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ID   PSE101     preliminary; circular DNA; SYN; 10804 BP.
XX
AC   IG9840;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pSE101 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSE100 from pBR322 & E.coli umuC gene
RC   pSE101 from pSC101 & pSE100, neo gene
RC   lambda SE14 from lambda & E.coli umuC gene
RC   pSE110, pSE111, pSE112, pSE113 from pSE101 & lambda SE14
RC   pSE114, pSE115 from pSE101 & lambda SE14
RC   pSE116 from pSE114 & pSE115
RC   pSE137 from pSE116 & pDPT427
RC   pSE140 from pMC903 & pSE110
RC   pSE152 from pRB160 & pDPT427
RC   pSE151 from pBR322 & pDPT427
RA   Elledge S.J., Walker G.C.;
RT   "Proteins required for ultraviolet light and chemical mutagenesis";
RL   J. Mol. Biol. 164:175-192(1983).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pSE101)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli AB1157)(E.coli)(E.coli LE392)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(Tn5)(pSC101)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. Tn5 BglII-BamHI 1541bp 1516..3057, kan/neo gene
FT                   -> pSE100 5902bp
FT                   1. pSC101 BamHI-HindIII 8917bp 376..9263..30, ori
FT                   2. pSE100 HindIII-BamHI 1887bp, Tn5 kan/neo gene
FT                   \ 30..376..-..376/Tn5 1861bp 1196..3057
FT                   -> pSE101 10804bp [no tet, no amp]"
FT   -               1..29
FT                   /note="pSC101 1..29 29bp
FT                   HindIII = A^AGCTT"
FT   -               30..375
FT                   /note="pBR322 30..375 346bp
FT                   BamHI = G^GATCC
FT                   BglII = A^GATCT"
FT   -               376..1916
FT                   /note="Tn5 1516..3056 1541bp
FT                   BamHI = G^GATCC"
FT   -               1917..10804
FT                   /note="pSC101 376..9263 8888bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pSC101 (compatible with ColE1
FT                   and pDPT427)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
XX
SQ   Sequence 10804 BP; 2578 A; 2891 C; 2804 G; 2531 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccgg
     gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt
     ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc agcggctatc cgcgcatcca
     tgcccccgaa ctgcaggagt ggggaggcac gatggccgct ttggtcgacc cggacgggac
     gctcctgcgc ctgatacaga acgaattgct tgcaggcatc tcatgagtgt gtcttcccgt
     tttccgcctg aggtcactgc gtggatggag cgctggcgcc tgctgcgcga cggcgagctg
     ctcaccaccc actcgagctg gatacttccc gtccgccagg gggacatgcc ggcgatgctg
     aaggtcgcgc gcattcccga tgaagaggcc ggttaccgcc tgttgacctg gtgggacggg
     cagggcgccg cccgagtctt cgcctcggcg gcgggcgctc tgctcatgga gcgcgcgtcc
     ggggccgggg accttgcaca gatagcgtgg tccggccagg acgacgaggc ttgcaggatc
     ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     ttcctaatgc aggagtcgca taagggagag cgtcgaccga tgcccttgag agccttcaac
     ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag
     gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg
     cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag
     gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg
     cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc
     gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga
     tcgctcgcgg ctcttaccag cctaacttcg atcactggac cgctgatcgt cacggcgatt
     tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac
     cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg
     gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
     tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca
     tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca
     tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
     agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
     cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
     agtcccctac gtgctgctga agttgcccgc aacagagagt ggaaccaacc ggtgatacca
     cgatactatg actgagagtc aacgccatga gcggcctcat ttcttattct gagttacaac
     agtccgcacc gctgccggta gctacttgac tatccggctg cactagccct gcgtcagatg
     gctctgatcc aaggcaaact gccaaaatat ctgctggcac cggaagtcag cgccctgcac
     cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
     tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtgcc gccctatccc
     tttgtgcagc ttgccacgct caaaggggtt tgaggtccaa ccgtacgaaa acgtacggta
     agaggaaaat tatcgtctga aaaatcgatt agtagacaag aaagtccgtt aagtgccaat
     tttcgattaa aaagacaccg ttttgatggc gttttccaat gtacattatg tttcgatata
     tcagacagtt acttcactaa cgtacgtttt cgttctattg gccttcagac cccatatcct
     taatgtcctt tatttgctgg ggttatcaga tccccccgac acgtttaatt aatgctttct
     ccgccggaga tcgacgcaca ggcttctgtg tctatgatgt tatttcttaa taatcatcca
     ggtattctct ttatcaccat acgtagtgcg agtgtccacc ttaacgcagg gctttccgtc
     acagcgcgat atgtcagcca gcggggcttt cttttgccag accgcttcca tcctctgcat
     ttcagcaatc tggctatacc cgtcattcat aaaccacgta aatgccgtca cgcaggaagc
     caggacgaag aatatcgtca gtacaagata aatcgcggat ttccacgtat agcgtgacat
     ctcacgacgc atttcatgga tcatcgcttt cgccgtatcg gcagcctgat tcagcgcttc
     tgtcgccggt ttctgctgtg ctaatccggc ttgtttcagt tctttctcaa cctgagtgag
     cgcggaactc accgatttcc tgacggtgtc agtcatatta ccggacgcgc tgtccagctc
     acgaatgacc ctgctcagcg tttcactttg ctgctgtaat tgtgatgagg cggcctgaaa
     ctgttctgtc agagaagtaa cacgcttttc cagcgcctga tgatgcccga taagggcggc
     aatttgttta atttcgtcgc tcatacaaaa tcctgcctat cgtgagaatg accagccttt
     atccggcttc tgtcgtatct gttcggcgag tcgctgtcgt tctttctcct gctgacgctg
     tttttccgcc agacgttcgc gctctctctg cctttccatc tcctgatgta tcccctggaa
     ctccgccatc gcatcgttaa caagggactg aagatcgatt tcttcctgta tatccttcat
     ggcatcactg accagtgcgt tcagcttgtc aggctctttt tcaaaatcaa acgttctgcc
     ggaatgggat tcctgctcag gctctgactt cagctcctgt tttagcgtca gagtatccct
     ctcgctgagg gcttcccgta acgaggtagt cacgtcaatt acgctgtcac gttcatcacg
     ggactgctgc acctgccttt cagcctccct gcgctcaaga atggcctgta gctgctcagt
     atcgaatcgc tgaacctgac ccgcgcccag atgccgctca ggctcacggt caatgccctg
     cgccttcagg gaacgggaat caacccggtc agcgtgctga taccgttcaa ggtgcttatt
     ctggaggtca gcccagcgtc tccctctggg caacaaggta ttctttgcgt tcggtcggtg
     tttccccgaa acgtgccttt tttgcgccac cgcgtccggc tctttggtgt tagcccgttt
     aaaatactgc tcagggtcac ggtgaatacc gtcattaatg cgttcagaga acatgatatg
     ggcgtggggc tgctcgccac cggctatcgc tgctttcgga ttatggatag cgaactgata
     ggcatggcgg tcgccaattt cctgttggac aaaatcgcgg acaagctcaa gacgttgttc
     gggttttaac tcacgcggca gggcaatctc gatttcacgg taggtacagc cgttggcacg
