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ID   PSFF       preliminary; circular DNA; SYN; 8742 BP.
XX
AC   Z22761;
XX
DT   19-MAY-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mammal retrovirus vector pSFF - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-8742
RC   pSFF from pL2-6K
RA   Perryman S., Nishio J., Chesebro B.;
RT   "Complete nucleotide sequence of the retroviral expression vector
RT   pSFF";
RL   Nucleic Acids Res. 22:0-0(1994).
XX
RN   [2]
RC   plasmid from pL2-6K
RC   pSFF from plasmid & linker
RC   psi2/SVSF from pSVSF
RA   Bestwick R.K., Kozak S.L., Kabat D.;
RT   "Overcoming interference to retroviral superinfection results in
RT   amplified expression and transmission of cloned genes";
RL   Proc. Natl. Acad. Sci. U.S.A. 85:5404-5408(1988).
XX
RN   [3]
RP   1-8742
RC   pSFF
RA   Perryman S.;
RT   ;
RL   Submitted (13-MAY-1993) on tape to the EMBL Data Library by:
RL   Perryman S., Rocky Mountain Laboratories 903 South 4th St.,
RL   Hamilton Montana USA.
XX
RN   [4]
RC   from pBR322-F-SFFV & pSP65, SFFV env gene/LTR
RC   from pBR322-F-SFFV & pSP64, SFFV gag gene/pol gene/LTR
RC   SFFV Sm1, SFFV Sm2, SFFV R1, SFFV Pvu, SFFV Bal from SFFV
RC   SFFV Pst, SFFV XB from SFFV
RC   [pL2-6K from pSP64 & Friend SFFV]
RA   Li J.P., Bestwick R.K., Spiro C.S., Kabat D.;
RT   "The membrane glycoprotein of Friend spleen focus-forming virus:
RT   evidence that the cell surface component is required for
RT   pathogenesis and that it binds to a receptor";
RL   J. Virol. 61:2782-2792(1987).
XX
RN   [5]
RC   pSVSF from pL2-6K & SV40
RA   Spiro C.S., Gliniak B., Kabat D.;
RT   "A tagged helper-free Friend virus causes clonal erythroblast
RT   immortality by specific proviral integration in the cellular
RT   genome";
RL   J. Virol. 62:4129-4135(1988).
XX
RN   [6]
RC   pBR322-F-SFFV from pBR322 & F-SFFV
RA   Linemeyer D., Ruscetti S., Menke J., Scolnick E.;
RT   "Recovery of biologically active spleen focus-forming virus from
RT   molecularly cloned spleen focus-forming virus-pBR322 circular DNA
RT   by cotransfection with infectious type C retroviral DNA";
RL   J. Virol. 35:710-721(1980).
XX
RN   [7]
RC   pMSVneo from MSV & neo gene
RA   Cone R.D., Mulligan R.C.;
RT   "High-efficiency gene transfer into mammalian cells: generation of
RT   helper-free recombinant retrovirus with braod mammalian host range";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:6349-6353(1984).
