back Return to this vector's summary.
ID   PSE420     preliminary; circular DNA; SYN; 4613 BP.
XX
AC   IG1117;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pSE420 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRSETA from pET-3 & pBluescript series
RC   pRSETB from pET-3 & pBluescript series
RC   pRSETC from pET-3 & pBluescript series
RC   pSE280 from pKK233-2
RC   pSE380 from pTrc99A
RC   pSE420 from pSE380
RC   pTRCHISA from pSE420
RC   pTRCHISB from pSE420
RC   pTRCHISC from pSE420
RA   Abdel-Malek H.;
RT   ;
RL   The Digest 4:1-3(1991).
XX
RN   [2]
RC   pKK421-1 from pKK233-2
RC   pSLD7, pSLE1, pSLF1, pSLJ10 from pBluescript KS+
RC   pSL180 from pUC18 & linker
RC   pSL189 from pUC19 & linker
RC   pSL190 from pSL189
RC   pSL1180 from pUC118 & linker
RC   pSL1190 from pUC119 & linker
RC   pSL300 from pSLJ10 & linker
RC   pSL250 from pSL300
RC   pSL260 from pSL250
RC   pSL270 from pSL250
RC   pSL251 from pSL250
RC   pSL261 from pSL260
RC   pSL271 from pSL270
RC   pSL301 from pSL300
RC   pSE1200 from pUC120 & linker
RC   pSE1201 from pUC120 & linker
RC   pSE1202 from pUC120 & linker
RC   pSE219 from pKK233-2
RC   pSE220 from pSE219
RC   pSE280 from pKK421-1
RC   pSE380 from pTrc99A
RA   Brosius J.;
RT   "Superpolylinkers in cloning and expression vectors";
RL   DNA 8:759-777(1989).
XX
CC   NM (pSE420)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pSE380)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSE380 4476bp
FT                   -> pSE420 4613bp"
FT   promoter        0..0
FT                   /note="PRO E. coli trc (trp & lac)"
FT   promoter        0..0
FT                   /note="PRO E. coli lacO gene"
FT   promoter        0..0
FT                   /note="PRO E. coli antitermination"
FT   RBS             0..0
FT                   /note="RBS E. coli g10 gene"
FT   misc_feature    0..0
FT                   /note="E. coli mini-cistron"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS SL2 superlinker"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   terminator      0..0
FT                   /note="TER"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lacIq gene"
XX
SQ   Sequence 4613 BP; 1132 A; 1154 C; 1225 G; 1102 T; 0 other;
     gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
     ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
     gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
     tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
     taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
     caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
     aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatggctgg
     tgaccacgtc gtggaatgcc ttcgaattca gcacctgcac atgggacgtc gacctgaggt
     aattataacc cgggccctat atatggatcc aattgcaatg atcatcatga cagatctgcg
     cgcgatcgat atcagcgctt taaatttgcg catgctagct atagttctag aggtaccggt
     tgttaacgtt agccggctac gtatactccg gaatattaat aggcctagga tgcatatggc
     ggccgcctgc agctggcgcc atcgatacgc gtacgtcgcg accgcggaca tgtacagagc
     tcgagaagta ctagtggcca cgtgggccgt gcaccttaag cttggctgtt ttggcggatg
     agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
     gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
     gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca
     ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
     agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta
     agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt tttgtttatt
     tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     gcgaggcagc agatcaattc gcgcgcgaag gcgaagcggc atgcatttac gttgacacca
     tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca
     gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
     atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
     aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
     cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
     cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
     cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
     aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
     aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
     acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
     tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
     tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
     ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
     aagacagctc atgttatatc ccgccgtcaa ccaccatcaa acaggatttt cgcctgctgg
     ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc gcgaattgat ctg
//