Return to this vector's summary.
ID PSG424 preliminary; circular DNA; SYN; 4978 BP.
XX
AC IG8003;
XX
DT 01-DEC-1994 (Rel. 10, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pSG424 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pSG424 from pECE
RA Sadowski I., Ptashne M.;
RT "A vector for expressing GAL4(1-147) fusions in mammalian cells";
RL Nucleic Acids Res. 17:7539-7539(1989).
XX
RN [2]
RC plasmid from pLSV40 & oligo
RC pECE from plasmid & linker
RC [pMT21 from pMT series]
RC pRB from pMT21 & hlR cDNA
RC pT from pRB & hlR cDNA
RC peT from pECE & pT
RC plasmid2 from peT & M13mp19
RC peYF1, peYF3 from plasmid2 & pECE
RC from peT & pECE
RA Ellis L., Clauser E., Morgan D.O., Edery M., Roth R.A., Rutter W.J.;
RT "Replacement of insulin receptor tyrosine residues 1162 and 1163
RT compromises insulin-stimulated kinase activity and uptake of
RT 2-deoxyglucose";
RL Cell 45:721-732(1986).
XX
RN [3]
RC pM1 from pSG424 & pSP72 & pMTL23
RC pM2, pM3 from pM1
RC plasmid from pMH76 & linker
RC pY1 from pM1 & plasmid
RC pY2 from pM2 & plasmid
RC pY3 from pM3 & plasmid
RC pM1VP16 from pCRF2 & pM1
RC pM2VP16 from pCRF1 & pM2
RC pM3VP16 from pCRF3 & pM3
RC pY1VP16 from pCRF2 & pM1
RC pY2VP16 from pCRF1 & pM2
RC pY3VP16 from pCRF3 & pM3
RA Sadowski I., Bell B., Broad P., Hollis M.;
RT "GAL4 fusion vectors for expression in yeast or mammalian cells";
RL Gene 118:137-141(1992).
XX
RN [4]
RC pDN3 from YEplac112 & GAL4 gene
RC YCpG4 from YCplac22 & GAL4 gene
RC from YIplac128 & GAL4 gene
RA Sadowski I., Niedbala D., Wood K., Ptashne M.;
RT "GAL4 is phosphorylated as a consequence of transcriptional
RT activation";
RL Proc. Natl. Acad. Sci. U.S.A. 88:10510-10514(1991).
XX
RN [5]
RC pKLC15 from GAL4 gene
RC pMA210 from GAL4 gene
RA Ma J., Ptashne M.;
RT "Deletion analysis of GAL4 defines two transcriptional activating
RT segments";
RL Cell 48:847-853(1987).
XX
RN [6]
RC pMH76 from pUC19 & ARS/CEN6 & TRP1 & GAL4 gene
RA Hollis M.;
RT ;
RL Unpublished (1991).
XX
RN [7]
RC pMA424 from GAL4 gene
RA Ma J., Ptashne M.;
RT "A new class of yeast transcriptional activators";
RL Cell 51:113-119(1987).
XX
RN [8]
RC pSV40 from pBR322 & SV40
RC pLSV40 from pSV40 & linker
RA Valenzuela P., Heberlein U., Mullenbach G.;
RT ;
RL Unpublished (1986).
XX
RN [9]
RC pMT1 from adenovirus major late promoter
RC pMT2 from pMT1
RC pMT3 from pMT2
RC pMT21 from pMT2
RA Kaufman R.J., Davies M.V., Pathak V.K., Hershey J.W.;
RT "The phosphorylation state of eucaryotic initiation factor 2
RT alters translational efficiency of specific mRNAs";
RL Mol. Cell. Biol. 9:946-958(1989).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC MCS. oligonucleotide linker.