     ttcagacgtg tcagcggctt tccagaactc ggacggttta tgcgctgccc acgccggcat
     attgccggac tccttgtgct caaggtcgga gtctttttca cgggcatact ttccctcacg
     cgcaatataa tcggcatgag gagaggcact gccttttccg ccggttttta cgctgagatg
     ataggatgcc atcgtgtttt atcccgctga agggcgcacg tttctgaacg aagtgaagaa
     agtctaagtg cgccctgata aataaaagag ttatcaggga ttgtagtggg atttgacctc
     ctctgccatc atgagcgtaa tcattccgtt agcattcagg aggtaaacag catgaataaa
     agcgaaaaaa caggaacaat gggcagcaga aagagtgcag tatattcgcg gcttaaagtc
     gccgaatgag caacagaaac ttatgctgat actgacggat aaagcagata aaacagcaca
     ggatatcaaa acgctgtccc tgctgatgaa ggctgaacag gcagcagaga aagcgcagga
     agccagagcg aaagtcatga acctgataca ggcagaaaag cgagccgaag ccagagccgc
     ccgtaaagcc cgtgaccatg ctctgtacca gtctgccgga ttgcttatcc tggcgggtct
     ggttgacagt aagacgggta agcctgttga tgataccgct gccttactgg gtgcattagc
     cagtctgaat gacctgtcac gggataatcc gaagtggtca gactggaaaa tcagagggca
     ggaactgctg aacagcaaaa agtcagatag caccacatag cagacccgcc ataaaacgcc
     ctgagaagcc cgtgacgggc ttttcttgta ttatgggtag tttccttgca tgaatccata
     aaaggcgcct gtagtgccat ttacccccat tcactgccag agccgtgagc gcagcgaact
     gaatgtcacg aaaaagacag cgactcaggt gcctgatggt cggagacaaa aggaatattc
     agcgatttgc ccgagcttgc gagggtgcta cttaagcctt tagggtttta aggtctgttt
     tgtagaggag caaacagcgt ttgcgacatc cttttgtaat actgcggaac tgactaaagt
     agtgagttat acacagggct gggatctatt ctttttatct ttttttattc tttctttatt
     ctataaatta taaccacttg aatataaaca aaaaaaacac acaaaggtct agcggaattt
     acagagggtc tagcagaatt tacaagtttt ccagcaaagg tctagcagaa tttacagata
     cccacaactc aaaggaaaag gactagtaat tatcattgac tagcccatct caattggtat
     agtgattaaa atcacctaga ccaattgaga tgtatgtctg aattagttgt tttcaaagca
     aatgaactag cgattagtcg ctatgactta acggagcatg aaaccaagct aattttatgc
     tgtgtggcac tactcaaccc cacgattgaa aaccctacaa ggaaagaacg gacggtatcg
     ttcacttata accaatacgc tcagatgatg aacatcagta gggaaaatgc ttatggtgta
     ttagctaaag caaccagaga gctgatgacg agaactgtgg aaatcaggaa tcctttggtt
     aaaggctttg agattttcca gtggacaaac tatgccaagt tctcaagcga aaaattagaa
     ttagttttta gtgaagagat attgccttat cttttccagt taaaaaaatt cataaaatat
     aatctggaac atgttaagtc ttttgaaaac aaatactcta tgaggattta tgagtggtta
     ttaaaagaac taacacaaaa gaaaactcac aaggcaaata tagagattag ccttgatgaa
     tttaagttca tgttaatgct tgaaaataac taccatgagt ttaaaaggct taaccaatgg
     gttttgaaac caataagtaa agatttaaac acttacagca atatgaaatt ggtggttgat
     aagcgaggcc gcccgactga tacgttgatt ttccaagttg aactagatag acaaatggat
     ctcgtaaccg aacttgagaa caaccagata aaaatgaatg gtgacaaaat accaacaacc
     attacatcag attcctacct acgtaacgga ctaagaaaaa cactacacga tgctttaact
     gcaaaaattc agctcaccag ttttgaggca aaatttttga gtgacatgca aagtaagcat
     gatctcaatg gttcgttctc atggctcacg caaaaacaac gaaccacact agagaacata
     ctggctaaat acggaaggat ctgaggttct tatggctctt gtatctatca gtgaagcatc
     aagactaaca aacaaaagta gaacaactgt tcaccgttag atatcaaagg gaaaactgtc
     catatgcaca gatgaaaacg gtgtaaaaaa gatagataca tcagagcttt tacgagtttt
     tggtgcattt aaagctgttc accatgaaca gatcgacaat gtaacagatg aacagcatgt
     aacacctaat agaacaggtg aaaccagtaa aacaaagcaa ctagaacatg aaattgaaca
     cctgagacaa cttgttacag ctcaacagtc acacatagac agcctgaaac aggcgatgct