XX
CC   NM (pSFF)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(mammal)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA (pSP64)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSP64 remove PvuII-HindIII 225bp 8..233,
FT                   \ MCS/2774bp
FT                   2. Friend SFFV PvuII-HindIII, LTR/gag/pol
FT                   -> pL2-6K
FT                   1. pL2-6K remove EcoRI-BamHI, pol gene
FT                   blunt end:blunt end
FT                   -> plasmid
FT                   1. plasmid remove BamHI-EcoRI, env gene
FT                   blunt end:blunt end
FT                   XhoI linker 6bp ctcgag:XhoI linker 6bp ctcgag
FT                   -> pSFF 8742bp [BamHI, EcoRI]"
FT   LTR             1..558
FT                   /note="RPT Long Terminal Repeat; repeated
FT                   from bases 4767 to 5324; EXPERIMENTAL [1] [2]"
FT   primer_bind     559..576
FT                   /note="PRI Primer Binding Site; repeated
FT                   from bases 5325 to 5342; EXPERIMENTAL [1] [2]"
FT   misc_feature    577..1032
FT                   /note="5' untranslated region; repeat of
FT                   bases 577 to 771 after the second LTR at bases 5343
FT                   to 5537, due to the method used to
FT                   create this clone [1] [2]"
FT   CDS             1033..1789
FT                   /note="GEN E. coli RNA packaging, viral protease,
FT                   ribonucleoprotein, gag protein;
FT                   EXPERIMENTAL; group antigen [1] [2]"
FT   misc_feature    3939..3958
FT                   /note="MCS BamHI-XhoI-EcoRI linker; located within
FT                   the defective env gene of the retrovirus [1] [2]"
FT   repeat_region   3982..4766
FT                   /note="RPT part of the defective envelope
FT                   gene env, repeated between bases 7958 and 8742,
FT                   due to the method used to create this clone [1] [2]"
FT   LTR             4767..5324
FT                   /note="RPT Long Terminal Repeat; repeat of the
FT                   region between bases 1 and 558; EXPERIMENTAL [1] [2]"
FT   repeat_region   5325..5342
FT                   /note="RPT repeat of the region between bases
FT                   559 and 576, the primer binding site for the
FT                   retrovirus; repeated due to the method which was
FT                   used to create this clone [1] [2]"
FT   repeat_region   5343..5537
FT                   /note="RPT repeat of the 5' untranslated region
FT                   located between bases 577 and 771; occurred
FT                   due to the method used to create this clone [1] [2]"
FT   misc_feature    5538..7957
FT                   /note="from pSP64; vector sequence between bases 233
FT                   and 2652 are given in reverse orientation [1] [2]"
FT   repeat_region   7958..8742
FT                   /note="RPT repeat of the defective envelope gene env
FT                   sequences between bases 3982 and 4766 [1] [2]"
XX
SQ   Sequence 8742 BP; 2127 A; 2459 C; 2153 G; 2003 T; 0 other;
     aatgaaagac cccaccaaat tgcttagcct gatgccgctg taacgccatt ttgcaaggca
     tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     gtcctccgac agactgagtc gcccgggtac ccgtgtatcc aataaatcct cttgctgttg
     catccgactc gtggtctcgc tgttccttgg gagggtctcc tcagagtgat tgactacccg
     tctcgggggt ctttcatttg ggggctcgtc cgggatctgg agacccctgc ccagggacca
     ccgacccacc accgggaggt aagctggcca gcaattgttc tgtgtctgtc cattgtcccg
