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ID   PSG925     preliminary; circular DNA; SYN; 5508 BP.
XX
AC   U14127;
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DT   13-SEP-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli vector pSG925 - complete.
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KW   cloning vector; HIS4 gene.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5508
RC   pSG925, pSG926, pSG927, pSG928, pSG929 from Pichia pastoris HIS4 gene
RC   pSG930, pSG931, pSG932, pSG933, pSG934 from Pichia pastoris HIS4 gene
RC   pSG935 from Pichia pastoris HIS4 gene
RA   Gould S.J.;
RT   "The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a
RT   non-reverting his4 deletion mutant, and development of HIS4-based
RT   replicating and integrating plasmids";
RL   Curr. Genet. 26:0-0(1994).
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RN   [2]
RP   1-5508
RC   pSG925
RA   Gould S.J.;
RT   ;
RL   Submitted (29-AUG-1994) by:
RL   Gould S.J., Neurogenetics, The Kennedy Krieger Institute,
RL   707 North Broadway, Baltimore, MD 21030, USA.
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CC   NCBI gi: 537485
CC   NM (pSG925)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. Pichia pastoris, HIS4 gene
FT                   -> pSG925 5508bp"
FT   CDS             complement(1861..2721)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amr)"
FT   CDS             2821..5349
FT                   /note="GEN histidinol dehydrogenase gene"
XX
SQ   Sequence 5508 BP; 1470 A; 1258 C; 1281 G; 1499 T; 0 other;
     cgaccaatta catacccctc atagaactac gttggaacat agtccgtccc cgggggaaga
     tttattgtct caaaaggtca atttcatatt ttatatgcat tcaatactta tttattatta
     atttagcttg actacgatgc atataatttt aattttattt taaattatat atgaggtaag
     agtataactc taaacctaat aaatatataa ttaattatac gcaatagtta aaccatagat
     taattacaac taatcctttc gtactaagtt gtaatccttt attgacattt ccctaaagca
     gatagaaacc atactgtctc acgactatta aacccaactc acgtaacctt ttaattgacg
     aacagtcaaa cccttatcag cgtgtgctac caataggata ggttgagtcg acatcgacat
     atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcggtacccg gggatcctct
     agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     acggaaatgt tgaatactca tactcttcct ttttcaatga tctcctgatg actgactcac
     tgataataaa aatacggctt cagaatttct caagactaca ctcactgtcc gacttcaagt
     atgacatttc ccttgctacc tgcatacgca agtgttgcag agtttgataa ttccttgagt
     ttggtaggaa aagccgtgtt tccctatgct gctgaccagc tgcacaacct gatcaagttc
     actcaatcga ctgagcttca agttaatgtg caagttgagt catccgttac agaggaccaa
     tttgaggagc tgatcgacaa cttgctcaag ttgtacaata atggtatcaa tgaagtgatt
     ttggacctag atttggcaga aagagttgtc caaaggatcc caggcgctag ggttatctat
     aggaccctgg ttgataaagt tgcatccttg cccgctaatg ctagtatcgc tgtgcctttt
     tcttctccac tgggcgattt gaaaagtttc actaatggcg gtagtagaac tgtttatgct
     ttttctgaga ccgcaaagtt ggtagatgtg acttccactg ttgcttctgg tataatcccc
     attattgatg ctcggcaatt gactactgaa tacgaacttt ctgaagatgt caaaaagttc
     cctgtcagtg aaattttgtt ggcgtctttg actactgacc gccccgatgg tctattcact
     actttggtgg ctgactcttc taattactcg ttgggcctgg tgtactcgtc caaaaagtct
     attccggagg ctataaggac acaaactgga gtctaccaat ctcgtcgtca cggtttgtgg
     tataaaggtg ctacatctgg agcaactcaa aagttgctgg gtatcgaatt ggattgtgat
     ggagactgct tgaaatttgt ggttgaacaa acaggtgttg gtttctgtca cttggaacgc
     acttcctgtt ttggccaatc aaagggtctt agagccatgg aagccacctt gtgggatcgt
     aagagcaatg ctccagaagg ttcttatacc aaacggttat ttgacgacga agttttgttg
     aacgctaaaa ttagggagga agctgatgaa cttgcagaag ctaaatccaa ggaagatata
     gcctgggaat gtgctgactt attttatttt gcattagtta gatgtgccaa gtacggcgtg
     acgttggacg aggtggagag aaacctggat atgaagtccc taaaggtcac tagaaggaaa
     ggagatgcca agccaggata caccaaggaa caacctaaag aagaatccaa acctaaagaa
     gtcccttctg aaggtcgtat tgaattgtgc aaaattgacg tttctaaggc ctcctcacaa
     gaaattgaag atgcccttcg tcgtcctatc cagaaaacgg aacagattat ggaattagtc
     aaaccaattg tcgacaatgt tcgtcaaaat ggtgacaaag cccttttaga actaactgcc
     aagtttgatg gagtcgcttt gaagacacct gtgttagaag ctcctttccc agaggaactt
     atgcaattgc cagataacgt taagagagcc attgatctct ctatagataa cgtcaggaaa
     ttccatgaag ctcaactagc ggagacgttg caagttgaga cttgccctgg tgtagtctgc
     tctcgttttg caagacctat tgagaaagtt ggcctctata ttcctggtgg aaccgcaatt
     ctgccttcca cttccctgat gctgggtgtt cctgccaaag ttgctggttg caaagaaatt
     gtttttgcat ctccacctaa gaaggatggt acccttaccc cagaagtcat ctacgttgcc
     cacaaggttg gtgctaagtg tatcgtgcta gcaggaggcg cccaggcagt agctgctatg
     gcttacggaa cagaaactgt tcctaagtgt gacaaaatat ttggtccagg aaaccagttc
     gttactgctg ccaagatgat ggttcaaaat gacacatcag ccctgtgtag tattgacatg
     cctgctgggc cttctgaagt tctagttatt gctgataaat acgctgatcc agatttcgtt
     gtctcagacc ttctgtctca agctgaacat ggtattgatt cccaggtgat tctgttggct
     gtcgatatga cagacaagga gcttgccaga attgaagatg ctgttcacaa ccaagctgtg
     cagttgccaa gggttgaaat tgtacgcaag tgtattgcac actctacaac cctatcggtt
     gcaacctacg agcaggcttt ggaaatgtcc aatcagtacg ctcctgaaca cttgatcctg
     caaatcgaga atgcttctta tgttgatcaa gtacaacacg ctggatctgt gtttgttggt
     gcctactctc cagagagttg tggagattac tcctccggta ccaaccacac tttgccaacg
     tacggatatg cccgtcaata cagcggagtt aacactgcaa ccttccagaa gttcatcact
     tcacaagacg taactcctga gggactgaaa catattggcc aagcagtgat ggatctggct
     gctgttgaag gtctagatgc tcaccgcaat gctgttaagg ttcgtatgga gaaactggga
     cttatttaat tatttagaga ttttaactta catttagatt cgatagatca ttattgaagc
     atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//