     gcttatcgaa tcaaagctgc cgacaacacg ggagccagtg acgcctcccg tggggaaaaa
     atcatggcaa ttctggaaga aatagcgctt tcagccggca aacctgaagc cggatctgcg
     attctgataa caaactagca acaccagaac agcccgtttg cgggcagcaa aacccgtact
     tttggacgtt ccggcggttt tttgtggcga gtggtgttcg ggcggtgcgc gcaagatcca
     ttatgttaaa cgggcgagtt tacatctcaa aaccgcccgc ttaacaccat cagaaatcct
     cagcgcgatt ttaagcacca accccccccc gtaacaccca aatccatact gaaagtggct
     ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ctcccgacaa
     cacagaccat tccgtggcaa agcaaaagtt cagaatcacc aactggtcca cctacaacaa
     agctctcatc aaccgtggct ccctcacttt ctggctggat gatgaggcga ttcaggcctg
     gtatgagtcg gcaacacctt catcacgagg aaggccccag cgctattctg atctcgccat
     caccaccgtt ctggtgatta aacgcgtatt ccggctgacc ctgcgggctg cgcagggttt
     tattgattcc atttttgccc tgatgaacgt tccgttgcgc tgcccggatt acaccagtgt
     cagtaagcgg gcaaagtcgg ttaatgtcag tttcaaaacg tccacccggg gtgaaatcgc
     acacctggtg attgattcca ccgggctgaa ggtctttggt gaaggcgaat ggaaagtcag
     aaagcacggc aaagagcgcc gtcgtatctg gcgaaagttg catcttgctg ttgacagcaa
     cacacatgaa gttgtctgtg cagacctgtc gctgaataac gtcacggact cagaagcctt
     cccgggcctt atccggcaga ctcacagaaa aatcagggca gccgcggcag acggggctta
     cgatacccgg ctctgtcacg atgaactgcg ccgcaaaaaa atcagcgcgc ttattcctcc
     ccgaaaaggt gcgggttact ggcccggtga atatgcagac cgtaaccgtg cagtggctaa
     tcagcgaatg accgggagta atgcgcggtg gaaatggaca acagattaca accgtcgctc
     gatagcggaa acggcgatgt accgggtaaa acagctgttc gggggttcac tgacgctgcg
     tgactacgat ggtcaggttg cggaggctat ggccctggta cgagcgctga acaaaatgac
     gaaagcaggt atgcctgaaa gcgtgcgtat tgcctgaaaa cacaacccgc tacgggggag
     acttacccga aatctgattt attcaacaaa gccgggtgtg gtgaactaca aagcagaccc
     gttgaggtta tcagttcgat gcacaatcag cagcgcataa aatatgcaca agaacaggag
     cacccttcgc attaagctgt ggtggtaaca agtagtgccg ggctaccatc agcgagcatg
     atgcgctccc acagcattcg ccttggcagt atggaagttc ctcgctccag ttcgggccgg
     tatccacctc gagtgacccc agccgcccct catgccaacc gagcccatta agcgcagagt
     cggccgcaac tatctcgggg acgcataaac acgcagcgat ttaccaagga gttcggctag
     gtcaatccgc aattcccttt gcctggccgg agatgccgcg agcaaacaat gtgtccggtc
     gctaccagat ggactttgtg ggcggaggat tgcaccagac gcttcagcgc agcgtcgtac
     gatgaatcgg aaggcccggt aagcaattga agaaccgctg accacctccc atccgacaat
     gcaagggtgc cccgctccca gcgcgacacg gttgcctgat ccacacccat taattcagct
     aggtgacttt gcttcatatg acgaagcaac cgcgccctcc gcacctgccg ccccctgtca
     ttcgtcaaca ttcttcagca cctcaatgtc gttcgtagtg agcctcattt tttcaagacc
     gccgatgatg agagccaggc ctcgctcgaa tgccgcgtcc ggaccgcctt cgtagacgat
     tttcatcgcg ctctgtaggc gcgccggcat cgtagacgct gaggtggtca actgatcttc
     gccccgctcc tcggcgtctg cctcgctagc ttgctgctca agaacagcgc cgacggtgaa
     gtagctgatt gccatcaacg cataggtcgc gtcacctgcc gaaaagccag catcgcaaag
     gaagcgaagc tgcgcgtcgg ctttttccat ctgcggcgcg gctggccgcg tcccggcatg
     aatacgcgcg ccatcgcgat aagcgagcaa cgcccgtcga aaactgcatg cattgccctt
     caggaacgaa cgccagtcgt cgtcatccct tggcgtcgaa tgcgtgtgat ttatcgtcag
     catggcttcg gcaagtgcgt cgagcaacgc acgcttgttc ttgaaatgcc agtagagcgc
     tggctgttgc accccgaggc gctcagccag tcggcgcgtc gttagacctt ccatgcccac
     gtcgttaagc agttcgagcg cggttcggat cacggcctcg cgttggagct tgttcattcg
     cgaa
//