CC NM (YCpGAL1)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pUC19)(pBM272)(YCplac111)(pCRF)(pBR322)(SV40)
CC BR (YCpGAL2)(YCpGAL3)
CC OF (pDBD)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 BamHI 4361bp 376..376
FT 2. SV40 BamHI 5243bp 2534..2534
FT -> pSV40 9604bp
FT 1. pSV40 HindIII 9604bp, SV40 5172..5172
FT Klenow
FT BclI 9608bp, SV40 2271..2271
FT 2. linker BglII-SmaI-XbaI 26bp
FT \ agatctcccgggtctagataagtaat
FT -> pLSV40 (OL/PL) 9634bp
FT 1. pLSV40 (OL/PL) remove KpnI-AatII 3987bp,
FT \ SV40 299..4286/pBR322 AatII 4291/5647bp
FT T4 DNA polymerase:T4 DNA polymerase
FT -> plasmid 6200bp
FT 1. plasmid remove NdeI-SalI 1645bp, pBR322 652..2297
FT \ 4600bp
FT \ SV40 NdeI 3811 4829 no SalI
FT Klenow:Klenow
FT 2. linker BglII-HindIII-SalI-KpnI-SmaI-EcoRI-SstI-XbaI
FT \ 39bp gatctaagcttgtcgacggtaccccggggaattcgagct
FT -> pECE 4600bp
FT 1. pECE remove HindIII-EcoRI 23bp,
FT \ linker MCS/4600bp
FT 2. yeast HindIII-EcoRI 424bp, GAL4(1-147) gene/#K01486
FT \ pGAD424 424bp 410..834 [544bp]
FT \ agctttgcaaagatggataaagcggaattaattcccgagcctccaaaaaa
FT \ gaagagaaaggtcgaattgggtaccgccgccaattttaatcaaagtggga
FT \ atattgctgatagctcattgtccttcactttcactaacagtagcaacggt
FT \ ccgaacctcataacaactcaaacaaattctcaagcgctttcacaaccaat
FT \ tgcctcctctaacgttcatgataacttcatgaataatgaaatcacggcta
FT \ gtaaaattgatgatggtaataattcaaaaccactgtcacctggttggacg
FT \ gaccaaactgcgtataacgcgtttggaatcactacagggatgtttaatac
FT \ cactacaatggatgatgtatataactatctattcgatgatgaagataccc
FT \ caccaaacccaaaaaaagagatcg
FT -> pSG424 4473bp"
FT - 1..1976
FT /note="SV40 295..2270 1976bp
FT BclI = T^GATCA
FT \ gatct..."
FT - 1977..2001
FT /note="gatctcccgggtctagataagtaat 25bp
FT \ ...aagtaat
FT BclI = T^GATCA"
FT - 2002..2264
FT /note="SV40 2271..2533 263bp
FT BamHI = G^GATCC"
FT - 2265..2544
FT /note="pBR322 376..655 280bp
FT SalI = G^TCGA C
FT \ gatcta..."
FT - 2545..2550
FT /note="gatcta 6bp
FT \ gatcta
FT HindIII = A^AGCTT"
FT - 2551..2974
FT /note="424bp
FT \ agctttgcaaagatggataaagcggaattaattcccgagcctccaaaaaa
FT \ gaagagaaaggtcgaattgggtaccgccgccaattttaatcaaagtggga
FT \ atattgctgatagctcattgtccttcactttcactaacagtagcaacggt
FT \ ccgaacctcataacaactcaaacaaattctcaagcgctttcacaaccaat
FT \ tgcctcctctaacgttcatgataacttcatgaataatgaaatcacggcta
FT \ gtaaaattgatgatggtaataattcaaaaccactgtcacctggttggacg
FT \ gaccaaactgcgtataacgcgtttggaatcactacagggatgtttaatac
FT \ cactacaatggatgatgtatataactatctattcgatgatgaagataccc
FT \ caccaaacccaaaaaaagagatcg
FT \ ...gatcg
FT EcoRI = G^AATTC
FT \ aattcgagct"
FT - 2975..2984
FT /note="aattcgagct 10bp
FT \ aattcgagct
FT NdeI = CA^TATG"
FT - 2985..4978
FT /note="pBR322 2297..4290 1994bp
FT AatII = GACGT^C
FT KpnI = Asp718I = G GTAC^C"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ Sequence 4978 BP; 1355 A; 1121 C; 1193 G; 1309 T; 0 other;
gtacctaacc aagttcctct ttcagaggtt atttcaggcc atggtgctgc gccggctgtc
acgccaggcc tccgttaagg ttcgtaggtc atggactgaa agtaaaaaaa cagctcaacg
cctttttgtg tttgttttag agcttttgct gcaattttgt gaaggggaag atactgttga