     tgtccttgat tgtttttata cgcctgcgtc ggtgctagtt ggccaactag atctgtatct
     ggcggacccg tggtggaact gacgagttcg agacacccgg ccgcaaccct gggagacgtc
     ccagggactt cgggggccat gtttgtggcc cggccagagt ccaaccatcc cgatcgtttt
     ggtctcttcg gtgcaccccc cttagaggag gggtatgtgg ttctggtagg ggacggaaga
     ctaaaacggt ttccgccccc gtctgagttt ttgctttcgg tttggaaccg aagccgcgcc
     gcgcgtcttg tctgctgcag catcgttcta tgttgtcttt gtttgactgt ttttctgtat
     ttgtctgaaa acatgggcca gactgttacc acccccttaa gtttgacttt agatcactgg
     aaggatgtcg aacggacagc ccacaacctg tcggtagagg ttggaaaaag gcgctgggtt
     acattctgct ctgcagaatg gccagccttc aacgtcggat ggccacgaga cggcactttt
     aacccagaca ttattacaca ggttaagatc aaggtcttct cacctggccc acatggacat
     ccggatcagg tcccctacat cgtgacctgg gaagctatag cagtagaccc ccctccctgg
     gtcagaccct tcatgcaccc taaacctccc ctccttcttt ccccttcagc cccctctctc
     ccacctgaac ccccactctc gaccccgcct cagtcctccc tctatccggc tctcacttct
     cctttaaaca ctaaacctag gcctcaagtc cttcctgata gcggaggacc actcattgat
     ccactcacgg aggaccctcc gccttaccgg gacccagggc caccctctcc tgacgggaac
     ggcgatagcg gagaagtggc ccctacagaa ggggcccctg actcttcccc aatggtatcc
     cgcctgcggg gaagaagaga accccccgtg gcggattcta ctacctctca ggcgttcccc
     cttcgcctgg gagggaatgg acagtttcaa tactggccat tttcctcctc tgacctctat
     aactggaaaa ataacaatcc ctctttctcc gaggacccag ctaaattgac agctttgatc
     gagtccgttc tccttactca tcagcccact tgggatgact gccaacagct attagggacc
     ctgctgacgg gagaagaaaa acagcgagtg ctcctagagg cccgaaaggc ggttcgaggg
     gaggacggac gcccaactca gctgcccaat gaaattaatg atgcttttcc cttggaacgt
     cccgactggg actacaacac ccaacgaggt aggaaccacc tagtccacta tcgccagttg
     ctcctagcgg gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaagaggg
     ataacccaat gacctaatga gtctccctca gcctttttag agagactcaa ggaggcctac
     cgcagataca ctccttatga ccctgaggac ccagggcaag aaaccaatgt gtccatgtca
     ttcatctggc agtccgcccc ggatatcggg cgaaagttag agcggttaga agatttgaag
     agtaagacct taggagactt agtgagggaa gctgaaaaga tctattagga gagaaacaga
     ggagaaggaa gaacgccgta gggcagagga tgagcagaga gagaaggaga gggaccgcag
     aagacataga gaaatgagta agttgccggc tactgtcgtt agcgggcaga gacaggatag
     acagggagga gagcgaagga ggccccaact cgaccatgac cagtgtgcct actgcaaaga
     cccaccagtg gtacactgtg cttgatttga aagatgtttt tttttccgcc tgagactcca
     ccccaccagt cagtctctct tcgccttgag tggagagatc cagagatggg aatctcagga
     caattaatct ggaccagact cccacagggt ttcaaaaaca gtcccaccct gtttgatgaa
     gccctgcaca gagacctcgt agacttccgg atcaattcct agggacggca ggcttctgtc
     gcctctagat ccctgggttt gcagaaatgg cagccccctt gtaccctctc accaaaacgg
     ggactttgtt taagtggggc ccagaccagc aaaaggccta ccaagagatc aagcaggcta
     tcttaactgc ccctgccctg ggatcgccag acttgactaa gcccttcaaa aactagaccc
     agtggcagct gggtggcccc cttgcctacg gatggtaaca gccatcgccg ttctgaccaa
     agacgctggc aagctcacca tgggacagcc actagtcatt ctggcccccc atgcagtaga
     ggcactagtt aagcaacccc ctgatcgctg gctctccaac gcccgaatga cccactacca
     ggctctgctt ctggacacgg accgagtcca gttcggacca atagtggccc taaacccagc
     tacgctgctc cctctatctg aggaagggct gcagcatgac tgccttgaca tcttggctga
     agcccacgga actagaccag atcttacgga ccagcctccc ccagacgctg accacacccg
     gtacacagat gggagcagct tcctgcaaga ggggcagcgc aaggccggag cagcaataac
     caccgagacc gagaagtttt cgtagacact ttttctggat agatagaagc tttcccaacc
     aagaaagaaa ctgccaaagt tgtaaccaag aagctactaa aagaaatctt ccccagattc
     ggcatgccac aggtattggg aaccaacaat gggcctgccc tcgtctccaa ggtaagtcag
