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ID PSFF preliminary; circular DNA; SYN; 8742 BP.
XX
AC Z22761;
XX
DT 19-MAY-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Mammal retrovirus vector pSFF - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-8742
RC pSFF from pL2-6K
RA Perryman S., Nishio J., Chesebro B.;
RT "Complete nucleotide sequence of the retroviral expression vector
RT pSFF";
RL Nucleic Acids Res. 22:0-0(1994).
XX
RN [2]
RC plasmid from pL2-6K
RC pSFF from plasmid & linker
RC psi2/SVSF from pSVSF
RA Bestwick R.K., Kozak S.L., Kabat D.;
RT "Overcoming interference to retroviral superinfection results in
RT amplified expression and transmission of cloned genes";
RL Proc. Natl. Acad. Sci. U.S.A. 85:5404-5408(1988).
XX
RN [3]
RP 1-8742
RC pSFF
RA Perryman S.;
RT ;
RL Submitted (13-MAY-1993) on tape to the EMBL Data Library by:
RL Perryman S., Rocky Mountain Laboratories 903 South 4th St.,
RL Hamilton Montana USA.
XX
RN [4]
RC from pBR322-F-SFFV & pSP65, SFFV env gene/LTR
RC from pBR322-F-SFFV & pSP64, SFFV gag gene/pol gene/LTR
RC SFFV Sm1, SFFV Sm2, SFFV R1, SFFV Pvu, SFFV Bal from SFFV
RC SFFV Pst, SFFV XB from SFFV
RC [pL2-6K from pSP64 & Friend SFFV]
RA Li J.P., Bestwick R.K., Spiro C.S., Kabat D.;
RT "The membrane glycoprotein of Friend spleen focus-forming virus:
RT evidence that the cell surface component is required for
RT pathogenesis and that it binds to a receptor";
RL J. Virol. 61:2782-2792(1987).
XX
RN [5]
RC pSVSF from pL2-6K & SV40
RA Spiro C.S., Gliniak B., Kabat D.;
RT "A tagged helper-free Friend virus causes clonal erythroblast
RT immortality by specific proviral integration in the cellular
RT genome";
RL J. Virol. 62:4129-4135(1988).
XX
RN [6]
RC pBR322-F-SFFV from pBR322 & F-SFFV
RA Linemeyer D., Ruscetti S., Menke J., Scolnick E.;
RT "Recovery of biologically active spleen focus-forming virus from
RT molecularly cloned spleen focus-forming virus-pBR322 circular DNA
RT by cotransfection with infectious type C retroviral DNA";
RL J. Virol. 35:710-721(1980).
XX
RN [7]
RC pMSVneo from MSV & neo gene
RA Cone R.D., Mulligan R.C.;
RT "High-efficiency gene transfer into mammalian cells: generation of
RT helper-free recombinant retrovirus with braod mammalian host range";
RL Proc. Natl. Acad. Sci. U.S.A. 81:6349-6353(1984).
