggactcctgt aaagaatgac ttcaaagagt tttatgattt atacctttct gatgtagaga
     aatataatgg ttcggggaaa ttgtttccca aaacacctat acctgaaaat gctttttctc
     tttctattat tccatggact tcatttactg ggtttaactt aaatatcaat aataatagta
     attaccttct acccattatt acagcaggaa aattcattaa taaaggtaat tcaatatatt
     taccgctatc tttacaggta catcattctg tttgtgatgg ttatcatgca ggattgttta
     tgaactctat tcaggaattg tcagataggc ctaatgactg gcttttataa tatgagataa
     tgccgactgt actttttaca gtcggttttc taatgtcact aacctgcccc gttagttgaa
     gaaggttttt atattacagc tccagatcca tatccttctt tttctgaacc gacttctcct
     ttttcgcttc tttattccaa ttgctttatt gacgttgagc ctcggaaccc ttaacaatcc
     caaaacttgt cgaatggtcg gcttaatagc tcacgctatg ccgacattcg tctgcaagtt
     tagttaaggg ttcttctcaa cgcacaataa attttctcgg cataaatgcg tggtctaatt
     tttattttta ataaccttga tagcaaaaaa tgccattcca atacaaaacc acatacctat
     aatcgataac cacataacag tcataaaacc actccttttt aacaaacttt atcacaagaa
     atatttaaat tttaaatgcc tttattttga attttaaggg gcattttaaa gatttagggg
     taaatcatat agttttatgc ctaaaaacct acagaagctt tgttgtgaat tatttaaata
     ttattcaaat aaagtaagtt gtttaggaaa ttataactat ccaaattacg aaaaaaatat
     tgagaatttt attcgtaata attatgagaa ttaattttat tagcaaccac atttttggtt
     gcttttgttt tgattttgaa tttggttttg aactatgagt ggctagcatt tttccactca
     ttttttgcgt tagcaaaaac ataaagggtc tgggattaat cccagcaagc cggtatattc
     agaacgaagt ggctagaata tacgacgctt gccaaaccac tatattactt gagaaaatat
     agattatatt ataagctata tttatgaaaa ggaatatttt tcgtcagaaa ggtggtggga
     cttgtgaacg aacataaaga taatttggct agcgatttgt ctgttggggg aaaccgtaaa
     cccaaccgca aagagccaaa acaaattagt ttcagagtga gtgaacagga atattcaaag
     ttgaaacagt cggcagaaac tttgaatatg acagtgccta atttcgttaa gaaaaaggca
     caaggcagtc gattagtgtc gccaaaatta gacaaagaaa cgcgacaatc gattgccaaa
     gatttaagtc atttgggtag taatgtgaat cagattgcta agtggtttaa tcaaaataaa
     gataaggcta cacatttacc agaagagaaa tatcatgatt tagaaaagca atttgatgat
     gtgaaaaagg agttgcataa gatatggcaa caactaaatt aggcaatacg aagtcggcaa
     gtcgtgccat taattacgca gaagaacgtg cagaagaaaa aagcggctta aattgtgatg
     ttgattatgc taaatcatat tttaagcaaa ctagggcgtt atatggcaaa gagaatggtg
     ttcaagcaca tacagtcatt caatctttta agcctggcga agtaacagct aaagagtgta
     atgagatagg tttagaatta gctaaaaaaa ttgcaccaga ttatcaagtt gcagtttata
     cacatacaga caaagaccat tatcacaatc acattattat taattcagta aatttagaaa
     caggcaacaa gtatcagtca aacaaagaac aacgtgattt tataaaaaag gcgaatgatc
     agttatgtga agaacgtggt ttatctgtgc cagaaaagtc atcagaaatc agatatacgt
     tggcagaaca gaacatgata gataaagata aaaggtcatg gaaaaatgat ataagaatgg
     cagttgaaga aacaaaggat aacgcagtag cgtttgaaga atttaatact ttgctaaaag
     aaaaaggtgt tgaaatcacg agagtaacga aaaacaacgt aacgtataga cacattgaag
     aagataaaaa agtacgtggg aataagttgg gcgattctta tgacaaagga gtgattgaaa
     atggctttgc aatcgagaaa ttcagacgag aacgagaaga agaacgagaa tacgatgaat
     atgcagacac gttcgaagtt gactgggacg cattcgcaga aaattcagaa gaccttagaa
     aacgaagaat tgcaagaact gaagaaacaa aacaagctag taataaaata tatatcagag
     atgaaagaac aacaggactt gagagaaaag gaattgcagg caatcaagtc gagtttgaaa
     aagacgacgg aggactttca aggtagaggt aaaaaagtcc ataatgattt tgttcgtttg
     ttacaaaaga atttaaatcg agtgaatgca gaagatattg agatagaggt tcgaaaagat
     gtttatggag ttcgagaaga aattcattcg atgttgcgtg aagttaaaca atcacatgaa
     cattatcaaa aaagacaaaa gcaattattt actggtattg gtgcaatgtt attagtgttt
     atgctttttg ctttgattat gacgattggc agagatttta tgggcttttt acatgtagat