cgggaaacgc aaaaaaccag aaaggttaac tgaaaaacca gaaagttaac tggtaagttt
agtctttttg tcttttattt caggtccatg ggtgctgctt taacactgtt gggggaccta
attgctactg tgtctgaagc tgctgctgct actggatttt cagtagctga aattgctgct
ggagaggccg ctgctgcaat tgaagtgcaa cttgcatctg ttgctactgt tgaaggccta
acaacctctg aggcaattgc tgctataggc ctcactccac aggcctatgc tgtgatatct
ggggctcctg ctgctatagc tggatttgca gctttactgc aaactgtgac tggtgtgagc
gctgttgctc aagtggggta tagatttttt agtgactggg atcacaaagt ttctactgtt
ggtttatatc aacaaccagg aatggctgta gatttgtata ggccagatga ttactatgat
attttatttc ctggagtaca aacctttgtt cacagtgttc agtatcttga ccccagacat
tggggtccaa cactttttaa tgccatttct caagcttttt ggcgtgtaat acaaaatgac
attcctaggc tcacctcaca ggagcttgaa agaagaaccc aaagatattt aagggacagt
ttggcaaggt ttttagagga aactacttgg acagtaatta atgctcctgt taattggtat
aactctttac aagattacta ctctactttg tctcccatta ggcctacaat ggtgagacaa
gtagccaaca gggaagggtt gcaaatatca tttgggcaca cctatgataa tattgatgaa
gcagacagta ttcagcaagt aactgagagg tgggaagctc aaagccaaag tcctaatgtg
cagtcaggtg aatttattga aaaatttgag gctcctggtg gtgcaaatca aagaactgct
cctcagtgga tgttgccttt acttctaggc ctgtacggaa gtgttacttc tgctctaaaa
gcttatgaag atggccccaa caaaaagaaa aggaagttgt ccaggggcag ctcccaaaaa
accaaaggaa ccagtgcaag tgccaaagct cgtcataaaa ggaggaatag aagttctagg
agttaaaact ggagtagaca gcttcactga ggtggagtgc tttttaaatc ctcaaatggg
caatcctgat gaacatcaaa aaggcttaag taaaagctta gcagctgaaa aacagtttac
agatgactct ccagacaaag aacaactgcc ttgctacagt gtggctagaa ttcctttgcc
taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg ttactgttaa
aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac aaaaaactca
tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg ctgttggtgg
ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc ctgctcaaac
tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc acaaggctgt
tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa gtaaaaatga
aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg ttttgcacat
tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct tgtgcagatc
tcccgggtct agataagtaa taagctgaca gcttgtatgt ttctgctgtt gacatttgtg
ggctgtttac caacacttct ggaacacagc agtggaaggg acttcccaga tattttaaaa
ttacccttag aaagcggtct gtgaaaaacc cctacccaat ttcctttttg ttaagtgacc
taattaacag gaggacacag agggtggatg ggcagcctat gattggaatg tcctctcaag
tagaggaggt tagggtttat gaggacacag aggagcttcc tggggatcct ctacgccgga
cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
gagtcgcata agggagagcg tcgagatcta agctttgcaa agatggataa agcggaatta
attcccgagc ctccaaaaaa gaagagaaag gtcgaattgg gtaccgccgc caattttaat
caaagtggga atattgctga tagctcattg tccttcactt tcactaacag tagcaacggt
ccgaacctca taacaactca aacaaattct caagcgcttt cacaaccaat tgcctcctct
aacgttcatg ataacttcat gaataatgaa atcacggcta gtaaaattga tgatggtaat
aattcaaaac cactgtcacc tggttggacg gaccaaactg cgtataacgc gtttggaatc
actacaggga tgtttaatac cactacaatg gatgatgtat ataactatct attcgatgat
gaagataccc caccaaaccc aaaaaaagag atcgaattcg agcttatgcg gtgtgaaata
ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtct
//