XX
CC NM (pSFF)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)(mammal)
CC CP ()
CC FN (cloning)(expression)
CC SE ()
CC PA (pSP64)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pSP64 remove PvuII-HindIII 225bp 8..233,
FT \ MCS/2774bp
FT 2. Friend SFFV PvuII-HindIII, LTR/gag/pol
FT -> pL2-6K
FT 1. pL2-6K remove EcoRI-BamHI, pol gene
FT blunt end:blunt end
FT -> plasmid
FT 1. plasmid remove BamHI-EcoRI, env gene
FT blunt end:blunt end
FT XhoI linker 6bp ctcgag:XhoI linker 6bp ctcgag
FT -> pSFF 8742bp [BamHI, EcoRI]"
FT LTR 1..558
FT /note="RPT Long Terminal Repeat; repeated
FT from bases 4767 to 5324; EXPERIMENTAL [1] [2]"
FT primer_bind 559..576
FT /note="PRI Primer Binding Site; repeated
FT from bases 5325 to 5342; EXPERIMENTAL [1] [2]"
FT misc_feature 577..1032
FT /note="5' untranslated region; repeat of
FT bases 577 to 771 after the second LTR at bases 5343
FT to 5537, due to the method used to
FT create this clone [1] [2]"
FT CDS 1033..1789
FT /note="GEN E. coli RNA packaging, viral protease,
FT ribonucleoprotein, gag protein;
FT EXPERIMENTAL; group antigen [1] [2]"
FT misc_feature 3939..3958
FT /note="MCS BamHI-XhoI-EcoRI linker; located within
FT the defective env gene of the retrovirus [1] [2]"
FT repeat_region 3982..4766
FT /note="RPT part of the defective envelope
FT gene env, repeated between bases 7958 and 8742,
FT due to the method used to create this clone [1] [2]"
FT LTR 4767..5324
FT /note="RPT Long Terminal Repeat; repeat of the
FT region between bases 1 and 558; EXPERIMENTAL [1] [2]"
FT repeat_region 5325..5342
FT /note="RPT repeat of the region between bases
FT 559 and 576, the primer binding site for the
FT retrovirus; repeated due to the method which was
FT used to create this clone [1] [2]"
FT repeat_region 5343..5537
FT /note="RPT repeat of the 5' untranslated region
FT located between bases 577 and 771; occurred
FT due to the method used to create this clone [1] [2]"
FT misc_feature 5538..7957
FT /note="from pSP64; vector sequence between bases 233
FT and 2652 are given in reverse orientation [1] [2]"
FT repeat_region 7958..8742
FT /note="RPT repeat of the defective envelope gene env
FT sequences between bases 3982 and 4766 [1] [2]"
XX
SQ Sequence 8742 BP; 2127 A; 2459 C; 2153 G; 2003 T; 0 other;
aatgaaagac cccaccaaat tgcttagcct gatgccgctg taacgccatt ttgcaaggca
tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
gtcctccgac agactgagtc gcccgggtac ccgtgtatcc aataaatcct cttgctgttg
catccgactc gtggtctcgc tgttccttgg gagggtctcc tcagagtgat tgactacccg
tctcgggggt ctttcatttg ggggctcgtc cgggatctgg agacccctgc ccagggacca
ccgacccacc accgggaggt aagctggcca gcaattgttc tgtgtctgtc cattgtcccg
tgtccttgat tgtttttata cgcctgcgtc ggtgctagtt ggccaactag atctgtatct
ggcggacccg tggtggaact gacgagttcg agacacccgg ccgcaaccct gggagacgtc
ccagggactt cgggggccat gtttgtggcc cggccagagt ccaaccatcc cgatcgtttt
ggtctcttcg gtgcaccccc cttagaggag gggtatgtgg ttctggtagg ggacggaaga
ctaaaacggt ttccgccccc gtctgagttt ttgctttcgg tttggaaccg aagccgcgcc