     gtattacaga atgccatagc aagcaaaata aaggcttctg agggctttat ttcagtttta
     tggtacattg cttatggttt accttatata ctcgctatag ggctatttat ttggttgtat
     gagtggataa gagcaagatt ccatgattaa acaaagtatg atcaggcttt tatgccgaga
     aaatttattt atattgagaa gtacccttaa ctaaacttgc agacgaatgt cgcatagcgt
     gagtattagc ccgaccattc gacaagtttt gggtttgtta agggtacaga ggctcaatgt
     aaataaagca attggaataa agcaacaaag ggggttgaag aaaatgagta agaagaaaaa
     gattaattta gcaatatcaa ttgctcaagt agtagtgcag gcgttaatac tttataacac
     ttatcagcaa tataaatatt ctaaaaaata ttcagcctaa taaaaaaaga cttagtctga
     atagaccaag ccttttgtta gtgttatatt aatatgcaaa taaaaaaaca aaaaaagaag
     tcgctcactc cctgaccaaa gtttgtgaat gcgacttacc ttaaagaaaa acagttggtg
     tttttttctt aatttttgta tatctagata ttaaactgat attggtttat tcttcaagat
     atatattcgg gtgagcgact tctttaaaca aaataaggag tcgatttttt atgagtaaaa
     aagcagaaga aattcaggca aaacaaagtt tggaaaagga aaactcaaat ttttctaaaa
     ccggatactc taatagccgg ttaaaccgac atattatgta cacccccgaa ccaaaattac
     actttgatgc tatgacaatt gttgggaatc ttaataagaa caatgctcac aaactgtctg
     aatttatgag tattgcgcca caaattagac tttgggatat attacaaacc aaatttaaag
     ctaaggctct acaagaaaaa gtttacatcg aatatgataa agtaaaagca gatgcgtggg
     atagacgtaa tatgcgtgtt gaatttaatc ctaataaact tacgcatgaa gaaatgcttt
     ggttaaaaca aaacatcatt gactacatgg aagatgatgg ttttacaaga ttagatttgg
     cttttgattt tgaagacgat ttgagcgatt attatgcgat gactgataaa tcagttaaga
     aaactatttt ttatggtcgt aacggtaaac cagaaacaaa atattttggt gttcgtgaca
     gtgatagatt tattagaatt tataataaaa aacaagaacg taaggataat gcagacattg
     aagttatgtc tgaacactta tggcgtgtag aaattgaatt aaaaagagat atggttgatt
     attggaacga ttgttttaat gatttacata ttttgaaacc agattggtct agtttagaaa
     aagtaaaaga ccaagcaatg atttatatgc taattcatga agaaagtaca tggggtaaat
     tagaaagacg tacaaaaaat aaatacagag aaatgttaaa aagtatttca gaaattgatt
     taacggactt aatgaaattg actttaaaag agaatgaaaa acaattgcaa aagcagattg
     aattttggca gcgtgaattt agattttggg agtgaaaaaa caatgaggac tgaaaaagaa
     attttaaatt tagtttctga atttgcttat caacgaagca atgtaaaaat tattgctctc
     gagggttcaa gaactaatga aaatataaaa aaagataagt ttcaagatta tgattttgct
     tttttcgtat cagatattga gtatttcaca catgaagaaa gttggttaag tttatttgga
     gaattattgt ttatacagaa accagaagat atggaattat tcccacctga tttagattat
     ggttacagtt atataatgta ttttaaagat ggcataaaaa tggatattac attaattaat
     ttaaaagatt taaatcgtta ttttagtgat tctgatggtc ttgtaaaaat tttagttgat
     aaagataatt tagtaactca agaaattgtt ccagatgact caaattattg gttaaaaaaa
     ccaacagaac gagaatttta tgattgctgt aatgagtttt ggagtgtctc aacgtatgta
     gcaaagggtg tttttagaag agaaatatta tttgctttag atcatttcaa taatatttta
     cgtcctgaat tattaagaat gatttcttgg tatattggct ttaatagggg ttttgatttt
     agtttaggaa agaattataa gtttataaac aaatatttaa ctgataaaga attcaatatg
     cttttagcta cttttgagat gaatggatat agaaagacat accaatcttt taagctttta
     aaaagcaaat atgagccaaa taaatatatt ctaattctac aaacaaaaat ttgagcaaat
     tcagtgtcga ttttttaaga cactgcccag ttacatgcaa attaaaattt tcatgatttt
     ttatagttcc taacagggtt aaaatttgta taacgaaagt ataatgttta tataacgtta
     gtataataaa gcattttaac attatacttt tgataatcgt ttatcgtcgt catcacaata
     acttttaaaa tactcgtgca taattcacgc tgacctccca ataactacat ggtgttatcg
     ggaggtcagc tgttagcact tatattttgt tattgttctt cctcgatttc gtctatcatt
     ttgtgattaa tttctctttt ttcttgttct gttaagtcat aaagttcact agctaaatac
     tctttttgtt tccaaatata aaaaatttga tagatatatt acggttg
//