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ID   PSE280     preliminary; circular DNA; SYN; 3893 BP.
XX
AC   IG1115;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pSE280 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRSETA from pET-3 & pBluescript series
RC   pRSETB from pET-3 & pBluescript series
RC   pRSETC from pET-3 & pBluescript series
RC   pSE280 from pKK233-2
RC   pSE380 from pTrc99A
RC   pSE420 from pSE380
RC   pTRCHISA from pSE420
RC   pTRCHISB from pSE420
RC   pTRCHISC from pSE420
RA   Abdel-Malek H.;
RT   ;
RL   The Digest 4:1-3(1991).
XX
RN   [2]
RC   pKK421-1 from pKK233-2
RC   pSLD7, pSLE1, pSLF1, pSLJ10 from pBluescript KS+
RC   pSL180 from pUC18 & linker
RC   pSL189 from pUC19 & linker
RC   pSL190 from pSL189
RC   pSL1180 from pUC118 & linker
RC   pSL1190 from pUC119 & linker
RC   pSL300 from pSLJ10 & linker
RC   pSL250 from pSL300
RC   pSL260 from pSL250
RC   pSL270 from pSL250
RC   pSL251 from pSL250
RC   pSL261 from pSL260
RC   pSL271 from pSL270
RC   pSL301 from pSL300
RC   pSE1200 from pUC120 & linker
RC   pSE1201 from pUC120 & linker
RC   pSE1202 from pUC120 & linker
RC   pSE219 from pKK233-2
RC   pSE220 from pSE219
RC   pSE280 from pKK421-1
RC   pSE380 from pTrc99A
RA   Brosius J.;
RT   "Superpolylinkers in cloning and expression vectors";
RL   DNA 8:759-777(1989).
XX
CC   NM (pSE280)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pKK233-2)
CC   BR (pSE380)(pSE420)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK233-2 remove EcoRI-AvaI 1055bp
FT                   \ 4592..4593..1054, tet gene/3538bp
FT                   fill in:fill in
FT                   -> pKK421-1 3538bp
FT                   1. pKK421-1 3538bp
FT                   2. SL2 superlinker 355bp
FT                   -> pSE280 3893bp"
FT   promoter        0..0
FT                   /note="PRO E. coli trc (trp & lac)"
FT   RBS             0..0
FT                   /note="RBS"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS SL2 superlinker"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   terminator      0..0
FT                   /note="TER"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 3893 BP; 949 A; 964 C; 1023 G; 957 T; 0 other;
     aattctcatg tttgacagct tatcatcgac tgcacggtgc accaatgctt ctggcgtcag
     gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc
     gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg
     caaatattct gaaatgagct gttgacaatt aatcatccgg ctcgtataat gtgtggaatt
     gtgagcggat aacaatttca cacaggaaac agaccatggc tggtgaccac gtcgtggaat
     gccttcgaat tcagcacctg cacatgggac gtcgacctga ggtaattata acccgggccc
     tatatatgga tccaattgca atgatcatca tgacagatct gcgcgcgatc gatatcagcg
     ctttaaattt gcgcatgcta gctatagttc tagaggtacc ggttgttaac gttagccggc
     tacgtatact ccggaatatt aataggccta ggatgcatat ggcggccgcc tgcagctggc
     gccatcgata cgcgtacgtc gcgaccgcgg acatgtacag agctcgagaa gtactagtgg
     ccacgtgggc cgtgcacctt aagcttggct gttttggcgg atgagagaag attttcagcc
     tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
     aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
     acggatggcc tttttgcgtt tctacaaact ctttttgttt atttttctaa atacattcaa
     atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta
     aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag
     ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag
     ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg
     ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca
     tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca
     gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca
     gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt
     ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt
     cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg
     ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat
     gcgcacccgt ggccaggacc caacgctgcc cgaaattctc atgtttgaca gct
//