gcgcgtcttg tctgctgcag catcgttcta tgttgtcttt gtttgactgt ttttctgtat
ttgtctgaaa acatgggcca gactgttacc acccccttaa gtttgacttt agatcactgg
aaggatgtcg aacggacagc ccacaacctg tcggtagagg ttggaaaaag gcgctgggtt
acattctgct ctgcagaatg gccagccttc aacgtcggat ggccacgaga cggcactttt
aacccagaca ttattacaca ggttaagatc aaggtcttct cacctggccc acatggacat
ccggatcagg tcccctacat cgtgacctgg gaagctatag cagtagaccc ccctccctgg
gtcagaccct tcatgcaccc taaacctccc ctccttcttt ccccttcagc cccctctctc
ccacctgaac ccccactctc gaccccgcct cagtcctccc tctatccggc tctcacttct
cctttaaaca ctaaacctag gcctcaagtc cttcctgata gcggaggacc actcattgat
ccactcacgg aggaccctcc gccttaccgg gacccagggc caccctctcc tgacgggaac
ggcgatagcg gagaagtggc ccctacagaa ggggcccctg actcttcccc aatggtatcc
cgcctgcggg gaagaagaga accccccgtg gcggattcta ctacctctca ggcgttcccc
cttcgcctgg gagggaatgg acagtttcaa tactggccat tttcctcctc tgacctctat
aactggaaaa ataacaatcc ctctttctcc gaggacccag ctaaattgac agctttgatc
gagtccgttc tccttactca tcagcccact tgggatgact gccaacagct attagggacc
ctgctgacgg gagaagaaaa acagcgagtg ctcctagagg cccgaaaggc ggttcgaggg
gaggacggac gcccaactca gctgcccaat gaaattaatg atgcttttcc cttggaacgt
cccgactggg actacaacac ccaacgaggt aggaaccacc tagtccacta tcgccagttg
ctcctagcgg gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaagaggg
ataacccaat gacctaatga gtctccctca gcctttttag agagactcaa ggaggcctac
cgcagataca ctccttatga ccctgaggac ccagggcaag aaaccaatgt gtccatgtca
ttcatctggc agtccgcccc ggatatcggg cgaaagttag agcggttaga agatttgaag
agtaagacct taggagactt agtgagggaa gctgaaaaga tctattagga gagaaacaga
ggagaaggaa gaacgccgta gggcagagga tgagcagaga gagaaggaga gggaccgcag
aagacataga gaaatgagta agttgccggc tactgtcgtt agcgggcaga gacaggatag
acagggagga gagcgaagga ggccccaact cgaccatgac cagtgtgcct actgcaaaga
cccaccagtg gtacactgtg cttgatttga aagatgtttt tttttccgcc tgagactcca
ccccaccagt cagtctctct tcgccttgag tggagagatc cagagatggg aatctcagga
caattaatct ggaccagact cccacagggt ttcaaaaaca gtcccaccct gtttgatgaa
gccctgcaca gagacctcgt agacttccgg atcaattcct agggacggca ggcttctgtc
gcctctagat ccctgggttt gcagaaatgg cagccccctt gtaccctctc accaaaacgg
ggactttgtt taagtggggc ccagaccagc aaaaggccta ccaagagatc aagcaggcta
tcttaactgc ccctgccctg ggatcgccag acttgactaa gcccttcaaa aactagaccc
agtggcagct gggtggcccc cttgcctacg gatggtaaca gccatcgccg ttctgaccaa
agacgctggc aagctcacca tgggacagcc actagtcatt ctggcccccc atgcagtaga
ggcactagtt aagcaacccc ctgatcgctg gctctccaac gcccgaatga cccactacca
ggctctgctt ctggacacgg accgagtcca gttcggacca atagtggccc taaacccagc
tacgctgctc cctctatctg aggaagggct gcagcatgac tgccttgaca tcttggctga
agcccacgga actagaccag atcttacgga ccagcctccc ccagacgctg accacacccg
gtacacagat gggagcagct tcctgcaaga ggggcagcgc aaggccggag cagcaataac
caccgagacc gagaagtttt cgtagacact ttttctggat agatagaagc tttcccaacc
aagaaagaaa ctgccaaagt tgtaaccaag aagctactaa aagaaatctt ccccagattc
ggcatgccac aggtattggg aaccaacaat gggcctgccc tcgtctccaa ggtaagtcag
acagtagccg atttattggg ggttgattgg aaactacatt gtgcttacag accccagagt
tcaggtcagg tagaaagaat gaataggaca atcaaggaga ctttaactaa attgacgctt
gcaactggct ctagggactg ggtgctcctg cttcccccgg ccctgtatca agcccgcaac