     acagtagccg atttattggg ggttgattgg aaactacatt gtgcttacag accccagagt
     tcaggtcagg tagaaagaat gaataggaca atcaaggaga ctttaactaa attgacgctt
     gcaactggct ctagggactg ggtgctcctg cttcccccgg ccctgtatca agcccgcaac
     acgccgggcc cccatggtct caccccatat gacatggcaa aggtcactca taacccctct
     ctccaagctc atttacaggc actctacttg gtccagcaca aagtctggag accgttggcg
     gcagcttacc aagaacaact agaccggccg gtagtacctc accctttccg agtcggtgac
     acagtgtggg tccgcagaca ccagcggcat cgctgcgtgg atcccctcga gggaattcac
     tgacgcgggt agaaaggcca gctgggatgc ccccaaagta tggggactga gactgtaccg
     atccacaggg accgacccgg tgacccggtt ctctttgacc cgccaggtcc tcgatatagg
     gccccgcgtt cccattgggt ctaatcccgt gaccactgac cagttacccc tctcccgacc
     cgtgcagacc atgctcccca ggcctcttca gcctcctcct ccaggcgcag cctctatagt
     ccccgagact gccccacctc ctcaacaacc tggggcggga gacaggctgc taaacctggt
     agatggggcc taccaagctc tcaacctcac caaccctgat aaaattcaag agtgctggtt
     atgcctagtg tctggacccc cctattacga gggggttgtg gtcctaggca cttattttaa
     tcataccatt gccctaaaag aaaaatgttg tttctatgct gaccatacag gcctagtaag
     agatagcatg gccaaattaa gaaagagact cactcagaga caaaaattat ttgagtcgag
     ccgaggatgg ttcgaaggat cgtctaacag atccccctgg tttaccacgt tgatatccgc
     catcatgggg tctctcatta tactcctact cctactaatt ctgcttattt ggaccctgta
     ttcttaatca attagttcaa tttgttaaag acaggatctc agtagtccag gctttagtcc
     tgactcaaca ataccaccag ctaaaaccac tagaatacga gccacaataa ataaaagatt
     ttatttagtt tccagaaaaa ggggggaatg aaagacccca ccaaattgct tagcctgatg
     ccgctgtaac gccattttgc aaggcatgga aaaataccaa accaagaata gagaagttca
     gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat ctgcggtgag
     cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg gccccggccc
     gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc ttaagaccca
     tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat ttgaattaac
     caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc tataaaagag
     ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc gggtacccgt
     gtatccaata aatcctcttg ctgttgcatc cgactcgtgg tctcgctgtt ccttgggagg
     gtctcctcag agtgattgac tacccgtctc gggggtcttt catttggggg ctcgtccggg
     atctggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa
     ttgttctgtg tctgtccatt gtcccgtgtc cttgattgtt tttatacgcc tgcgtcggtg
     ctagttggcc aactagatct gtatctggcg gacccgtggt ggaactgacg agttcgagac
     acccggccgc aaccctgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     aagggagagc gtcgatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg ggatgccccc aaagtatggg
     gactgagact gtaccgatcc acagggaccg acccggtgac ccggttctct ttgacccgcc
     aggtcctcga tatagggccc cgcgttccca ttgggtctaa tcccgtgacc actgaccagt
     tacccctctc ccgacccgtg cagaccatgc tccccaggcc tcttcagcct cctcctccag
     gcgcagcctc tatagtcccc gagactgccc cacctcctca acaacctggg gcgggagaca
     ggctgctaaa cctggtagat ggggcctacc aagctctcaa cctcaccaac cctgataaaa
     ttcaagagtg ctggttatgc ctagtgtctg gaccccccta ttacgagggg gttgtggtcc
     taggcactta ttttaatcat accattgccc taaaagaaaa atgttgtttc tatgctgacc
     atacaggcct agtaagagat agcatggcca aattaagaaa gagactcact cagagacaaa
     aattatttga gtcgagccga ggatggttcg aaggatcgtc taacagatcc ccctggttta
     ccacgttgat atccgccatc atggggtctc tcattatact cctactccta ctaattctgc
     ttatttggac cctgtattct taatcaatta gttcaatttg ttaaagacag gatctcagta
     gtccaggctt tagtcctgac tcaacaatac caccagctaa aaccactaga atacgagcca
     caataaataa aagattttat ttagtttcca gaaaaagggg gg
//