acgccgggcc cccatggtct caccccatat gacatggcaa aggtcactca taacccctct
ctccaagctc atttacaggc actctacttg gtccagcaca aagtctggag accgttggcg
gcagcttacc aagaacaact agaccggccg gtagtacctc accctttccg agtcggtgac
acagtgtggg tccgcagaca ccagcggcat cgctgcgtgg atcccctcga gggaattcac
tgacgcgggt agaaaggcca gctgggatgc ccccaaagta tggggactga gactgtaccg
atccacaggg accgacccgg tgacccggtt ctctttgacc cgccaggtcc tcgatatagg
gccccgcgtt cccattgggt ctaatcccgt gaccactgac cagttacccc tctcccgacc
cgtgcagacc atgctcccca ggcctcttca gcctcctcct ccaggcgcag cctctatagt
ccccgagact gccccacctc ctcaacaacc tggggcggga gacaggctgc taaacctggt
agatggggcc taccaagctc tcaacctcac caaccctgat aaaattcaag agtgctggtt
atgcctagtg tctggacccc cctattacga gggggttgtg gtcctaggca cttattttaa
tcataccatt gccctaaaag aaaaatgttg tttctatgct gaccatacag gcctagtaag
agatagcatg gccaaattaa gaaagagact cactcagaga caaaaattat ttgagtcgag
ccgaggatgg ttcgaaggat cgtctaacag atccccctgg tttaccacgt tgatatccgc
catcatgggg tctctcatta tactcctact cctactaatt ctgcttattt ggaccctgta
ttcttaatca attagttcaa tttgttaaag acaggatctc agtagtccag gctttagtcc
tgactcaaca ataccaccag ctaaaaccac tagaatacga gccacaataa ataaaagatt
ttatttagtt tccagaaaaa ggggggaatg aaagacccca ccaaattgct tagcctgatg
ccgctgtaac gccattttgc aaggcatgga aaaataccaa accaagaata gagaagttca
gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat ctgcggtgag
cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg gccccggccc
gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc ttaagaccca
tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat ttgaattaac
caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc tataaaagag
ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc gggtacccgt
gtatccaata aatcctcttg ctgttgcatc cgactcgtgg tctcgctgtt ccttgggagg
gtctcctcag agtgattgac tacccgtctc gggggtcttt catttggggg ctcgtccggg
atctggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa
ttgttctgtg tctgtccatt gtcccgtgtc cttgattgtt tttatacgcc tgcgtcggtg
ctagttggcc aactagatct gtatctggcg gacccgtggt ggaactgacg agttcgagac
acccggccgc aaccctgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
aagggagagc gtcgatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg ggatgccccc aaagtatggg
gactgagact gtaccgatcc acagggaccg acccggtgac ccggttctct ttgacccgcc
aggtcctcga tatagggccc cgcgttccca ttgggtctaa tcccgtgacc actgaccagt
tacccctctc ccgacccgtg cagaccatgc tccccaggcc tcttcagcct cctcctccag
gcgcagcctc tatagtcccc gagactgccc cacctcctca acaacctggg gcgggagaca
ggctgctaaa cctggtagat ggggcctacc aagctctcaa cctcaccaac cctgataaaa
ttcaagagtg ctggttatgc ctagtgtctg gaccccccta ttacgagggg gttgtggtcc
taggcactta ttttaatcat accattgccc taaaagaaaa atgttgtttc tatgctgacc
atacaggcct agtaagagat agcatggcca aattaagaaa gagactcact cagagacaaa
aattatttga gtcgagccga ggatggttcg aaggatcgtc taacagatcc ccctggttta
ccacgttgat atccgccatc atggggtctc tcattatact cctactccta ctaattctgc
ttatttggac cctgtattct taatcaatta gttcaatttg ttaaagacag gatctcagta
gtccaggctt tagtcctgac tcaacaatac caccagctaa aaccactaga atacgagcca
caataaataa aagattttat ttagtttcca gaaaaagggg gg
//